TOP TEN perturbations for 1553868_a_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1553868_a_at
Selected probe(set): 233915_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1553868_a_at (233915_at) across 6674 perturbations tested by GENEVESTIGATOR:

systemic onset JIA study 6 (baseline; placebo) / normal blood sample

Relative Expression (log2-ratio):1.1349669
Number of Samples:22 / 22
Experimental systemic onset JIA study 6 (baseline; placebo)
Whole blood samples collected from systemic juvenile idiopathic arthritis patients at baseline (day 1), prior to placebo treatment. Patients were 2 to 19 years of age with a confirmed diagnosis of febrile SJIA with high disease activity. aACR response was scored at day 15. Concomitant therapy with a prednisone equivalent (up to 1.0 mg/kg/day) and stable doses of nonsteroidal anti inflammatory drugs and methotrexate (≤20 mg/m2 per week) were permitted.
Control normal blood sample
Whole blood samples collected from healthy volunteers.

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):1.103117
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

breast cancer study 14 / normal breast tissue

Relative Expression (log2-ratio):-0.79542255
Number of Samples:88 / 2
Experimental breast cancer study 14
Primary invasive ductal carcinoma tissue sample derived from the breasts of female patients.
Control normal breast tissue
Tissue samples of the breast from healthy female individuals.

asthma study 22 (eosinophilic AIP) / asthma study 22 (neutrophilic AIP)

Relative Expression (log2-ratio):-0.7395067
Number of Samples:15 / 17
Experimental asthma study 22 (eosinophilic AIP)
Sputum samples from asthmatic patients with eosinophilic airway inflammatory phenotype (AIP).
Control asthma study 22 (neutrophilic AIP)
Sputum samples from asthmatic patients with neutrophilic airway inflammatory phenotype (AIP).

HIF-1a depletion study 2 (normoxia; AB81) / control AB81 cell sample (normoxia)

Relative Expression (log2-ratio):0.6940341
Number of Samples:3 / 3
Experimental HIF-1a depletion study 2 (normoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with normoxia (20% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.
Control control AB81 cell sample (normoxia)
Human podocyte cell line AB81 treated with normoxia (20% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates.

nonspecific orbital inflammatory disease study 1 (lacrimal gland tissue) / normal lacrimal gland tissue

Relative Expression (log2-ratio):0.6827946
Number of Samples:42 / 7
Experimental nonspecific orbital inflammatory disease study 1 (lacrimal gland tissue)
Lacrimal gland tissue samples obtained from patients who suffered from nonspecific orbital inflammatory disease (NSOI). Biopsies were formalin-fixed and paraffin-embedded.
Control normal lacrimal gland tissue
Normal lacrimal gland tissue samples. The control tissue was obtained during surgery on eyes with non-inflamed orbits, such as blepharoplasties and enucleations. Biopsies were formalin-fixed and paraffin-embedded.

asthma study 22 (neutrophilic AIP) / asthma study 22 (paucigranulocytic AIP)

Relative Expression (log2-ratio):0.6709728
Number of Samples:17 / 10
Experimental asthma study 22 (neutrophilic AIP)
Sputum samples from asthmatic patients with neutrophilic airway inflammatory phenotype (AIP).
Control asthma study 22 (paucigranulocytic AIP)
Sputum samples from asthmatic patients with paucigranulocytic airway inflammatory phenotype (AIP).

rheumatoid arthritis study 37 (blood; post-treatment; TCZ; non-responder; IFX; non-responder) / rheumatoid arthritis study 37 (blood; naive)

Relative Expression (log2-ratio):-0.66302204
Number of Samples:4 / 18
Experimental rheumatoid arthritis study 37 (blood; post-treatment; TCZ; non-responder; IFX; non-responder)
Blood samples derived from patients with rheumatoid arthritis (RA) after treatment with tocilizumab (TCZ) and ifliximab (IFX) and classified as non-responder for both drugs. Samples were obtained at 24 weeks after the initiation of drug treatment. Patients who did not displayed a good response on drug treatment were classified as inadequate responders (non-responders) by European League Against Rheumatism (EULAR) response criteria at 24 weeks after the initiation of drug treatment. tocilizumab ATC code: infliximab ATC code:
Control rheumatoid arthritis study 37 (blood; naive)
Blood samples derived from drug-naive patients with rheumatoid arthritis (RA). Drug-naive patients were not being treated with moderate-to-high doses of corticosteroids, immunosuppressants, or biological agents when entered in to the study.

smoking study 56 (24 hrs) / smoking study 56 (baseline)

Relative Expression (log2-ratio):-0.6487999
Number of Samples:3 / 3
Experimental smoking study 56 (24 hrs)
Bronchial epithelium organotypic tissue culture harvested 24 hours (recovery phase) after 4 repetitive exposures to 10% (vol/vol) mainstream cigarette smoke (one 3R4F reference cigarette pro exposure), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts.
Control smoking study 56 (baseline)
Bronchial epithelium organotypic tissue culture harvested immediately after 4 repetitive exposures to 10% (vol/vol) mainstream cigarette smoke (one 3R4F reference cigarette pro exposure), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts.

sarcoidosis study 6 (orbital adipose tissue) / normal orbital adipose tissue

Relative Expression (log2-ratio):0.62763023
Number of Samples:7 / 22
Experimental sarcoidosis study 6 (orbital adipose tissue)
Orbital adipose tissue samples obtained from patients who suffered from sarcoidosis. Biopsies were formalin-fixed and paraffin-embedded.
Control normal orbital adipose tissue
Normal orbital adipose tissue samples. Samples were obtained from subjects with no history of orbital disease at the time of surgery such as fat typically excised and discarded as part of routine blepharoplasty or retrobulbar fat that is snared with the eye during enucleation. Biopsies were formalin-fixed and paraffin-embedded.