TOP TEN perturbations for 38584_at (Homo sapiens)
Organism: Homo sapiens
Gene: 38584_at
Selected probe(set): 229450_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 38584_at (229450_at) across 6674 perturbations tested by GENEVESTIGATOR:
smoking study 81 (CSE) / human rhinovirus study 3
Relative Expression (log2-ratio):-8.683232Number of Samples:4 / 4
| Experimental | smoking study 81 (CSE) |
| Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to a treatment with 50% cigarette smoke extract (CSE) for 24 hours. In order to prepare CSE, one reference cigarette 3R4F was bubbled into 4 ml of hydrocortisone-free bronchial epithelial cell growth medium (BEGM) for 5 minutes using a syringe apparatus. Absorbance of the obtained CSE was adjusted to 0.15 at 320 nm and the resulting CSE was used at 50% concentration. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the CSE stimulation. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis. | |
| Control | human rhinovirus study 3 |
| Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to an infection with human rhinovirus 16 (HRV-16; MOI=1) for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the HRV-16 infection. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis. |
T. cruzi study 1 (HMVEC; 24h; bottom) / mock infected cardiac microvascular endothelial cell sample (24h; bottom)
Relative Expression (log2-ratio):8.588274Number of Samples:2 / 2
| Experimental | T. cruzi study 1 (HMVEC; 24h; bottom) |
| Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell bottom, infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 24 hours post infection. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS. | |
| Control | mock infected cardiac microvascular endothelial cell sample (24h; bottom) |
| Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell bottom, mock-infected and harvested 24 hours post mock infection with 2% FBS medium. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS. |
T. cruzi study 1 (HMVEC; 24h; top) / mock infected cardiac microvascular endothelial cell sample (24h; top)
Relative Expression (log2-ratio):8.583575Number of Samples:2 / 2
| Experimental | T. cruzi study 1 (HMVEC; 24h; top) |
| Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell top, bathed with media from the HMVECs infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 24 hours post infection. In the transwell setup, cells in the top chamber served as reporters for the effect of all difusible factors secreted by parasite-infected cells in the bottom chamber. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS. | |
| Control | mock infected cardiac microvascular endothelial cell sample (24h; top) |
| Human cardiac microvascular endothelial cells (HMVECs) grown in the transwell top, bathed with media from the mock-infected bottom HMVECs, harvested 24 hours post mock infection with 2% FBS medium. In the transwell setup, cells in the top chamber served as reporters for the effect of all difusible factors secreted by mock-infected cells in the bottom chamber. Cells were maintained in endothelial cell basal medium-2 and endothelial cell growth supplements including 5% FBS. |
interferon-alpha-A/D study 1 (early) / untreated BE(2)-C cell sample (mature)
Relative Expression (log2-ratio):8.16859Number of Samples:3 / 3
| Experimental | interferon-alpha-A/D study 1 (early) |
| Cultured BE(2)-C cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics and incubated with universal type I IFN (IFN-alpha-A/D) for 6 hours before samples were taken. ATC code:--- | |
| Control | untreated BE(2)-C cell sample (mature) |
| Differentiated, cultured BE(2)-C neuroblastoma cells. Cells were differentiated with 10 uM all-trans retinoic acid (RA) for 3 weeks into cells with mature neuron cell characteristics before samples were taken. |
dendritic cell study 7 (IL-4 mddc) / dendritic cell study 7 (IL-15 mddc)
Relative Expression (log2-ratio):-7.6635237Number of Samples:3 / 3
| Experimental | dendritic cell study 7 (IL-4 mddc) |
| Mature IL-4 dendritic cells differentiated from CD14+ monocytes obtained from healthy donors. Monocytes were seeded in RPMI and supplemented with 2.5 % heat-inactivated human AB serum. Monocytes were cultured with 800 IU/mL granulocyte macrophage colony-stimulating factor and 20 ng/mL IL-4 in order to generate immature IL-4 DCs. Conventional maturation cocktail, comprising 10 ng/mL tumor necrosis factor-α, 10 ng/mL interleukin-1β, 15 ng/ml interleukin-6 and 1 µg/mL prostaglandin E2 was added for 48 hours. CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) by Ficoll density gradient centrifugation and purified by positive magnetic cell selection. | |
| Control | dendritic cell study 7 (IL-15 mddc) |
| Mature IL-15 dendritic cells differentiated from CD14+ monocytes obtained from healthy donors. Monocytes were seeded in RPMI and supplemented with 2.5 % heat-inactivated human AB serum. Differentiation was induced with 800 IU/mL granulocyte macrophage colony-stimulating factor and 200 ng/mL IL-15 to obtain immature IL-15 DCs. A TLR-activating maturation cocktail, comprising 3 µg/mL R848, 2.5 ng/mL tumor necrosis factor-α, 250 ng/mL interferon-γ and 1 µg/mL prostaglandin E2 was added after 24-48 hours of differentiation for 18-20 hours. CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) by Ficoll density gradient centrifugation and purified by positive magnetic cell selection. |
human rhinovirus study 3 / control bronchial epithelial cell sample
Relative Expression (log2-ratio):7.444031Number of Samples:4 / 4
| Experimental | human rhinovirus study 3 |
| Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to an infection with human rhinovirus 16 (HRV-16; MOI=1) for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the HRV-16 infection. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis. | |
| Control | control bronchial epithelial cell sample |
| Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and left untreated for 24 hours. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight and then for 24 hours. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis. |
IFN-a2b study 2 / untreated mouse-derived ovarian cancer cell (sidep.) sample
Relative Expression (log2-ratio):7.228531Number of Samples:2 / 2
| Experimental | IFN-a2b study 2 |
| Side population subset of ovarian cancer cells derived from the malignant effusion developed in mice injected with cells obtained from ascitic fluid of ovarian cancer bearing-patients. Cells were treated with 1000 IU/ml IFN-alpha2b for 5 hours. | |
| Control | untreated mouse-derived ovarian cancer cell (sidep.) sample |
| Side population subset of ovarian cancer cells derived from the malignant effusion developed in mice injected with cells obtained from ascitic fluid of ovarian cancer bearing-patients. Cells were untreated. |
interferon-alpha-A/D study 2 (early) / untreated BE(2)-C cell sample (immature)
Relative Expression (log2-ratio):7.1129723Number of Samples:3 / 3
| Experimental | interferon-alpha-A/D study 2 (early) |
| Undifferentiated, cultured BE(2)-C cells were incubated with universal type I IFN (IFNa-A/D) for 6 hours before samples were taken. ATC code:--- | |
| Control | untreated BE(2)-C cell sample (immature) |
| Undifferentiated, cultured BE(2)-C neuroblastoma cell samples. |
heat shock study 1 (LPS) / untreated THP-1 cell sample
Relative Expression (log2-ratio):7.0373Number of Samples:3 / 3
| Experimental | heat shock study 1 (LPS) |
| Cultured THP-1 mononuclear cells were treated with heat shock (43°C; 1h) and lipopolysaccharide (1ug/ml; 4h). | |
| Control | untreated THP-1 cell sample |
| Cultured THP-1 mononuclear cells were cultured in basal growth media at 37°C. |
smoking study 81 (RV16; CSE) / smoking study 81 (CSE)
Relative Expression (log2-ratio):7.015456Number of Samples:4 / 4
| Experimental | smoking study 81 (RV16; CSE) |
| Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to a treatment with 50% cigarette smoke extract (CSE) and an infection with human rhinovirus 16 (HRV-16; MOI=1) for 24 hours. In order to prepare CSE, one reference cigarette 3R4F was bubbled into 4 ml of hydrocortisone-free bronchial epithelial cell growth medium (BEGM) for 5 minutes using a syringe apparatus. Absorbance of the obtained CSE was adjusted to 0.15 at 320 nm and the resulting CSE was used at 50% concentration. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the CSE stimulation and HRV-16 infection. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis. | |
| Control | smoking study 81 (CSE) |
| Human bronchial epithelial (HBE) cells isolated from non-smoking individuals and subjected to a treatment with 50% cigarette smoke extract (CSE) for 24 hours. In order to prepare CSE, one reference cigarette 3R4F was bubbled into 4 ml of hydrocortisone-free bronchial epithelial cell growth medium (BEGM) for 5 minutes using a syringe apparatus. Absorbance of the obtained CSE was adjusted to 0.15 at 320 nm and the resulting CSE was used at 50% concentration. HBE cells were isolated by protease digestion of dissected airways and grown in BEGM. HBE cells were cultured in hydrocortisne-free BEGM overnight before the CSE stimulation. Cells were isolated from subjects that died from cerebrovascular causes or accidental head trauma, and had negative serologies for HIV1/2, HTLV1/2, hepatitis A, B and C, and syphilis. |