TOP TEN perturbations for 1553567_s_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553567_s_at
Selected probe(set): 1553570_x_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553567_s_at (1553570_x_at) across 6672 perturbations tested by GENEVESTIGATOR:

sapphyrin PCI-2050 study 1 / mannitol treated A549 cell sample

Relative Expression (log2-ratio):-3.8418417
Number of Samples:3 / 3
Experimental sapphyrin PCI-2050 study 1
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. ATC code:---
Control mannitol treated A549 cell sample
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture.

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-3.7224474
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

sapphyrin PCI-2050 study 2 / mannitol treated A549 cell sample

Relative Expression (log2-ratio):-3.4111385
Number of Samples:2 / 3
Experimental sapphyrin PCI-2050 study 2
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. ATC code:---
Control mannitol treated A549 cell sample
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture.

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-2.2635841
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):2.0559177
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):2.0253983
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81) / hypoxia study 11 (AB81)

Relative Expression (log2-ratio):1.9709206
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.
Control hypoxia study 11 (AB81)
Human podocyte cell line AB81 treated with hypoxia (1% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.

glioma study 16 (LN-319) / normal astrocyte sample

Relative Expression (log2-ratio):1.8746796
Number of Samples:2 / 3
Experimental glioma study 16 (LN-319)
Human glioma cell line LN319 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

hypoxia study 11 (AB81) / control AB81 cell sample (normoxia)

Relative Expression (log2-ratio):-1.8532887
Number of Samples:3 / 3
Experimental hypoxia study 11 (AB81)
Human podocyte cell line AB81 treated with hypoxia (1% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.
Control control AB81 cell sample (normoxia)
Human podocyte cell line AB81 treated with normoxia (20% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):1.847393
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

Organism: Homo sapiens
Gene: 1553567_s_at
Selected probe(set): 1553588_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553567_s_at (1553588_at) across 6672 perturbations tested by GENEVESTIGATOR:

HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81) / hypoxia study 11 (AB81)

Relative Expression (log2-ratio):2.1322489
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.
Control hypoxia study 11 (AB81)
Human podocyte cell line AB81 treated with hypoxia (1% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.

idiopathic pulmonary fibrosis study 9 (non-contractile; ribo. bound RNA) / idiopathic pulmonary fibrosis study 9 (non-contractile; total RNA)

Relative Expression (log2-ratio):-2.0026894
Number of Samples:6 / 6
Experimental idiopathic pulmonary fibrosis study 9 (non-contractile; ribo. bound RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control idiopathic pulmonary fibrosis study 9 (non-contractile; total RNA)
Primary lung myofibroblasts explanted from patients with idiopathic pulmonary fibrosis (IPF) grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from IPF patients (age range 57-68 years) at the time of biopsy, autopsy, lung resection or lung transplantation. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

non-contractile lung myofibroblast study 1 (ribo. bound RNA) / non-contractile lung myofibroblast study 1 (total RNA)

Relative Expression (log2-ratio):-1.9510403
Number of Samples:6 / 6
Experimental non-contractile lung myofibroblast study 1 (ribo. bound RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control non-contractile lung myofibroblast study 1 (total RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

hypoxia study 11 (AB81) / control AB81 cell sample (normoxia)

Relative Expression (log2-ratio):-1.915616
Number of Samples:3 / 3
Experimental hypoxia study 11 (AB81)
Human podocyte cell line AB81 treated with hypoxia (1% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.
Control control AB81 cell sample (normoxia)
Human podocyte cell line AB81 treated with normoxia (20% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.

polycystic ovary syndrome study 6 / normal granulosa cell sample

Relative Expression (log2-ratio):1.7380733
Number of Samples:5 / 2
Experimental polycystic ovary syndrome study 6
Granulosa cell sample derived from women with polycystic ovary syndrome (PCOS) after controlled ovarian hyperstimulation, as a part of standard in vitro fertilization (IVF) protocol. The PCOS was diagnosed according to Rotterdam revised criteria. All subjects were < 35 years with normal prolactin levels and normal thyroid function.
Control normal granulosa cell sample
Granulosa cell sample derived from normal ovulatory women after controlled ovarian hyperstimulation, as a part of standard in vitro fertilization (IVF) protocol. The reason for IVF was male or tubal factor infertility. All subjects were < 35 years with normal prolactin levels, normal thyroid function, regular menstrual cycle, no clinical or biochemical evidence of hyperandrogenism, no polycystic ovaries and normal insulin sensitivity.

HIF-2a depletion study 1 (hypoxia; AB81) / hypoxia study 11 (AB81)

Relative Expression (log2-ratio):1.7332029
Number of Samples:3 / 3
Experimental HIF-2a depletion study 1 (hypoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-2a (Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.
Control hypoxia study 11 (AB81)
Human podocyte cell line AB81 treated with hypoxia (1% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.

CAR T cell study 4 (PSCA-28t28Z; post-infusion) / CAR T cell study 4 (PSCA-28t28Z; pre-infusion)

Relative Expression (log2-ratio):1.674427
Number of Samples:3 / 3
Experimental CAR T cell study 4 (PSCA-28t28Z; post-infusion)
CD8+ T cells transduced with PSCA-28t28Z (second generation CAR) and isolated 30 days after adoptive transfer into mice bearing HPAC-derived pancreatic tumor. Human peripheral blood mononuclear cells were stimulated with anti-CD3 antibody (OKT3) in presence of IL-2. Two days post-stimulation, T cells were transduced with retroviral vectors encoding PSCA-28t28Z. Cells were cultured for 2 weeks in presence of IL-2 and then transfered into 4-5-week-old male NSG mice. Subcutaneous xenografts were generated by injection of HPAC cells. Once tumors became palpable, mice were treated with CD8+ T cells expressing PSCA-28t28Z. Untransduced CD4+ cells from the same donor were given to each mouse for cytokine support. Spleen-resident human CD8+ T cells were isolated 30 days later using the CD8 MicroBeads (post-infusion samples).
Control CAR T cell study 4 (PSCA-28t28Z; pre-infusion)
Primary human CD8+ T cells stimulated ex vivo and transduced to express PSCA-28t28Z (second generation CAR). Human peripheral blood mononuclear cells were stimulated with anti-CD3 antibody (OKT3) in presence of IL-2. Two days post-stimulation, T cells were transduced with retroviral vectors encoding PSCA-28t28Z. Cells were cultured for 2 weeks in presence of IL-2, until collection of samples (pre-infusion samples).

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-1.6359844
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).

plicamycin study 2 (100 nM) / vehicle (PBS) treated TC-71 cell sample

Relative Expression (log2-ratio):-1.6105022
Number of Samples:3 / 3
Experimental plicamycin study 2 (100 nM)
TC-71 Ewing´s sarcoma cells treated with compound: plicamycin (mithramycin, 100 nM) for 6 hours. ATC code:
Control vehicle (PBS) treated TC-71 cell sample
TC-71 Ewing´s sarcoma cells treated with 0.01% phosphate-buffered saline (PBS) in growth medium for 6 hours.

HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81) / HIF-1a depletion study 2 (hypoxia; AB81)

Relative Expression (log2-ratio):1.5773325
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.
Control HIF-1a depletion study 2 (hypoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.

Organism: Homo sapiens
Gene: 1553567_s_at
Selected probe(set): 1553567_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553567_s_at (1553567_s_at) across 6672 perturbations tested by GENEVESTIGATOR:

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-4.0538683
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

interferon-alpha-kinoid study 1 (240ug; 168d) / untreated whole blood sample

Relative Expression (log2-ratio):-1.9079514
Number of Samples:2 / 43
Experimental interferon-alpha-kinoid study 1 (240ug; 168d)
Whole blood samples of patients with systemic lupus erythematosus after treatment with interferon-alpha-kinoid (INF-K; inactivated IFNa coupled keyhole limpet hemocyanin as carrier). Patients had 3 doses of 240ug INK-K at day 0, 7 and 58. Sample was taken 168 days after the first application of TNF-K. ATC code:---
Control untreated whole blood sample
Whole blood samples from healthy volunteers.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):1.7333393
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

expO thyroid gland cancer study 1 (papillary carcinoma, NOS; primary) / expO thyroid gland cancer study 1 (follicular adenocarcinoma, NOS; primary)

Relative Expression (log2-ratio):1.7251816
Number of Samples:25 / 2
Experimental expO thyroid gland cancer study 1 (papillary carcinoma, NOS; primary)
Primary tumor tissue samples obtained from the thyroid gland of patients with papillary carcinoma (NOS).
Control expO thyroid gland cancer study 1 (follicular adenocarcinoma, NOS; primary)
Primary tumor tissue sample obtained from the thyroid gland of a patient with follicular adenocarcinoma (NOS).

non-contractile lung myofibroblast study 1 (ribo. bound RNA) / non-contractile lung myofibroblast study 1 (total RNA)

Relative Expression (log2-ratio):-1.6747437
Number of Samples:6 / 6
Experimental non-contractile lung myofibroblast study 1 (ribo. bound RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control non-contractile lung myofibroblast study 1 (total RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

colorectal cancer study 33 (carcinoma; colonic mucosa) / normal colon mucosa sample

Relative Expression (log2-ratio):1.6664219
Number of Samples:5 / 5
Experimental colorectal cancer study 33 (carcinoma; colonic mucosa)
Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal carcinoma.
Control normal colon mucosa sample
Histopathological normal colon mucosa sample obtained by laser capture microdissection (LCM) from the distant normal colon of patients with colorectal cancer.

atopic dermatitis study 21 (lesional; whole skin) / normal skin tissue

Relative Expression (log2-ratio):-1.6015701
Number of Samples:5 / 6
Experimental atopic dermatitis study 21 (lesional; whole skin)
Lesional full thickness skin samples isolated from patient with moderate-to-severe atopic dermatitis by laser capture microdissection. Patients' cohort characteristics: 3 males and 2 females; age 27-59 years (mean age: 39.4 years); SCORing of Atopic Dermatitis index (SCORAD) ranging from 45-65; total IgE: 14-1821 kU/l; eosinophilic count: 1.4-11.8 %.
Control normal skin tissue
Full thickness skin samples isolated from healthy subjects by laser capture microdissection.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):1.5697536
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

colorectal adenoma study 7 (colonic mucosa) / normal colon mucosa sample

Relative Expression (log2-ratio):1.5697422
Number of Samples:5 / 5
Experimental colorectal adenoma study 7 (colonic mucosa)
Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal adenoma.
Control normal colon mucosa sample
Histopathological normal colon mucosa sample obtained by laser capture microdissection (LCM) from the distant normal colon of patients with colorectal cancer.

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-1.55723
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).

Organism: Homo sapiens
Gene: 1553567_s_at
Selected probe(set): 1553567_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553567_s_at (1553567_s_at) across 6672 perturbations tested by GENEVESTIGATOR:

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-4.0538683
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

interferon-alpha-kinoid study 1 (240ug; 168d) / untreated whole blood sample

Relative Expression (log2-ratio):-1.9079514
Number of Samples:2 / 43
Experimental interferon-alpha-kinoid study 1 (240ug; 168d)
Whole blood samples of patients with systemic lupus erythematosus after treatment with interferon-alpha-kinoid (INF-K; inactivated IFNa coupled keyhole limpet hemocyanin as carrier). Patients had 3 doses of 240ug INK-K at day 0, 7 and 58. Sample was taken 168 days after the first application of TNF-K. ATC code:---
Control untreated whole blood sample
Whole blood samples from healthy volunteers.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):1.7333393
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

expO thyroid gland cancer study 1 (papillary carcinoma, NOS; primary) / expO thyroid gland cancer study 1 (follicular adenocarcinoma, NOS; primary)

Relative Expression (log2-ratio):1.7251816
Number of Samples:25 / 2
Experimental expO thyroid gland cancer study 1 (papillary carcinoma, NOS; primary)
Primary tumor tissue samples obtained from the thyroid gland of patients with papillary carcinoma (NOS).
Control expO thyroid gland cancer study 1 (follicular adenocarcinoma, NOS; primary)
Primary tumor tissue sample obtained from the thyroid gland of a patient with follicular adenocarcinoma (NOS).

non-contractile lung myofibroblast study 1 (ribo. bound RNA) / non-contractile lung myofibroblast study 1 (total RNA)

Relative Expression (log2-ratio):-1.6747437
Number of Samples:6 / 6
Experimental non-contractile lung myofibroblast study 1 (ribo. bound RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control non-contractile lung myofibroblast study 1 (total RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

colorectal cancer study 33 (carcinoma; colonic mucosa) / normal colon mucosa sample

Relative Expression (log2-ratio):1.6664219
Number of Samples:5 / 5
Experimental colorectal cancer study 33 (carcinoma; colonic mucosa)
Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal carcinoma.
Control normal colon mucosa sample
Histopathological normal colon mucosa sample obtained by laser capture microdissection (LCM) from the distant normal colon of patients with colorectal cancer.

atopic dermatitis study 21 (lesional; whole skin) / normal skin tissue

Relative Expression (log2-ratio):-1.6015701
Number of Samples:5 / 6
Experimental atopic dermatitis study 21 (lesional; whole skin)
Lesional full thickness skin samples isolated from patient with moderate-to-severe atopic dermatitis by laser capture microdissection. Patients' cohort characteristics: 3 males and 2 females; age 27-59 years (mean age: 39.4 years); SCORing of Atopic Dermatitis index (SCORAD) ranging from 45-65; total IgE: 14-1821 kU/l; eosinophilic count: 1.4-11.8 %.
Control normal skin tissue
Full thickness skin samples isolated from healthy subjects by laser capture microdissection.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):1.5697536
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

colorectal adenoma study 7 (colonic mucosa) / normal colon mucosa sample

Relative Expression (log2-ratio):1.5697422
Number of Samples:5 / 5
Experimental colorectal adenoma study 7 (colonic mucosa)
Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal adenoma.
Control normal colon mucosa sample
Histopathological normal colon mucosa sample obtained by laser capture microdissection (LCM) from the distant normal colon of patients with colorectal cancer.

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-1.55723
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).

Organism: Homo sapiens
Gene: 1553567_s_at
Selected probe(set): 1553567_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553567_s_at (1553567_s_at) across 6672 perturbations tested by GENEVESTIGATOR:

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-4.0538683
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

interferon-alpha-kinoid study 1 (240ug; 168d) / untreated whole blood sample

Relative Expression (log2-ratio):-1.9079514
Number of Samples:2 / 43
Experimental interferon-alpha-kinoid study 1 (240ug; 168d)
Whole blood samples of patients with systemic lupus erythematosus after treatment with interferon-alpha-kinoid (INF-K; inactivated IFNa coupled keyhole limpet hemocyanin as carrier). Patients had 3 doses of 240ug INK-K at day 0, 7 and 58. Sample was taken 168 days after the first application of TNF-K. ATC code:---
Control untreated whole blood sample
Whole blood samples from healthy volunteers.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):1.7333393
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

expO thyroid gland cancer study 1 (papillary carcinoma, NOS; primary) / expO thyroid gland cancer study 1 (follicular adenocarcinoma, NOS; primary)

Relative Expression (log2-ratio):1.7251816
Number of Samples:25 / 2
Experimental expO thyroid gland cancer study 1 (papillary carcinoma, NOS; primary)
Primary tumor tissue samples obtained from the thyroid gland of patients with papillary carcinoma (NOS).
Control expO thyroid gland cancer study 1 (follicular adenocarcinoma, NOS; primary)
Primary tumor tissue sample obtained from the thyroid gland of a patient with follicular adenocarcinoma (NOS).

non-contractile lung myofibroblast study 1 (ribo. bound RNA) / non-contractile lung myofibroblast study 1 (total RNA)

Relative Expression (log2-ratio):-1.6747437
Number of Samples:6 / 6
Experimental non-contractile lung myofibroblast study 1 (ribo. bound RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control non-contractile lung myofibroblast study 1 (total RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

colorectal cancer study 33 (carcinoma; colonic mucosa) / normal colon mucosa sample

Relative Expression (log2-ratio):1.6664219
Number of Samples:5 / 5
Experimental colorectal cancer study 33 (carcinoma; colonic mucosa)
Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal carcinoma.
Control normal colon mucosa sample
Histopathological normal colon mucosa sample obtained by laser capture microdissection (LCM) from the distant normal colon of patients with colorectal cancer.

atopic dermatitis study 21 (lesional; whole skin) / normal skin tissue

Relative Expression (log2-ratio):-1.6015701
Number of Samples:5 / 6
Experimental atopic dermatitis study 21 (lesional; whole skin)
Lesional full thickness skin samples isolated from patient with moderate-to-severe atopic dermatitis by laser capture microdissection. Patients' cohort characteristics: 3 males and 2 females; age 27-59 years (mean age: 39.4 years); SCORing of Atopic Dermatitis index (SCORAD) ranging from 45-65; total IgE: 14-1821 kU/l; eosinophilic count: 1.4-11.8 %.
Control normal skin tissue
Full thickness skin samples isolated from healthy subjects by laser capture microdissection.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):1.5697536
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

colorectal adenoma study 7 (colonic mucosa) / normal colon mucosa sample

Relative Expression (log2-ratio):1.5697422
Number of Samples:5 / 5
Experimental colorectal adenoma study 7 (colonic mucosa)
Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal adenoma.
Control normal colon mucosa sample
Histopathological normal colon mucosa sample obtained by laser capture microdissection (LCM) from the distant normal colon of patients with colorectal cancer.

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-1.55723
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).

Organism: Homo sapiens
Gene: 1553567_s_at
Selected probe(set): 1553567_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553567_s_at (1553567_s_at) across 6672 perturbations tested by GENEVESTIGATOR:

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-4.0538683
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

interferon-alpha-kinoid study 1 (240ug; 168d) / untreated whole blood sample

Relative Expression (log2-ratio):-1.9079514
Number of Samples:2 / 43
Experimental interferon-alpha-kinoid study 1 (240ug; 168d)
Whole blood samples of patients with systemic lupus erythematosus after treatment with interferon-alpha-kinoid (INF-K; inactivated IFNa coupled keyhole limpet hemocyanin as carrier). Patients had 3 doses of 240ug INK-K at day 0, 7 and 58. Sample was taken 168 days after the first application of TNF-K. ATC code:---
Control untreated whole blood sample
Whole blood samples from healthy volunteers.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):1.7333393
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

expO thyroid gland cancer study 1 (papillary carcinoma, NOS; primary) / expO thyroid gland cancer study 1 (follicular adenocarcinoma, NOS; primary)

Relative Expression (log2-ratio):1.7251816
Number of Samples:25 / 2
Experimental expO thyroid gland cancer study 1 (papillary carcinoma, NOS; primary)
Primary tumor tissue samples obtained from the thyroid gland of patients with papillary carcinoma (NOS).
Control expO thyroid gland cancer study 1 (follicular adenocarcinoma, NOS; primary)
Primary tumor tissue sample obtained from the thyroid gland of a patient with follicular adenocarcinoma (NOS).

non-contractile lung myofibroblast study 1 (ribo. bound RNA) / non-contractile lung myofibroblast study 1 (total RNA)

Relative Expression (log2-ratio):-1.6747437
Number of Samples:6 / 6
Experimental non-contractile lung myofibroblast study 1 (ribo. bound RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed only with ribosome-bound RNA.
Control non-contractile lung myofibroblast study 1 (total RNA)
Primary lung myofibroblasts explanted from control individuals grown in a 3-dimensional type I collagen matrix and not allowed to contract. Tissues were obtained from cancer patients (age range 56-82 years) at the time of tumor resection. The analyzed lung resection was histologically normal and distant from tumor tissue. Primary cells outgrowth were cultured in DMEM growth medium (10% FCS) and passaged at a 1:4 split ratio until passage 4 to 9. Cells were considered myofibroblasts if they had a typical spindle morphology, if they were vimentin- and alpha smooth muscle actin-positive and factor VIII and surfactant C-negative. To simulate an aberrant, fibrotic environment, the 3-dimensional type I collagen gel was fixed at the border of the dish to not allow primary cells to freely contract (non-contractile matrices). Microarray was performed with total RNA.

colorectal cancer study 33 (carcinoma; colonic mucosa) / normal colon mucosa sample

Relative Expression (log2-ratio):1.6664219
Number of Samples:5 / 5
Experimental colorectal cancer study 33 (carcinoma; colonic mucosa)
Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal carcinoma.
Control normal colon mucosa sample
Histopathological normal colon mucosa sample obtained by laser capture microdissection (LCM) from the distant normal colon of patients with colorectal cancer.

atopic dermatitis study 21 (lesional; whole skin) / normal skin tissue

Relative Expression (log2-ratio):-1.6015701
Number of Samples:5 / 6
Experimental atopic dermatitis study 21 (lesional; whole skin)
Lesional full thickness skin samples isolated from patient with moderate-to-severe atopic dermatitis by laser capture microdissection. Patients' cohort characteristics: 3 males and 2 females; age 27-59 years (mean age: 39.4 years); SCORing of Atopic Dermatitis index (SCORAD) ranging from 45-65; total IgE: 14-1821 kU/l; eosinophilic count: 1.4-11.8 %.
Control normal skin tissue
Full thickness skin samples isolated from healthy subjects by laser capture microdissection.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):1.5697536
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

colorectal adenoma study 7 (colonic mucosa) / normal colon mucosa sample

Relative Expression (log2-ratio):1.5697422
Number of Samples:5 / 5
Experimental colorectal adenoma study 7 (colonic mucosa)
Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal adenoma.
Control normal colon mucosa sample
Histopathological normal colon mucosa sample obtained by laser capture microdissection (LCM) from the distant normal colon of patients with colorectal cancer.

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-1.55723
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).