TOP TEN perturbations for 200900_s_at (Homo sapiens)

Organism: Homo sapiens
Gene: 200900_s_at
Selected probe(set): 200901_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 200900_s_at (200901_s_at) across 5638 perturbations tested by GENEVESTIGATOR:

oncolytic herpes simplex virus study 2 / mock infected peripheral nerve sheath tumor (S462) cell sample

Relative Expression (log2-ratio):-2.6237803
Number of Samples:3 / 3
Experimental oncolytic herpes simplex virus study 2
Human malignant peripheral nerve sheath tumor (S462) cells infected with G207, an ICP34.5-deleted oncolytic herpes simplex virus (oHSV) for 6 hours.
Control mock infected peripheral nerve sheath tumor (S462) cell sample
Human malignant peripheral nerve sheath tumor (S462) cells mock infected for 6 hours.

T-cell isolation study 11 / T-cell isolation study 6

Relative Expression (log2-ratio):-2.3609085
Number of Samples:2 / 2
Experimental T-cell isolation study 11
CD4+ resting memory T-cell were isolated from peripheral blood buffy coat of healthy donors after storage for 24 hours at 20°C. The cell fraction was first enriched via density gradient centrifugation with LSM 1077 Ficoll and than FACS sorted as CD3+/CD4+/CD45RO+/CD45RA- population.
Control T-cell isolation study 6
CD4+ resting memory T-cell were isolated from whole peripheral blood of healthy donors with no delay. The cell fraction was first enriched via density gradient centrifugation with LSM 1077 Ficoll and than FACS sorted as CD3+/CD4+/CD45RO+/CD45RA- population.

stem cell differentiation study 41 (hES-T3 EB) / undifferentiated hES-T3 cell sample

Relative Expression (log2-ratio):2.1219482
Number of Samples:2 / 3
Experimental stem cell differentiation study 41 (hES-T3 EB)
Sample of embryonic bodies (EB) differentiated from human embryonic stem cells T3 with female karyotype.
Control undifferentiated hES-T3 cell sample
Undifferentiated human embryonic stem cells T3.

IgG; C3bi stimulation study 1 (early) / normal polymorphnuclear leukocyte sample

Relative Expression (log2-ratio):2.0263796
Number of Samples:11 / 10
Experimental IgG; C3bi stimulation study 1 (early)
Polymorphonuclear leukocytes (PMNs) from healthy donors, incubated with IgG- and C3bi-coated latex beads at 37°C in a CO2 incubator for 3h. ATC code:---
Control normal polymorphnuclear leukocyte sample
Polymorphonuclear leukocytes (PMNs) from healthy donors, incubated without IgG- and C3bi-coated latex beads at 37°C in a CO2 incubator for 3h.

B-CLL study 11 (rolipram) / rolipram study 4 (normal B-cell; 20uM)

Relative Expression (log2-ratio):1.9455385
Number of Samples:4 / 4
Experimental B-CLL study 11 (rolipram)
Peripheral blood mononuclear cells (PBMCs) obtained from chronic lymphocytic leukemia (CLL) patients and in vitro treated with rolipram (20 uM, cyclic nucleotide phosphodiesterase (PDE4) inhibitor) for 4 hours. PBMCs’ samples contained 90% CD19+ CD5+ B lineage CLL cells (B-CLL). Inclusion criteria: untreated CLL patients or at least 1 month after chemotherapy. Exclusion criteria: patients with active infections or other serious medical conditions or with white blood cell (WBC) counts of 15,000/l. ATC code:---
Control rolipram study 4 (normal B-cell; 20uM)
MACS purified resting B-cells from healthy donor peripheral blood treated with rolipram (20 uM, cyclic nucleotide phosphodiesterase (PDE4) inhibitor) for 4 hours. ATC code:---

IgG; C3bi stimulation study 2 (late) / untreated polymorphnuclear leukocyte sample (Job's syndrome)

Relative Expression (log2-ratio):1.796525
Number of Samples:7 / 6
Experimental IgG; C3bi stimulation study 2 (late)
Polymorphonuclear leukocytes (PMNs) from Job's syndrome patients, incubated with IgG- and C3bi-coated latex beads at 37°C in a CO2 incubator for 6h. ATC code:---
Control untreated polymorphnuclear leukocyte sample (Job's syndrome)
Polymorphonuclear leukocytes (PMNs) from Job's syndrome patients, incubated without IgG- and C3bi-coated latex beads at 37°C in a CO2 incubator for 6h.

B-CLL study 11 (DMSO) / vehicle (DMSO) treated normal B-cell sample

Relative Expression (log2-ratio):1.7771502
Number of Samples:4 / 4
Experimental B-CLL study 11 (DMSO)
Peripheral blood mononuclear cells (PBMCs) obtained from chronic lymphocytic leukemia (CLL) patients and in vitro treated with vehicle (DMSO) for 4 hours. PBMCs’ samples contained 90% CD19+ CD5+ B lineage CLL cells (B-CLL). Inclusion criteria: untreated CLL patients or at least 1 month after chemotherapy. Exclusion criteria: patients with active infections or other serious medical conditions or with white blood cell (WBC) counts of 15,000/l.
Control vehicle (DMSO) treated normal B-cell sample
MACS purified resting B-cells from healthy donor peripheral blood treated with vehicle (DMSO) for 4 hours.

conditioned medium study 1 (HS27a) / untreated monocyte (CD14+) sample

Relative Expression (log2-ratio):1.7701569
Number of Samples:2 / 2
Experimental conditioned medium study 1 (HS27a)
CD14+ monocytes treated with HS27a conditioned medium (CM) for 48h.
Control untreated monocyte (CD14+) sample
Untreated CD14+ monocytes from two different donors.

stem cell differentiation study 41 (T3pi) / undifferentiated hES-T3 cell sample

Relative Expression (log2-ratio):1.7175102
Number of Samples:2 / 3
Experimental stem cell differentiation study 41 (T3pi)
Sample of pancreatic islet-like cell clusters differentiated from human embryonic stem cells T3 with female karyotype.
Control undifferentiated hES-T3 cell sample
Undifferentiated human embryonic stem cells T3.

IgG; C3bi stimulation study 1 (late) / normal polymorphnuclear leukocyte sample

Relative Expression (log2-ratio):1.636157
Number of Samples:10 / 11
Experimental IgG; C3bi stimulation study 1 (late)
Polymorphonuclear leukocytes (PMNs) from healthy donors, incubated with IgG- and C3bi-coated latex beads at 37°C in a CO2 incubator for 6h. ATC code:---
Control normal polymorphnuclear leukocyte sample
Polymorphonuclear leukocytes (PMNs) from healthy donors, incubated without IgG- and C3bi-coated latex beads at 37°C in a CO2 incubator for 6h.