TOP TEN perturbations for 1553756_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553756_at
Selected probe(set): 1553756_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553756_at (1553756_at) across 6622 perturbations tested by GENEVESTIGATOR:

glioma study 17 (astrocytoma; A2B5+) / non-tumor oligodendrocyte progenitor cell sample (cortex)

Relative Expression (log2-ratio):-1.1750135
Number of Samples:3 / 3
Experimental glioma study 17 (astrocytoma; A2B5+)
Oligodendrocyte progenitor cells (OPC) isolated from low grade astrocytoma (grade II). OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen. Patients were 36 ± 7 years old.
Control non-tumor oligodendrocyte progenitor cell sample (cortex)
Oligodendrocyte progenitor cells (OPC) isolated from cortical tissue, which was obtained from patients with epilepsy, but without any manifested brain cancer. OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen.

brefeldin A study 1 (0.5ug/ml; p53HCT116) / untreated p53HCT116 cell sample

Relative Expression (log2-ratio):-1.1446342
Number of Samples:2 / 3
Experimental brefeldin A study 1 (0.5ug/ml; p53HCT116)
Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated p53HCT116 cell sample
Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

glioma study 17 (small cell glioblastoma; A2B5+) / non-tumor oligodendrocyte progenitor cell sample (cortex)

Relative Expression (log2-ratio):-1.1225529
Number of Samples:2 / 3
Experimental glioma study 17 (small cell glioblastoma; A2B5+)
Oligodendrocyte progenitor cells (OPC) isolated from high grade small cell glioblastoma (grade IV). OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen. Patients were 56 ± 3 years old males.
Control non-tumor oligodendrocyte progenitor cell sample (cortex)
Oligodendrocyte progenitor cells (OPC) isolated from cortical tissue, which was obtained from patients with epilepsy, but without any manifested brain cancer. OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen.

smoking study 80 (small airway epith; 3R4F; 48h; 0.16mg/l) / air exposed organotypic small airway epithelium culture sample (48h; 3R4F)

Relative Expression (log2-ratio):1.0655575
Number of Samples:6 / 6
Experimental smoking study 80 (small airway epith; 3R4F; 48h; 0.16mg/l)
Organotypic human small airway epithelium culture 48 hours after exposure to diluted mainstream cigarette smoke (0.16 mg nicotine/l) from 3R4F reference cigarettes. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The smoke was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each 3R4F cigarette was smoked to a defined standard butt length (approximately 35 mm), yielding approximately 10 – 11 puffs per stick. The cultures were exposed to smoke continuously for 28 minutes in smoking machine and 48 hours after smoke exposure the cells were collected for RNA extraction.
Control air exposed organotypic small airway epithelium culture sample (48h; 3R4F)
Organotypic human small airway epithelium culture 48 hours after exposure to conditioned fresh air. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The cultures were exposed to conditioned fresh air continuously for 28 minutes. These samples represented control samples for 3R4F smoke exposure at timepoint 48 hours after exposure.

glioma study 17 (glioblastoma; A2B5+) / non-tumor oligodendrocyte progenitor cell sample (cortex)

Relative Expression (log2-ratio):-1.0362234
Number of Samples:3 / 3
Experimental glioma study 17 (glioblastoma; A2B5+)
Oligodendrocyte progenitor cells (OPC) isolated from high grade glioblastoma (grade IV). OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen. Patients were 66 ± 5 years old males.
Control non-tumor oligodendrocyte progenitor cell sample (cortex)
Oligodendrocyte progenitor cells (OPC) isolated from cortical tissue, which was obtained from patients with epilepsy, but without any manifested brain cancer. OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen.

smoking study 80 (small airway epith; CHTP1.2; 48h; 0.15mg/l) / smoking study 80 (small airway epith; 3R4F; 48h; 0.16mg/l)

Relative Expression (log2-ratio):-1.0316772
Number of Samples:6 / 6
Experimental smoking study 80 (small airway epith; CHTP1.2; 48h; 0.15mg/l)
Organotypic human small airway epithelium culture 48 hours after exposure to 0.15 mg nicotine/l aerosol from a potential modified-risk tobacco product, carbon-heated tobacco product 1.2 (CHTP1.2). Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The aerosol was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each CHTP1.2 stick was used for a total of 12 puffs per stick. The cultures were exposed to aerosol continuously for 28 minutes in smoking machine and 48 hours after aerosol exposure the cells were collected for RNA extraction.
Control smoking study 80 (small airway epith; 3R4F; 48h; 0.16mg/l)
Organotypic human small airway epithelium culture 48 hours after exposure to diluted mainstream cigarette smoke (0.16 mg nicotine/l) from 3R4F reference cigarettes. Organotypic small airway SmallAirTM cultures were consisted of bronchiolar cells derived from a healthy non-smoker 55-year old female, fully differentiated and cultured at the air-liquid interface (37°C, 5% CO2, 90% humidity) and maintained in the respective culture medium in 12-well culture plates (0.7 ml/well), with a medium change every 2 – 3 days, for a maximum of 12 days before exposure. After exposure, the medium was not changed until it was collected. The smoke was generated according to the Health Canada Intense smoking protocol (a 55-mL puff over 2s, twice per minute with an 8-s pump exhaust time). Each 3R4F cigarette was smoked to a defined standard butt length (approximately 35 mm), yielding approximately 10 – 11 puffs per stick. The cultures were exposed to smoke continuously for 28 minutes in smoking machine and 48 hours after smoke exposure the cells were collected for RNA extraction.

influenza virus study 9 (A/pH1N1) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):-0.9974003
Number of Samples:3 / 3
Experimental influenza virus study 9 (A/pH1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype [A/Singapore/478/2009 (pH1N1)]. Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

lung cancer study 1 (PDX; pseudosarcomatous carcinoma; primary) / lung cancer study 1 (PDX; basaloid carcinoma; primary)

Relative Expression (log2-ratio):-0.9939327
Number of Samples:4 / 3
Experimental lung cancer study 1 (PDX; pseudosarcomatous carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary pseudosarcomatous carcinoma of the lung (subcutaneously implanted).
Control lung cancer study 1 (PDX; basaloid carcinoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary basaloid carcinoma of the lung (subcutaneously implanted).

influenza virus study 11 (A/H5N3) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):-0.983428
Number of Samples:3 / 3
Experimental influenza virus study 11 (A/H5N3)
Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F119/3/1997(H5N3). Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

influenza virus study 9 (A/H9N2) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):-0.9572058
Number of Samples:3 / 3
Experimental influenza virus study 9 (A/H9N2)
Human carcinoma cell line A549 infected with influenza A virus subtype A/Duck/Malaysia/01 (H9N2). Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.