TOP TEN perturbations for 1554053_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1554053_at
Selected probe(set): 202277_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1554053_at (202277_at) across 6674 perturbations tested by GENEVESTIGATOR:

colorectal adenoma study 2 / normal colon tissue

Relative Expression (log2-ratio):-2.3770218
Number of Samples:5 / 6
Experimental colorectal adenoma study 2
Laser microdissected human adenoma sample.
Control normal colon tissue
Laser microdissected human colonic epithelial cells sample.

SPTLC123 study 1 (siRNA) / control siRNA transfected HuH-7 cell sample

Relative Expression (log2-ratio):-2.2563848
Number of Samples:3 / 3
Experimental SPTLC123 study 1 (siRNA)
Human HuH-7 hepatocarcinoma cell samples transfected with 20nM siRNA targeting SPTLC123 (serine transferase subunits 1, 2 and 3). Transfected cells were incubated for 24 hours at 37C, 5% CO2. The growth media (high-glucose, DMEM, 10% fetal bovine serum, 2 mM L-glutamine, 10 mM Hepes, and 1% penicillin-streptomycin) was replaced and cells were incubated for another 24 hours before the conditioned media (DMEM + 1% FBS + fatty acid free BSA) was added. Cells were incubated for another 24 hours prior to sampling.
Control control siRNA transfected HuH-7 cell sample
Human HuH-7 hepatocarcinoma cell samples transfected with 20nM scrambled, non-targeting siRNA. Transfected cells were incubated for 24 hours at 37C, 5% CO2. The growth media (high-glucose, DMEM, 10% fetal bovine serum, 2 mM L-glutamine, 10 mM Hepes, and 1% penicillin-streptomycin) was replaced and cells were incubated for another 24 hours before the conditioned media (DMEM + 1% FBS + fatty acid free BSA) was added. Cells were incubated for another 24 hours prior to sampling.

oncolytic herpes simplex virus study 2 / mock infected peripheral nerve sheath tumor (S462) cell sample

Relative Expression (log2-ratio):-2.2248068
Number of Samples:3 / 3
Experimental oncolytic herpes simplex virus study 2
Human malignant peripheral nerve sheath tumor (S462) cells infected with G207, an ICP34.5-deleted oncolytic herpes simplex virus (oHSV) for 6 hours.
Control mock infected peripheral nerve sheath tumor (S462) cell sample
Human malignant peripheral nerve sheath tumor (S462) cells mock infected for 6 hours.

DEGS1 study 1 (siRNA) / SPTLC123 study 1 (siRNA)

Relative Expression (log2-ratio):2.202773
Number of Samples:3 / 3
Experimental DEGS1 study 1 (siRNA)
Human HuH-7 hepatocarcinoma cell samples transfected with 20nM siRNA targeting DEGS1 (dihydroceramide desaturase). Transfected cells were incubated for 24 hours at 37C, 5% CO2. The growth media (high-glucose, DMEM, 10% fetal bovine serum, 2 mM L-glutamine, 10 mM Hepes, and 1% penicillin-streptomycin) was replaced and cells were incubated for another 24 hours before the conditioned media (DMEM + 1% FBS + fatty acid free BSA) was added. Cells were incubated for another 24 hours prior to sampling.
Control SPTLC123 study 1 (siRNA)
Human HuH-7 hepatocarcinoma cell samples transfected with 20nM siRNA targeting SPTLC123 (serine transferase subunits 1, 2 and 3). Transfected cells were incubated for 24 hours at 37C, 5% CO2. The growth media (high-glucose, DMEM, 10% fetal bovine serum, 2 mM L-glutamine, 10 mM Hepes, and 1% penicillin-streptomycin) was replaced and cells were incubated for another 24 hours before the conditioned media (DMEM + 1% FBS + fatty acid free BSA) was added. Cells were incubated for another 24 hours prior to sampling.

brefeldin A study 1 (0.5ug/ml; p53HCT116) / untreated p53HCT116 cell sample

Relative Expression (log2-ratio):2.1720896
Number of Samples:2 / 3
Experimental brefeldin A study 1 (0.5ug/ml; p53HCT116)
Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated p53HCT116 cell sample
Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

kidney transplantation study 11 (12 week) / normal blood sample

Relative Expression (log2-ratio):1.9390993
Number of Samples:10 / 5
Experimental kidney transplantation study 11 (12 week)
Whole blood samples derived from kidney transplant patients 12 weeks post-transplantation. Samples were collected 12 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal blood sample
Blood samples derived from healthy control subjects.

vMyb overexpr. study 1 (adenovirus) / control virus infected monocyte sample

Relative Expression (log2-ratio):-1.8993931
Number of Samples:2 / 2
Experimental vMyb overexpr. study 1 (adenovirus)
Approximately 1x10^6 monocytes were infected with adenovirus expressing v-Myb and GFP for 16 hours, then total RNA was isolated.
Control control virus infected monocyte sample
Approximately 1x10^6 monocytes were infected with control adenovirus expressing GFP for 16 hours, then total RNA was isolated.

myotonic dystrophy study 4 (deltoid muscle; DM-like) / normal skeletal muscle tisuue (fetal)

Relative Expression (log2-ratio):-1.8777161
Number of Samples:3 / 3
Experimental myotonic dystrophy study 4 (deltoid muscle; DM-like)
Deltoid muscle biopsies obtained from patients with myotonic-like dystrophy (DMx; DM-like; no DMPK or ZNF9 expansion).
Control normal skeletal muscle tisuue (fetal)
Commercially purchased normal fetal skeletal muscle tissue samples.

endometriosis study 6 (minimal/mild endo.; pro. MCP) / normal endometrium tissue (pro. MCP)

Relative Expression (log2-ratio):-1.8613176
Number of Samples:12 / 20
Experimental endometriosis study 6 (minimal/mild endo.; pro. MCP)
Endometrial tissue samples from women with minimal or mild endometriosis and pelvic pain and/or infertility collected in the proliferative menstrual cycle phase (MCP). Endometriosis was diagnosed based on the revised American Fertility Society classification system. The MCP was determined by endometrial histology, confirmed by estradiol and progesterone serum levels and corroborated by 2 independent bioinformatics methods: clustering in unsupervised whole-transcriptome principal component analysis and cycle phase assignment classifier analysis. Patients with hormonal treatment within previous 3 months and presence of malignancy or major systemic disease were excluded.
Control normal endometrium tissue (pro. MCP)
Normal endometrial tissue samples from women collected in the proliferative menstrual cycle phase (MCP). The MCP was determined by endometrial histology, confirmed by estradiol and progesterone serum levels and corroborated by 2 independent bioinformatics methods: clustering in unsupervised whole-transcriptome principal component analysis and cycle phase assignment classifier analysis.

brefeldin A study 1 (0.5ug/ml; HCT 116) / untreated HCT 116 cell sample

Relative Expression (log2-ratio):1.815402
Number of Samples:3 / 3
Experimental brefeldin A study 1 (0.5ug/ml; HCT 116)
Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 cell sample
Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.