TOP TEN perturbations for 38419_at (Homo sapiens)

Organism: Homo sapiens
Gene: 38419_at
Selected probe(set): 201985_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 38419_at (201985_at) across 6674 perturbations tested by GENEVESTIGATOR:

esophagus cancer study 1 (PDX; squamous cell carcinoma, NOS; metastatic) / esophagus cancer study 1 (PDX; squamous cell carcinoma, NOS; primary)

Relative Expression (log2-ratio):-2.9291267
Number of Samples:2 / 3
Experimental esophagus cancer study 1 (PDX; squamous cell carcinoma, NOS; metastatic)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from a metastasis of patients with primary squamous cell carcinoma, NOS of the oesophagus (subcutaneously implanted). Metastatic site of patient tumor sample is not reported.
Control esophagus cancer study 1 (PDX; squamous cell carcinoma, NOS; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary squamous cell carcinoma, NOS of the oesophagus (subcutaneously implanted).

bone cancer study 1 (PDX; chondroblastic osteosarcoma; primary) / bone cancer study 1 (PDX; osteosarcoma, NOS; primary)

Relative Expression (log2-ratio):2.7748852
Number of Samples:2 / 2
Experimental bone cancer study 1 (PDX; chondroblastic osteosarcoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary chondroblastic osteosarcoma of the bone (subcutaneously implanted).
Control bone cancer study 1 (PDX; osteosarcoma, NOS; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary osteosarcoma, NOS of the bone (subcutaneously implanted).

IgG; C3bi stimulation study 1 (early) / normal polymorphnuclear leukocyte sample

Relative Expression (log2-ratio):2.2942352
Number of Samples:11 / 10
Experimental IgG; C3bi stimulation study 1 (early)
Polymorphonuclear leukocytes (PMNs) from healthy donors, incubated with IgG- and C3bi-coated latex beads at 37°C in a CO2 incubator for 3h. ATC code:---
Control normal polymorphnuclear leukocyte sample
Polymorphonuclear leukocytes (PMNs) from healthy donors, incubated without IgG- and C3bi-coated latex beads at 37°C in a CO2 incubator for 3h.

P. aeruginosa study 3 (∆F508-CFTR compl) / P. aeruginosa study 3 (wt-CFTR compl)

Relative Expression (log2-ratio):-2.235938
Number of Samples:4 / 4
Experimental P. aeruginosa study 3 (∆F508-CFTR compl)
Human bronchial epithelial cell line homozygous for the cystic fibrosis mutation F508-CFTR and complemented with ∆F508-CFTR infected for 1 hour with P. aeruginosa stain PAO1 (MOI 30:1). Cells were seeded on Trans-well filters and grown in air-liquid interface for 8 days to develop polarized monolayers. P. aeruginosa was applied to the cells by removing the medium from the apical side and replacing it with equivalent volume of antibiotics-free medium containing bacteria. After 1h stimulation cells were washed twice in medium and mRNA isolated five hours later.
Control P. aeruginosa study 3 (wt-CFTR compl)
Human bronchial epithelial cell line homozygous for the cystic fibrosis mutation F508-CFTR and complemented with wt-CFTR infected for 1 hour with P. aeruginosa stain PAO1 (MOI 30:1). Cells were seeded on Trans-well filters and grown in air-liquid interface for 8 days to develop polarized monolayers. P. aeruginosa was applied to the cells by removing the medium from the apical side and replacing it with equivalent volume of antibiotics-free medium containing bacteria. After 1h stimulation cells were washed twice in medium and mRNA isolated five hours later.

CAR T cell study 4 (PSCA-28t28Z; post-infusion) / CAR T cell study 4 (PSCA-28t28Z; pre-infusion)

Relative Expression (log2-ratio):2.0939407
Number of Samples:3 / 3
Experimental CAR T cell study 4 (PSCA-28t28Z; post-infusion)
CD8+ T cells transduced with PSCA-28t28Z (second generation CAR) and isolated 30 days after adoptive transfer into mice bearing HPAC-derived pancreatic tumor. Human peripheral blood mononuclear cells were stimulated with anti-CD3 antibody (OKT3) in presence of IL-2. Two days post-stimulation, T cells were transduced with retroviral vectors encoding PSCA-28t28Z. Cells were cultured for 2 weeks in presence of IL-2 and then transfered into 4-5-week-old male NSG mice. Subcutaneous xenografts were generated by injection of HPAC cells. Once tumors became palpable, mice were treated with CD8+ T cells expressing PSCA-28t28Z. Untransduced CD4+ cells from the same donor were given to each mouse for cytokine support. Spleen-resident human CD8+ T cells were isolated 30 days later using the CD8 MicroBeads (post-infusion samples).
Control CAR T cell study 4 (PSCA-28t28Z; pre-infusion)
Primary human CD8+ T cells stimulated ex vivo and transduced to express PSCA-28t28Z (second generation CAR). Human peripheral blood mononuclear cells were stimulated with anti-CD3 antibody (OKT3) in presence of IL-2. Two days post-stimulation, T cells were transduced with retroviral vectors encoding PSCA-28t28Z. Cells were cultured for 2 weeks in presence of IL-2, until collection of samples (pre-infusion samples).

CAR T cell study 4 (PSCA-28t28Z; pre-infusion) / CAR T cell study 4 (GFP; pre-infusion)

Relative Expression (log2-ratio):-2.0797825
Number of Samples:3 / 3
Experimental CAR T cell study 4 (PSCA-28t28Z; pre-infusion)
Primary human CD8+ T cells stimulated ex vivo and transduced to express PSCA-28t28Z (second generation CAR). Human peripheral blood mononuclear cells were stimulated with anti-CD3 antibody (OKT3) in presence of IL-2. Two days post-stimulation, T cells were transduced with retroviral vectors encoding PSCA-28t28Z. Cells were cultured for 2 weeks in presence of IL-2, until collection of samples (pre-infusion samples).
Control CAR T cell study 4 (GFP; pre-infusion)
Primary human CD8+ T cells stimulated ex vivo and transduced to express GFP. Human peripheral blood mononuclear cells were stimulated with anti-CD3 antibody (OKT3) in presence of IL-2. Two days post-stimulation, T cells were transduced with retroviral vectors encoding GFP as a control. Cells were cultured for 2 weeks in presence of IL-2, until collection of samples (pre-infusion samples).

cystic fibrosis study 4 (∆F508-CFTR compl) / cystic fibrosis study 4 (wt-CFTR compl)

Relative Expression (log2-ratio):-2.063858
Number of Samples:4 / 4
Experimental cystic fibrosis study 4 (∆F508-CFTR compl)
Uninfected human bronchial epithelial cell line homozygous for the cystic fibrosis mutation F508-CFTR (Cystic fibrosis transmembrane conductance regulator) and complemented with ∆F508-CFTR. Cells were seeded on Trans-well filters and grown in air-liquid interface for 8 days to develop polarized monolayers. Vehicle was applied to the cells by removing the medium from the apical side and replacing it with equivalent volume of antibiotics-free medium. After 1h vehicle treatment cells were washed twice in medium and mRNA isolated five hours later.
Control cystic fibrosis study 4 (wt-CFTR compl)
Uninfected human bronchial epithelial cell line homozygous for the cystic fibrosis mutation F508-CFTR (Cystic fibrosis transmembrane conductance regulator) and complemented with wt-CFTR. Cells were seeded on Trans-well filters and grown in air-liquid interface for 8 days to develop polarized monolayers. Vehicle was applied to the cells by removing the medium from the apical side and replacing it with equivalent volume of antibiotics-free medium. After 1h vehicle treatment cells were washed twice in medium and mRNA isolated five hours later.

tunicamycin study 2 (2ug/ml; HCT 116) / untreated HCT 116 cell sample

Relative Expression (log2-ratio):1.9775925
Number of Samples:3 / 3
Experimental tunicamycin study 2 (2ug/ml; HCT 116)
Human colon carcinoma cell line HCT116 was treated with 2 ug/ml tunicamycin for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 cell sample
Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

F. tularensis study 1 (novicida) / uninfected peripheral blood monocyte sample

Relative Expression (log2-ratio):-1.9022388
Number of Samples:4 / 6
Experimental F. tularensis study 1 (novicida)
Peripheral blood monocytes infected with the Francisella tularensis subspecies novicida isolate U112 (100 MOI) for 24 hours.
Control uninfected peripheral blood monocyte sample
Peripheral blood monocytes uninfected.

bone cancer study 1 (PDX; chondroblastic osteosarcoma; primary) / bone cancer study 1 (PDX; myxoid chondrosarcoma; primary)

Relative Expression (log2-ratio):1.8778839
Number of Samples:2 / 2
Experimental bone cancer study 1 (PDX; chondroblastic osteosarcoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary chondroblastic osteosarcoma of the bone (subcutaneously implanted).
Control bone cancer study 1 (PDX; myxoid chondrosarcoma; primary)
Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary myxoid chondrosarcoma of the bone (subcutaneously implanted).