TOP TEN perturbations for 1555600_s_at (Homo sapiens)

Organism: Homo sapiens
Gene: 1555600_s_at
Selected probe(set): 1555600_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 1555600_s_at (1555600_s_at) across 6674 perturbations tested by GENEVESTIGATOR:

IFN-g study 11 / unstimulated monocyte sample

Relative Expression (log2-ratio):4.1269455
Number of Samples:7 / 11
Experimental IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.
Control unstimulated monocyte sample
Monocyte samples isolated from healthy donors and incubated in whole blood for 1.5 hour without any stimulation. Following incubation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.

IFN-g study 3 / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):4.1009703
Number of Samples:2 / 17
Experimental IFN-g study 3
Bronchial epithelial cells (NHBE) treated with 100 ng/ml recombinant human interferon gamma (IFN-g; vendor: PeproTech / catalog number: 300-02 / catalog name: IFN-γ) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

IL-1b; IFN-g study 1 (108hrs) / untreated pancreatic islet sample (108hrs)

Relative Expression (log2-ratio):4.057439
Number of Samples:2 / 2
Experimental IL-1b; IFN-g study 1 (108hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 108 hours. Media were changed after 2 and 5 days.
Control untreated pancreatic islet sample (108hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 108 hours. Media were changed after 2 and 5 days.

IFN-g study 11 / normal monocyte sample

Relative Expression (log2-ratio):3.9067364
Number of Samples:7 / 7
Experimental IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.
Control normal monocyte sample
Monocyte samples isolated from healthy subjects. Peripheral blood monocytes were isolated immediately after drawing blood by depletion from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocyte by CD14 labeling.

rheumatoid arthritis study 31 / IFN-g study 11

Relative Expression (log2-ratio):-3.8831902
Number of Samples:4 / 7
Experimental rheumatoid arthritis study 31
Monocyte samples isolated from patients with rheumatoid arthritis (RA). Monocytes from whole blood were depleted from granulocytes by CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling. Patients fulfilled the revised American College of Rheumatology criteria (ACR) for RA.
Control IFN-g study 11
Monocyte samples isolated from donors and stimulated in vitro by 100 ng/ml IFNγ in whole blood for 1.5 hour. Following stimulation, monocytes were depleted from granulocytes using CD15 MACS beads, and afterwards they were sorted from remaining peripheral blood leukocytes by CD14 labeling.

IL-1b; IFN-g study 1 (24hrs) / untreated pancreatic islet sample (24hrs)

Relative Expression (log2-ratio):3.8628597
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (24hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 24 hours.
Control untreated pancreatic islet sample (24hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 24 hours.

TNF-ɑ; IL-1b; IL-6; PGE2 study 1 / control antibody treated immature dendritic cell sample

Relative Expression (log2-ratio):-3.809928
Number of Samples:4 / 5
Experimental TNF-ɑ; IL-1b; IL-6; PGE2 study 1
Immature dendritic cells (DCs) treated with inflammatory cytokine cocktail (10 ng/ml IL-1b, 1,000 U/ml IL-6, 10 ng/ml TNF-ɑ, 1μg/ml prostaglandin E2) for 24 h.
Control control antibody treated immature dendritic cell sample
Immature dendritic cells (DCs) treated with isotype control antibody (10 - 20μg/ml mouse IgG1 isotype) for 24 h.

IL-1b; IFN-g study 1 (8hrs) / untreated pancreatic islet sample (8hrs)

Relative Expression (log2-ratio):3.591631
Number of Samples:2 / 3
Experimental IL-1b; IFN-g study 1 (8hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 8 hours.
Control untreated pancreatic islet sample (8hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 8 hours.

IL-1b; IFN-g study 1 (12hrs) / untreated pancreatic islet sample (12hrs)

Relative Expression (log2-ratio):3.4631596
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (12hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 12 hours.
Control untreated pancreatic islet sample (12hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 12 hours.

IL-1b; IFN-g study 1 (36hrs) / untreated pancreatic islet sample (36hrs)

Relative Expression (log2-ratio):3.4235535
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (36hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 36 hours.
Control untreated pancreatic islet sample (36hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 36 hours.