TOP TEN perturbations for 1553630_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553630_at
Selected probe(set): 1553630_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553630_at (1553630_at) across 6672 perturbations tested by GENEVESTIGATOR:

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):4.4027987
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)

Relative Expression (log2-ratio):4.395636
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (confluent)
Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert.

ovarian tumor study 11 (high grade) / ovarian tumor study 11 (borderline)

Relative Expression (log2-ratio):-2.1371808
Number of Samples:22 / 8
Experimental ovarian tumor study 11 (high grade)
Human microdissected tumor cells from the ovary of patients with high grade serous carcinoma.
Control ovarian tumor study 11 (borderline)
Human microdissected tumor cells from the ovary of patients with low-malignant (borderline) tumors of the ovary.

chronic obstructive pulmonary disease study 30 / normal nasal epithelium tissue (never-smoker)

Relative Expression (log2-ratio):1.8571377
Number of Samples:50 / 54
Experimental chronic obstructive pulmonary disease study 30
Nasal epithelium obtained from patients who suffered from COPD GOLD I or II (mild-to-moderate COPD) and were current smokers. Subjects had early-stage COPD (FEV during the first second [FEV1]/ forced vital capacity [FVC] 70 %) and had a smoking history of at least 10 pack/years. Subjects in each of the groups were matched to subjects in the COPD group by age (±5 years), ethnicity, and sex using a match ID.
Control normal nasal epithelium tissue (never-smoker)
Nasal epithelium obtained from never smokers (NS). Subjects in each of the groups were matched to subjects in the COPD group by age (±5 years), ethnicity, and sex using a match ID.

ovarian tumor study 11 (low grade) / ovarian tumor study 11 (borderline)

Relative Expression (log2-ratio):-1.8394241
Number of Samples:11 / 8
Experimental ovarian tumor study 11 (low grade)
Human microdissected tumor cells from the ovary of patients with low grade serous carcinoma.
Control ovarian tumor study 11 (borderline)
Human microdissected tumor cells from the ovary of patients with low-malignant (borderline) tumors of the ovary.

ovarian tumor study 11 (borderline) / normal ovarian surface epithelial cell sample

Relative Expression (log2-ratio):1.7847757
Number of Samples:8 / 6
Experimental ovarian tumor study 11 (borderline)
Human microdissected tumor cells from the ovary of patients with low-malignant (borderline) tumors of the ovary.
Control normal ovarian surface epithelial cell sample
Human microdissected ovarian surface epithelial cell sample from the ovary of healthy individuals.

Jurkat-PSIP1 KD / Jurkat

Relative Expression (log2-ratio):-1.7828007
Number of Samples:3 / 3
Experimental Jurkat-PSIP1 KD
Jurkat cells stably transfected with shRNA constructs (si1340) against LEDGF transcript (PSIP1 gene). Jurkat cells are human relapse cancer cell line derived from the peripheral blood of a patient with acute lymphoblastic leukemia. Parental cell line:: Jurkat Synonyms:Jurkat-siJK2
Control Jurkat
Human relapse cancer cell line derived from the peripheral blood of a patient with acute lymphoblastic leukemia. Synonyms:JURKAT; JM; JM-Jurkat; Jurkat-FHCRC; Jurkat FHCRC; FHCRC-11; FHCRC subclone 11; FCCH1024 Cellosaurus code:

mechanical injury study 1 (smoker; 7d) / mechanical injury study 1 (smoker; 0d)

Relative Expression (log2-ratio):-1.719286
Number of Samples:4 / 6
Experimental mechanical injury study 1 (smoker; 7d)
Bronchial epithelial cells of the large (2nd - 3rd order) bronchi from healthy smokers were sampled 7 days after mechanical injury by airway brushing during bronchoscopy. Disposable brushes (2.0 mm) were used to injure airway epithelial cells by gently gliding the brush back and forth 10 times in different locations in the same general area. Individuals were determined to be phenotypically normal based on standard history, physical exam, complete blood count, coagulation studies, liver function tests, urine studies, chest X-ray, EKG, and pulmonary function tests.
Control mechanical injury study 1 (smoker; 0d)
Bronchial epithelial cells of the large (2nd - 3rd order) bronchi from healthy smokers were sampled at rest before airway brushing (day 0). Disposable brushes (2.0 mm) were used to injure airway epithelial cells by gently gliding the brush back and forth 10 times in different locations in the same general area. Individuals were determined to be phenotypically normal based on standard history, physical exam, complete blood count, coagulation studies, liver function tests, urine studies, chest X-ray, EKG, and pulmonary function tests.

brain tumor study 1 (medulloblastoma) / brain tumor study 1 (ependymoma)

Relative Expression (log2-ratio):-1.7075677
Number of Samples:22 / 46
Experimental brain tumor study 1 (medulloblastoma)
Primary tumor tissue sample from the brain of patients with medulloblastoma.
Control brain tumor study 1 (ependymoma)
Primary tumor tissue sample from the brain of patients with ependymoma.

rheumatoid arthritis study 69 (TNF; 20h; 1ng/ml) / rheumatoid arthritis study 69 (TNF; IL-17A; baseline)

Relative Expression (log2-ratio):-1.6222763
Number of Samples:6 / 6
Experimental rheumatoid arthritis study 69 (TNF; 20h; 1ng/ml)
Joint synovial fibroblast samples from patients with rheumatoid arthritis after treatment with 1ng/ml TNF for 20 hours. Synovial fibroblasts were isolated from tissues discarded after synovectomy or joint replacement and isolated from digested synovial tissue and sorted for enriched CD45−, CD31−, CD235a−, Pdpn+ surface proteins. Cells were maintained in DMEM supplemented with 10% FBS, 2 mM L-glutamine, 50 uM 2-mercaptoethanol, antibiotics (penicillin and streptomycin), and essential and nonessential amino acids and used between passages 5 and 8 for treatment.
Control rheumatoid arthritis study 69 (TNF; IL-17A; baseline)
Joint synovial fibroblast samples at baseline from patients with rheumatoid arthritis before treatment with 1ng/ml TNF and/or IL-17 for 20 hours. Synovial fibroblasts were isolated from tissues discarded after synovectomy or joint replacement and prepared from digested synovial tissue and sorted for enriched CD45−, CD31−, CD235a−, Pdpn+ surface proteins. Cells were maintained in DMEM supplemented with 10% FBS, 2 mM L-glutamine, 50 uM 2-mercaptoethanol, antibiotics (penicillin and streptomycin), and essential and nonessential amino acids and used between passages 5 and 8 for treatment.