TOP TEN perturbations for 384_at (Homo sapiens)
Organism: Homo sapiens
Gene: 384_at
Selected probe(set): 202659_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 384_at (202659_at) across 6674 perturbations tested by GENEVESTIGATOR:
IFN-g study 9 (24h) / untreated epidermal keratinocyte sample
Relative Expression (log2-ratio):3.2674809Number of Samples:3 / 6
| Experimental | IFN-g study 9 (24h) |
| Primary keratinocytes treated with interferon gamma (IFN-g) for 24 hours. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency. Cultures were starved of growth factors in nonsupplemented M154 medium for 24 hours, and afterwards stimulated with recombinant human IFN-g for 24 hours. | |
| Control | untreated epidermal keratinocyte sample |
| Untreated primary epidermal keratinocytes. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency. |
expO breast cancer study 1 (papillary carcinoma, NOS; primary) / expO breast cancer study 1 (medullary carcinoma, NOS; primary)
Relative Expression (log2-ratio):-2.7448282Number of Samples:2 / 2
| Experimental | expO breast cancer study 1 (papillary carcinoma, NOS; primary) |
| Primary tumor tissue samples obtained from the breast of patients with papillary carcinoma (NOS). | |
| Control | expO breast cancer study 1 (medullary carcinoma, NOS; primary) |
| Primary tumor tissue samples obtained from the breast of patients with medullary carcinoma (NOS). |
IL-1b; IFN-g study 1 (36hrs) / untreated pancreatic islet sample (36hrs)
Relative Expression (log2-ratio):2.6971855Number of Samples:3 / 3
| Experimental | IL-1b; IFN-g study 1 (36hrs) |
| Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 36 hours. | |
| Control | untreated pancreatic islet sample (36hrs) |
| Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 36 hours. |
IFN-g study 3 / vehicle (DME) treated bronchial epithelial cell sample
Relative Expression (log2-ratio):2.6844082Number of Samples:2 / 17
| Experimental | IFN-g study 3 |
| Bronchial epithelial cells (NHBE) treated with 100 ng/ml recombinant human interferon gamma (IFN-g; vendor: PeproTech / catalog number: 300-02 / catalog name: IFN-γ) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). | |
| Control | vehicle (DME) treated bronchial epithelial cell sample |
| Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). |
expO ovary cancer study 1 (granulosa cell tumor, malignant; metastatic) / expO ovary cancer study 1 (dysgerminoma; metastatic)
Relative Expression (log2-ratio):-2.6626081Number of Samples:2 / 2
| Experimental | expO ovary cancer study 1 (granulosa cell tumor, malignant; metastatic) |
| Metastatic tumor tissue samples obtained from patients with primary granulosa cell tumor of the ovary. | |
| Control | expO ovary cancer study 1 (dysgerminoma; metastatic) |
| Metastatic tumor tissue samples obtained from patients with primary dysgerminoma of the ovary. |
TNF-ɑ; TGF-ß2 study 1 (intermediate) / untreated ARPE-19 cell sample
Relative Expression (log2-ratio):2.6067295Number of Samples:6 / 3
| Experimental | TNF-ɑ; TGF-ß2 study 1 (intermediate) |
| ARPE-19 retina pigment epithelial cell samples treated with TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml). Samples were taken 16 and 24 hours after treatment. | |
| Control | untreated ARPE-19 cell sample |
| Untreated ARPE-19 retina pigment epithelial cell samples harvested before adding TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml). |
Treg activation study 1 (300min) / unstimulated regulatory T-cell sample
Relative Expression (log2-ratio):-2.589182Number of Samples:2 / 2
| Experimental | Treg activation study 1 (300min) |
| Regulatory T-cells were stimulated for 300min with anti-CD3/anti-CD28/IL-2 (100U/ml). Treg were sorted as CD4+ CD25high cells from peripheral blood of healthy donors. | |
| Control | unstimulated regulatory T-cell sample |
| Unstimulated regulatory T-cell sample derived from sorted CD4+ CD25high cells from peripheral blood of healthy donors. |
IL-1b; IFN-g study 1 (72hrs) / untreated pancreatic islet sample (72hrs)
Relative Expression (log2-ratio):2.5796957Number of Samples:3 / 3
| Experimental | IL-1b; IFN-g study 1 (72hrs) |
| Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 72 hours. Media were changed after 2 days. | |
| Control | untreated pancreatic islet sample (72hrs) |
| Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 72 hours. Media were changed after 2 days. |
IL-1b; IFN-g study 1 (132hrs) / untreated pancreatic islet sample (132hrs)
Relative Expression (log2-ratio):2.5747051Number of Samples:2 / 2
| Experimental | IL-1b; IFN-g study 1 (132hrs) |
| Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 132 hours. Media were changed after 2 and 5 days. | |
| Control | untreated pancreatic islet sample (132hrs) |
| Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 132 hours. Media were changed after 2 and 5 days. |
IL-1b; IFN-g study 1 (96hrs) / untreated pancreatic islet sample (96hrs)
Relative Expression (log2-ratio):2.5681248Number of Samples:3 / 3
| Experimental | IL-1b; IFN-g study 1 (96hrs) |
| Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 96 hours. Media were changed after 2 days. | |
| Control | untreated pancreatic islet sample (96hrs) |
| Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 96 hours. Media were changed after 2 days. |
Organism: Homo sapiens
Gene: 384_at
Selected probe(set): 202659_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 384_at (202659_at) across 6674 perturbations tested by GENEVESTIGATOR:
IFN-g study 9 (24h) / untreated epidermal keratinocyte sample
Relative Expression (log2-ratio):3.2674809Number of Samples:3 / 6
| Experimental | IFN-g study 9 (24h) |
| Primary keratinocytes treated with interferon gamma (IFN-g) for 24 hours. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency. Cultures were starved of growth factors in nonsupplemented M154 medium for 24 hours, and afterwards stimulated with recombinant human IFN-g for 24 hours. | |
| Control | untreated epidermal keratinocyte sample |
| Untreated primary epidermal keratinocytes. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency. |
expO breast cancer study 1 (papillary carcinoma, NOS; primary) / expO breast cancer study 1 (medullary carcinoma, NOS; primary)
Relative Expression (log2-ratio):-2.7448282Number of Samples:2 / 2
| Experimental | expO breast cancer study 1 (papillary carcinoma, NOS; primary) |
| Primary tumor tissue samples obtained from the breast of patients with papillary carcinoma (NOS). | |
| Control | expO breast cancer study 1 (medullary carcinoma, NOS; primary) |
| Primary tumor tissue samples obtained from the breast of patients with medullary carcinoma (NOS). |
IL-1b; IFN-g study 1 (36hrs) / untreated pancreatic islet sample (36hrs)
Relative Expression (log2-ratio):2.6971855Number of Samples:3 / 3
| Experimental | IL-1b; IFN-g study 1 (36hrs) |
| Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 36 hours. | |
| Control | untreated pancreatic islet sample (36hrs) |
| Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 36 hours. |
IFN-g study 3 / vehicle (DME) treated bronchial epithelial cell sample
Relative Expression (log2-ratio):2.6844082Number of Samples:2 / 17
| Experimental | IFN-g study 3 |
| Bronchial epithelial cells (NHBE) treated with 100 ng/ml recombinant human interferon gamma (IFN-g; vendor: PeproTech / catalog number: 300-02 / catalog name: IFN-γ) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). | |
| Control | vehicle (DME) treated bronchial epithelial cell sample |
| Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). |
expO ovary cancer study 1 (granulosa cell tumor, malignant; metastatic) / expO ovary cancer study 1 (dysgerminoma; metastatic)
Relative Expression (log2-ratio):-2.6626081Number of Samples:2 / 2
| Experimental | expO ovary cancer study 1 (granulosa cell tumor, malignant; metastatic) |
| Metastatic tumor tissue samples obtained from patients with primary granulosa cell tumor of the ovary. | |
| Control | expO ovary cancer study 1 (dysgerminoma; metastatic) |
| Metastatic tumor tissue samples obtained from patients with primary dysgerminoma of the ovary. |
TNF-ɑ; TGF-ß2 study 1 (intermediate) / untreated ARPE-19 cell sample
Relative Expression (log2-ratio):2.6067295Number of Samples:6 / 3
| Experimental | TNF-ɑ; TGF-ß2 study 1 (intermediate) |
| ARPE-19 retina pigment epithelial cell samples treated with TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml). Samples were taken 16 and 24 hours after treatment. | |
| Control | untreated ARPE-19 cell sample |
| Untreated ARPE-19 retina pigment epithelial cell samples harvested before adding TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml). |
Treg activation study 1 (300min) / unstimulated regulatory T-cell sample
Relative Expression (log2-ratio):-2.589182Number of Samples:2 / 2
| Experimental | Treg activation study 1 (300min) |
| Regulatory T-cells were stimulated for 300min with anti-CD3/anti-CD28/IL-2 (100U/ml). Treg were sorted as CD4+ CD25high cells from peripheral blood of healthy donors. | |
| Control | unstimulated regulatory T-cell sample |
| Unstimulated regulatory T-cell sample derived from sorted CD4+ CD25high cells from peripheral blood of healthy donors. |
IL-1b; IFN-g study 1 (72hrs) / untreated pancreatic islet sample (72hrs)
Relative Expression (log2-ratio):2.5796957Number of Samples:3 / 3
| Experimental | IL-1b; IFN-g study 1 (72hrs) |
| Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 72 hours. Media were changed after 2 days. | |
| Control | untreated pancreatic islet sample (72hrs) |
| Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 72 hours. Media were changed after 2 days. |
IL-1b; IFN-g study 1 (132hrs) / untreated pancreatic islet sample (132hrs)
Relative Expression (log2-ratio):2.5747051Number of Samples:2 / 2
| Experimental | IL-1b; IFN-g study 1 (132hrs) |
| Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 132 hours. Media were changed after 2 and 5 days. | |
| Control | untreated pancreatic islet sample (132hrs) |
| Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 132 hours. Media were changed after 2 and 5 days. |
IL-1b; IFN-g study 1 (96hrs) / untreated pancreatic islet sample (96hrs)
Relative Expression (log2-ratio):2.5681248Number of Samples:3 / 3
| Experimental | IL-1b; IFN-g study 1 (96hrs) |
| Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 96 hours. Media were changed after 2 days. | |
| Control | untreated pancreatic islet sample (96hrs) |
| Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 96 hours. Media were changed after 2 days. |