TOP TEN perturbations for 38584_at (Homo sapiens)

Organism: Homo sapiens
Gene: 38584_at
Selected probe(set): 229450_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 38584_at (229450_at) across 6674 perturbations tested by GENEVESTIGATOR:

smoking study 81 (CSE) / human rhinovirus study 3

Relative Expression (log2-ratio):-8.683232
Number of Samples:4 / 4
Experimental smoking study 81 (CSE)
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to a treatment with 50% cigarette smoke extract (CSE) for 24 hours. In order to prepare CSE, one reference cigarette 3R4F was bubbled into 4 ml of hydrocortisone-free bronchial epithelial cell growth medium (BEGM) for 5 minutes using a syringe apparatus. Absorbance of the obtained CSE was adjusted to 0.15 at 320 nm and the resulting CSE was used at 50% concentration. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the CSE stimulation. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.
Control human rhinovirus study 3
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to an infection with human rhinovirus 16 (HRV-16; MOI=1) for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the HRV-16 infection. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.

T. cruzi study 1 (HMVEC; 24h; bottom) / mock infected cardiac microvascular endothelial cell sample (24h; bottom)

Relative Expression (log2-ratio):8.588274
Number of Samples:2 / 2
Experimental T. cruzi study 1 (HMVEC; 24h; bottom)
Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell bottom, infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 24 hours post infection. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS.
Control mock infected cardiac microvascular endothelial cell sample (24h; bottom)
Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell bottom, mock-infected and harvested 24 hours post mock infection with 2% FBS medium. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS.

T. cruzi study 1 (HMVEC; 24h; top) / mock infected cardiac microvascular endothelial cell sample (24h; top)

Relative Expression (log2-ratio):8.583575
Number of Samples:2 / 2
Experimental T. cruzi study 1 (HMVEC; 24h; top)
Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell top, bathed with media from the HMVECs infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 24 hours post infection. In the transwell setup, cells in the top chamber served as reporters for the effect of all difusible factors secreted by parasite-infected cells in the bottom chamber. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS.
Control mock infected cardiac microvascular endothelial cell sample (24h; top)
Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell top, bathed with media from the mock-infected bottom HMVECs, harvested 24 hours post mock infection with 2% FBS medium. In the transwell setup, cells in the top chamber served as reporters for the effect of all difusible factors secreted by mock-infected cells in the bottom chamber. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS.

interferon-alpha-A/D study 1 (early) / untreated BE(2)-C cell sample (mature)

Relative Expression (log2-ratio):8.16859
Number of Samples:3 / 3
Experimental interferon-alpha-A/D study 1 (early)
Cultured BE(2)-C cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics and incubated with universal type I IFN (IFN-alpha-A/D) for 6 hours before samples were taken. ATC code:---
Control untreated BE(2)-C cell sample (mature)
Differentiated, cultured BE(2)-C neuroblastoma cells. Cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics before samples were taken.

dendritic cell study 7 (IL-4 mddc) / dendritic cell study 7 (IL-15 mddc)

Relative Expression (log2-ratio):-7.6635237
Number of Samples:3 / 3
Experimental dendritic cell study 7 (IL-4 mddc)
Mature IL-4 dendritic cells differentiated from CD14+ monocytes obtained from healthy donors. Monocytes were seeded in RPMI and supplemented with 2.5 % heat-inactivated human AB serum. Monocytes were cultured with 800 IU/mL granulocyte macrophage colony-stimulating factor and 20 ng/mL IL-4 in order to generate immature IL-4 DCs. Conventional maturation cocktail, comprising 10 ng/mL tumor necrosis factor-α, 10 ng/mL interleukin-1β, 15 ng/ml interleukin-6 and 1 µg/mL prostaglandin E2 was added for 48 hours. CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) by Ficoll density gradient centrifugation and purified by positive magnetic cell selection.
Control dendritic cell study 7 (IL-15 mddc)
Mature IL-15 dendritic cells differentiated from CD14+ monocytes obtained from healthy donors. Monocytes were seeded in RPMI and supplemented with 2.5 % heat-inactivated human AB serum. Differentiation was induced with 800 IU/mL granulocyte macrophage colony-stimulating factor and 200 ng/mL IL-15 to obtain immature IL-15 DCs. A TLR-activating maturation cocktail, comprising 3 µg/mL R848, 2.5 ng/mL tumor necrosis factor-α, 250 ng/mL interferon-γ and 1 µg/mL prostaglandin E2 was added after 24-48 hours of differentiation for 18-20 hours. CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) by Ficoll density gradient centrifugation and purified by positive magnetic cell selection.

human rhinovirus study 3 / control bronchial epithelial cell sample

Relative Expression (log2-ratio):7.444031
Number of Samples:4 / 4
Experimental human rhinovirus study 3
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to an infection with human rhinovirus 16 (HRV-16; MOI=1) for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the HRV-16 infection. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.
Control control bronchial epithelial cell sample
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and left untreated for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight and then for 24 hours. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.

IFN-a2b study 2 / untreated mouse-derived ovarian cancer cell (sidep.) sample

Relative Expression (log2-ratio):7.228531
Number of Samples:2 / 2
Experimental IFN-a2b study 2
Side population subset of ovarian cancer cells derived from the malignant effusion developed in mice injected with cells obtained from ascitic fluid of ovarian cancer bearing-patients. Cells were treated with 1000 IU/ml IFN-alpha2b for 5 hours.
Control untreated mouse-derived ovarian cancer cell (sidep.) sample
Side population subset of ovarian cancer cells derived from the malignant effusion developed in mice injected with cells obtained from ascitic fluid of ovarian cancer bearing-patients. Cells were untreated.

interferon-alpha-A/D study 2 (early) / untreated BE(2)-C cell sample (immature)

Relative Expression (log2-ratio):7.1129723
Number of Samples:3 / 3
Experimental interferon-alpha-A/D study 2 (early)
Undifferentiated, cultured BE(2)-C cells were incubated with universal type I IFN (IFNa-A/D) for 6 hours before samples were taken. ATC code:---
Control untreated BE(2)-C cell sample (immature)
Undifferentiated, cultured BE(2)-C neuroblastoma cell samples.

heat shock study 1 (LPS) / untreated THP-1 cell sample

Relative Expression (log2-ratio):7.0373
Number of Samples:3 / 3
Experimental heat shock study 1 (LPS)
Cultured THP-1 mononuclear cells were treated with heat shock (43°C; 1h) and lipopolysaccharide (1ug/ml; 4h).
Control untreated THP-1 cell sample
Cultured THP-1 mononuclear cells were cultured in basal growth media at 37°C.

smoking study 81 (RV16; CSE) / smoking study 81 (CSE)

Relative Expression (log2-ratio):7.015456
Number of Samples:4 / 4
Experimental smoking study 81 (RV16; CSE)
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to a treatment with 50% cigarette smoke extract (CSE) and an infection with human rhinovirus 16 (HRV-16; MOI=1) for 24 hours. In order to prepare CSE, one reference cigarette 3R4F was bubbled into 4 ml of hydrocortisone-free bronchial epithelial cell growth medium (BEGM) for 5 minutes using a syringe apparatus. Absorbance of the obtained CSE was adjusted to 0.15 at 320 nm and the resulting CSE was used at 50% concentration. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the CSE stimulation and HRV-16 infection. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.
Control smoking study 81 (CSE)
Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to a treatment with 50% cigarette smoke extract (CSE) for 24 hours. In order to prepare CSE, one reference cigarette 3R4F was bubbled into 4 ml of hydrocortisone-free bronchial epithelial cell growth medium (BEGM) for 5 minutes using a syringe apparatus. Absorbance of the obtained CSE was adjusted to 0.15 at 320 nm and the resulting CSE was used at 50% concentration. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the CSE stimulation. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis.