TOP TEN perturbations for 1553655_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553655_at
Selected probe(set): 1553655_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553655_at (1553655_at) across 6672 perturbations tested by GENEVESTIGATOR:

succinate dehydrogenase B depletion study 1 (siRNA) / control siRNA transfected Hep3B cell sample

Relative Expression (log2-ratio):6.801811
Number of Samples:3 / 3
Experimental succinate dehydrogenase B depletion study 1 (siRNA)
Hep3B cells stably transfected with short hairpin vectors containing an siRNA directed to the subunit B of the succinate dehydrogenase.
Control control siRNA transfected Hep3B cell sample
Hep3B cells stably transfected with empty siRNA expression vector (pU6).

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)

Relative Expression (log2-ratio):5.020947
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (confluent)
Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):5.0167003
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

mechanical injury study 1 (smoker; 7d) / mechanical injury study 1 (smoker; 0d)

Relative Expression (log2-ratio):4.0597134
Number of Samples:4 / 6
Experimental mechanical injury study 1 (smoker; 7d)
Bronchial epithelial cells of the large (2nd - 3rd order) bronchi from healthy smokers were sampled 7 days after mechanical injury by airway brushing during bronchoscopy. Disposable brushes (2.0 mm) were used to injure airway epithelial cells by gently gliding the brush back and forth 10 times in different locations in the same general area. Individuals were determined to be phenotypically normal based on standard history, physical exam, complete blood count, coagulation studies, liver function tests, urine studies, chest X-ray, EKG, and pulmonary function tests.
Control mechanical injury study 1 (smoker; 0d)
Bronchial epithelial cells of the large (2nd - 3rd order) bronchi from healthy smokers were sampled at rest before airway brushing (day 0). Disposable brushes (2.0 mm) were used to injure airway epithelial cells by gently gliding the brush back and forth 10 times in different locations in the same general area. Individuals were determined to be phenotypically normal based on standard history, physical exam, complete blood count, coagulation studies, liver function tests, urine studies, chest X-ray, EKG, and pulmonary function tests.

SDF study 4 / untreated MDA Mb231 cell sample

Relative Expression (log2-ratio):3.9745474
Number of Samples:3 / 6
Experimental SDF study 4
MDA Mb231 cells treated with 75nM stromal derived factor (SDF; Cxcl12) for 6h.
Control untreated MDA Mb231 cell sample
Untreated MDA Mb231 cells harvested after 6h.

mechanical injury study 1 (non-smoker; 7d) / mechanical injury study 1 (non-smoker; 0d)

Relative Expression (log2-ratio):3.814969
Number of Samples:3 / 4
Experimental mechanical injury study 1 (non-smoker; 7d)
Bronchial epithelial cells of the large (2nd - 3rd order) bronchi from healthy non-smokers were sampled 7 days after mechanical injury by airway brushing during bronchoscopy. Disposable brushes (2.0 mm) were used to injure airway epithelial cells by gently gliding the brush back and forth 10 times in different locations in the same general area. Individuals were determined to be phenotypically normal based on standard history, physical exam, complete blood count, coagulation studies, liver function tests, urine studies, chest X-ray, EKG, and pulmonary function tests.
Control mechanical injury study 1 (non-smoker; 0d)
Bronchial epithelial cells of the large (2nd - 3rd order) bronchi from healthy non-smokers were sampled at rest before airway brushing (day 0) during bronchoscopy. Disposable brushes (2.0 mm) were used to injure airway epithelial cells by gently gliding the brush back and forth 10 times in different locations in the same general area. Individuals were determined to be phenotypically normal based on standard history, physical exam, complete blood count, coagulation studies, liver function tests, urine studies, chest X-ray, EKG, and pulmonary function tests.

ovarian tumor study 11 (borderline) / normal ovarian surface epithelial cell sample

Relative Expression (log2-ratio):3.4411526
Number of Samples:8 / 6
Experimental ovarian tumor study 11 (borderline)
Human microdissected tumor cells from the ovary of patients with low-malignant (borderline) tumors of the ovary.
Control normal ovarian surface epithelial cell sample
Human microdissected ovarian surface epithelial cell sample from the ovary of healthy individuals.

ovarian tumor study 27 (papillary serous cystadenocarcinoma; metastase) / ovarian tumor study 27 (papillary serous cystadenocarcinoma; bmp; primary)

Relative Expression (log2-ratio):-2.898141
Number of Samples:53 / 18
Experimental ovarian tumor study 27 (papillary serous cystadenocarcinoma; metastase)
Metastatic tumor tissue samples obtained from different anatomical sites (colon, omentum, peritoneum and unstated sites) of female patients with primary papillary serous cystadenocarcinoma of the ovary.
Control ovarian tumor study 27 (papillary serous cystadenocarcinoma; bmp; primary)
Primary tumor tissue samples obtained from the ovary of female patients with papillary serous cystadenocarcinoma with borderline malignant potential (bmp).

ovarian tumor study 11 (high grade) / ovarian tumor study 11 (borderline)

Relative Expression (log2-ratio):-2.7815742
Number of Samples:22 / 8
Experimental ovarian tumor study 11 (high grade)
Human microdissected tumor cells from the ovary of patients with high grade serous carcinoma.
Control ovarian tumor study 11 (borderline)
Human microdissected tumor cells from the ovary of patients with low-malignant (borderline) tumors of the ovary.

ovarian tumor study 26 (serous carcinoma; metastase; peritoneum) / ovarian tumor study 26 (serous carcinoma; borderline; primary)

Relative Expression (log2-ratio):-2.7589312
Number of Samples:15 / 30
Experimental ovarian tumor study 26 (serous carcinoma; metastase; peritoneum)
Metastatic tumor tissue sample obtained from the peritoneum of female patients with primary serous cystadenocarcinoma of the ovary.
Control ovarian tumor study 26 (serous carcinoma; borderline; primary)
Ovarian tissue samples from female patients with primary serous cystadenoma with low malignant potential (borderline).