TOP TEN perturbations for 38631_at (Homo sapiens)

Organism: Homo sapiens
Gene: 38631_at
Selected probe(set): 202510_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 38631_at (202510_s_at) across 6674 perturbations tested by GENEVESTIGATOR:

TNF-ɑ; TGF-ß2 study 1 (early) / untreated ARPE-19 cell sample

Relative Expression (log2-ratio):6.13023
Number of Samples:5 / 3
Experimental TNF-ɑ; TGF-ß2 study 1 (early)
ARPE-19 retina pigment epithelial cell samples treated with TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml). Samples were taken 1 hour and 6 hours after treatment.
Control untreated ARPE-19 cell sample
Untreated ARPE-19 retina pigment epithelial cell samples harvested before adding TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml).

TNF-ɑ; TGF-ß2 study 1 (intermediate) / untreated ARPE-19 cell sample

Relative Expression (log2-ratio):4.9847918
Number of Samples:6 / 3
Experimental TNF-ɑ; TGF-ß2 study 1 (intermediate)
ARPE-19 retina pigment epithelial cell samples treated with TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml). Samples were taken 16 and 24 hours after treatment.
Control untreated ARPE-19 cell sample
Untreated ARPE-19 retina pigment epithelial cell samples harvested before adding TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml).

TNF-ɑ study 14 (100ng/ml) / vehicle (HBSS) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):4.3918104
Number of Samples:3 / 3
Experimental TNF-ɑ study 14 (100ng/ml)
Bronchial epithelial cells (NHBE) treated with 100 ng/ml TNFalpha for 4 hours. NHBE cells were derived from a 63 years old, male donor.
Control vehicle (HBSS) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (HBSS) for 4 hours. NHBE cells were derived from a 63 years old, male donor.

TNF-ɑ study 17 (4h) / osteoarthritis study 6

Relative Expression (log2-ratio):4.2254467
Number of Samples:3 / 6
Experimental TNF-ɑ study 17 (4h)
Synovial fibroblast samples isolated from affected knee of patients with osteoarthritis (OA) were stimulated by 10 ng/ml of TNF-ɑ in serum-free DMEM for 4 hours. Average duration of OA: 4.4 ± 0.6 years, all patients were rheumatoid factor negative. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 13.2 ± 2.9 mg/l. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads® M-450. Before the TNF-ɑ stimulation, fibroblasts were cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS.
Control osteoarthritis study 6
Synovial fibroblast samples isolated from affected knee of patients with osteoarthritis (OA). Average duration of OA: 4.4 ± 0.6 years, all patients were rheumatoid factor negative. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 13.2 ± 2.9 mg/l. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads® M-450. The fibroblasts were than cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS. Prior RNA isolation, the fibroblasts were washed in serum free DMEM.

TNF-ɑ study 16 (4h) / rheumatoid arthritis study 10

Relative Expression (log2-ratio):4.214776
Number of Samples:3 / 6
Experimental TNF-ɑ study 16 (4h)
Synovial fibroblast samples isolated from affected knee of patients with rheumatoid factor positive rheumatoid arthritis (RA) were stimulated by 10 ng/ml of TNF-ɑ in serum-free DMEM for 4 hours. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads® M-450. Before the TNF-ɑ stimulation, fibroblasts were cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS. The RA was classified according to the criteria of the American College of Rheumatology. Average duration of RA: 11.5 ± 0.5 years. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 38.1 ± 7.2 mg/l.
Control rheumatoid arthritis study 10
Synovial fibroblast samples isolated from affected knee of patients with rheumatoid factor positive rheumatoid arthritis (RA), classified according to the criteria of the American College of Rheumatology. Average duration of RA: 11.5 ± 0.5 years. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 38.1 ± 7.2 mg/l. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads® M-450. The fibroblasts were than cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS. Prior RNA isolation, the fibroblasts were washed in serum free DMEM.

TNF-ɑ study 8 (100ng/ml) / vehicle (HBSS) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):4.197981
Number of Samples:3 / 3
Experimental TNF-ɑ study 8 (100ng/ml)
Bronchial epithelial cells (NHBE) treated with 100 ng/ml TNFalpha for 4 hours. NHBE cells were derived from a male donor.
Control vehicle (HBSS) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (HBSS) for 4 hours. NHBE cells were derived from a male donor.

TNF-ɑ study 13 (100ng/ml) / vehicle (HBSS) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):4.195442
Number of Samples:3 / 3
Experimental TNF-ɑ study 13 (100ng/ml)
Bronchial epithelial cells (NHBE) treated with 100 ng/ml TNFalpha for 2 hours. NHBE cells were derived from a 63 years old, male donor.
Control vehicle (HBSS) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (HBSS) for 2 hours. NHBE cells were derived from a 63 years old, male donor.

TNF-ɑ study 14 (30ng/ml) / vehicle (HBSS) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):4.1912155
Number of Samples:3 / 3
Experimental TNF-ɑ study 14 (30ng/ml)
Bronchial epithelial cells (NHBE) treated with 30 ng/ml TNFalpha for 4 hours. NHBE cells were derived from a 63 years old, male donor.
Control vehicle (HBSS) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (HBSS) for 4 hours. NHBE cells were derived from a 63 years old, male donor.

LPS study 2 / untreated THP-1 cell sample

Relative Expression (log2-ratio):4.071679
Number of Samples:3 / 3
Experimental LPS study 2
Cultured THP-1 mononuclear cells were treated with lipopolysaccharide (1 microgram/ml) for 4 hours at 37°C. ATC code:---
Control untreated THP-1 cell sample
Cultured THP-1 mononuclear cells were cultured in basal growth media at 37°C.

TNF-ɑ; TGF-ß2 study 1 (late) / untreated ARPE-19 cell sample

Relative Expression (log2-ratio):4.054311
Number of Samples:6 / 3
Experimental TNF-ɑ; TGF-ß2 study 1 (late)
ARPE-19 retina pigment epithelial cell samples treated with TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml). Samples were taken 42 and 60 hours after treatment.
Control untreated ARPE-19 cell sample
Untreated ARPE-19 retina pigment epithelial cell samples harvested before adding TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml).