TOP TEN perturbations for 38388_at (Homo sapiens)

Organism: Homo sapiens
Gene: 38388_at
Selected probe(set): 202869_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 38388_at (202869_at) across 6674 perturbations tested by GENEVESTIGATOR:

T. cruzi study 1 (VSMC; 48h) / mock infected vascular smooth muscle cell sample (48h)

Relative Expression (log2-ratio):6.9647293
Number of Samples:3 / 3
Experimental T. cruzi study 1 (VSMC; 48h)
Human vascular smooth muscle cells (VSMCs) infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 48 hours post infection. Cells were maintained in Ham’s F12K medium with 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 10 mM HEPES, 10 mM TES, 0.05 mg/ml ascorbic acid, 0.001 mg/ml insulin, 0.01 mg/ml transferrin, 10ng/ml sodium selenite, 0.03 mg/ml endothelial cell growth supplement (ECGS) and 10% FBS.
Control mock infected vascular smooth muscle cell sample (48h)
Human vascular smooth muscle cells (VSMCs) mock-infected and harvested 48 hours post mock infection with 2% FBS medium. Cells were maintained in Ham’s F12K medium with 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 10 mM HEPES, 10 mM TES, 0.05 mg/ml ascorbic acid, 0.001 mg/ml insulin, 0.01 mg/ml transferrin, 10ng/ml sodium selenite, 0.03 mg/ml endothelial cell growth supplement (ECGS) and 10% FBS.

T. cruzi study 1 (BJ; 24h; top) / mock infected foreskin fibroblast (BJ) sample (24h; top)

Relative Expression (log2-ratio):6.8677254
Number of Samples:2 / 2
Experimental T. cruzi study 1 (BJ; 24h; top)
Foreskin fibroblast (BJ) cells grown in the transwell top, bathed with media from the BJ cells infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 24 hours post infection. In the transwell setup, cells in the top chamber served as reporters for the effect of all difusible factors secreted by parasite-infected cells in the bottom chamber. Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin.
Control mock infected foreskin fibroblast (BJ) sample (24h; top)
Foreskin fibroblast (BJ) cells grown in the transwell top, bathed with media from the mock-infected bottom BJ cells, harvested 24 hours post mock infection with 2% FBS medium. In the transwell setup, cells in the top chamber served as reporters for the effect of all difusible factors secreted by mock-infected cells in the bottom chamber. Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin.

T. cruzi study 1 (HMVEC; 24h; top) / mock infected cardiac microvascular endothelial cell sample (24h; top)

Relative Expression (log2-ratio):6.7219934
Number of Samples:2 / 2
Experimental T. cruzi study 1 (HMVEC; 24h; top)
Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell top, bathed with media from the HMVECs infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 24 hours post infection. In the transwell setup, cells in the top chamber served as reporters for the effect of all difusible factors secreted by parasite-infected cells in the bottom chamber. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS.
Control mock infected cardiac microvascular endothelial cell sample (24h; top)
Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell top, bathed with media from the mock-infected bottom HMVECs, harvested 24 hours post mock infection with 2% FBS medium. In the transwell setup, cells in the top chamber served as reporters for the effect of all difusible factors secreted by mock-infected cells in the bottom chamber. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS.

T. cruzi study 1 (BJ; 24h; bottom) / mock infected foreskin fibroblast (BJ) sample (24h; bottom)

Relative Expression (log2-ratio):6.710966
Number of Samples:2 / 2
Experimental T. cruzi study 1 (BJ; 24h; bottom)
Foreskin fibroblast (BJ) cells grown in the transwell bottom, infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 24 hours post infection. Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin.
Control mock infected foreskin fibroblast (BJ) sample (24h; bottom)
Foreskin fibroblast (BJ) cells grown in the transwell bottom, mock-infected and harvested 24 hours post mock infection with 2% FBS medium. Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin.

glioma study 16 (LN-319) / normal astrocyte sample

Relative Expression (log2-ratio):6.3002615
Number of Samples:2 / 3
Experimental glioma study 16 (LN-319)
Human glioma cell line LN319 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

T. cruzi study 1 (HMVEC; 24h; bottom) / mock infected cardiac microvascular endothelial cell sample (24h; bottom)

Relative Expression (log2-ratio):6.2659225
Number of Samples:2 / 2
Experimental T. cruzi study 1 (HMVEC; 24h; bottom)
Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell bottom, infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 24 hours post infection. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS.
Control mock infected cardiac microvascular endothelial cell sample (24h; bottom)
Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell bottom, mock-infected and harvested 24 hours post mock infection with 2% FBS medium. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS.

interferon-alpha-A/D study 1 (early) / untreated BE(2)-C cell sample (mature)

Relative Expression (log2-ratio):5.5899315
Number of Samples:3 / 3
Experimental interferon-alpha-A/D study 1 (early)
Cultured BE(2)-C cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics and incubated with universal type I IFN (IFN-alpha-A/D) for 6 hours before samples were taken. ATC code:---
Control untreated BE(2)-C cell sample (mature)
Differentiated, cultured BE(2)-C neuroblastoma cells. Cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics before samples were taken.

IFN-a2b study 2 / untreated mouse-derived ovarian cancer cell (sidep.) sample

Relative Expression (log2-ratio):5.56526
Number of Samples:2 / 2
Experimental IFN-a2b study 2
Side population subset of ovarian cancer cells derived from the malignant effusion developed in mice injected with cells obtained from ascitic fluid of ovarian cancer bearing-patients. Cells were treated with 1000 IU/ml IFN-alpha2b for 5 hours.
Control untreated mouse-derived ovarian cancer cell (sidep.) sample
Side population subset of ovarian cancer cells derived from the malignant effusion developed in mice injected with cells obtained from ascitic fluid of ovarian cancer bearing-patients. Cells were untreated.

smoking study 81 (CSE) / human rhinovirus study 3

Relative Expression (log2-ratio):-5.513216
Number of Samples:4 / 4
Experimental smoking study 81 (CSE)
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to a treatment with 50% cigarette smoke extract (CSE) for 24 hours. In order to prepare CSE, one reference cigarette 3R4F was bubbled into 4 ml of hydrocortisone-free bronchial epithelial cell growth medium (BEGM) for 5 minutes using a syringe apparatus. Absorbance of the obtained CSE was adjusted to 0.15 at 320 nm and the resulting CSE was used at 50% concentration. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the CSE stimulation. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.
Control human rhinovirus study 3
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to an infection with human rhinovirus 16 (HRV-16; MOI=1) for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the HRV-16 infection. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.

polyinosinic-polycytidylic acid study 1 (10ug/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):5.506736
Number of Samples:3 / 17
Experimental polyinosinic-polycytidylic acid study 1 (10ug/ml)
Bronchial epithelial cells (NHBE) treated with polyinosinic-polycytidylic acid (Poly(I:C); 10ug/ml; vendor: InvivoGen / catalog number: tlrl-pic / catalog name: Poly(I:C) High Molecular Weight [31852-29-6]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).