TOP TEN perturbations for 38754_at (Homo sapiens)
Organism: Homo sapiens
Gene: 38754_at
Selected probe(set): 209230_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 38754_at (209230_s_at) across 6674 perturbations tested by GENEVESTIGATOR:
SDF study 4 / untreated MDA Mb231 cell sample
Relative Expression (log2-ratio):5.3526564Number of Samples:3 / 6
| Experimental | SDF study 4 |
| MDA Mb231 cells treated with 75nM stromal derived factor (SDF; Cxcl12) for 6h. | |
| Control | untreated MDA Mb231 cell sample |
| Untreated MDA Mb231 cells harvested after 6h. |
connective/soft tissue cancer study 1 (PDX; connective and soft tissue, synovial sarcoma, spindle cell; primary) / connective/soft tissue cancer study 1 (PDX; connective and soft tissue, liposarcoma, NOS; primary)
Relative Expression (log2-ratio):-4.846262Number of Samples:6 / 2
| Experimental | connective/soft tissue cancer study 1 (PDX; connective and soft tissue, synovial sarcoma, spindle cell; primary) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary connective and soft tissue, synovial sarcoma, spindle cell of the soft tissue (subcutaneously implanted). | |
| Control | connective/soft tissue cancer study 1 (PDX; connective and soft tissue, liposarcoma, NOS; primary) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary connective and soft tissue, liposarcoma, NOS of the soft tissue (subcutaneously implanted). |
renal cell carcinoma study 6 (chromophobe type) / normal kidney tissue (fetal)
Relative Expression (log2-ratio):4.559311Number of Samples:4 / 2
| Experimental | renal cell carcinoma study 6 (chromophobe type) |
| Tumor tissue samples from the kidney of patients with chromophobe renal cell carcinoma (cRCC). | |
| Control | normal kidney tissue (fetal) |
| Normal fetal kidney tissue samples. |
pancreatic cancer study 3 (ipmn) / normal pancreas tissue
Relative Expression (log2-ratio):-4.5070305Number of Samples:3 / 6
| Experimental | pancreatic cancer study 3 (ipmn) |
| Intraductal papillary mucinous neoplasm from patients with pancreatic cancer. | |
| Control | normal pancreas tissue |
| Normal human pancreatic tissue. |
HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2) / HIF-1a/HIF-2a depletion study 1 (normoxia; AB81)
Relative Expression (log2-ratio):-4.4769354Number of Samples:3 / 3
| Experimental | HIF-1a/HIF-2a depletion study 1 (hypoxia; HK2) |
| Proximal tubule epithelial cell line HK-2 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (20% O2). HK-2 cells were cultured in DMEM/F-12 supplemented with 10% fetal calf serum (FCS), 1% ITS, hydrocortisone, and antibiotics. The cells were incubated at 37 °C. | |
| Control | HIF-1a/HIF-2a depletion study 1 (normoxia; AB81) |
| Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with normoxia (20% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 °C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 °C in 6-well plates. |
hepatocyte (ESC) / HepaRG
Relative Expression (log2-ratio):-4.3695498Number of Samples:8 / 12
| Experimental | hepatocyte (ESC) |
| Hepatocyte-like cells differentiated from embryonic stem cells (ESC) | |
| Control | HepaRG |
| Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code: |
pancreatic cancer study 3 (ipmc) / normal pancreas tissue
Relative Expression (log2-ratio):-4.132494Number of Samples:6 / 6
| Experimental | pancreatic cancer study 3 (ipmc) |
| Intraductal papillary mucinous carcinoma from patients with pancreatic cancer. | |
| Control | normal pancreas tissue |
| Normal human pancreatic tissue. |
FoxO3 H212R overexpr. study 1 / FoxO3 overexpr. study 1
Relative Expression (log2-ratio):-4.0333185Number of Samples:3 / 3
| Experimental | FoxO3 H212R overexpr. study 1 |
| Human umbilical vein endothelial cells (HUVECs) transfected with retroviral pBP FoxO3.A3.ER.H212R vector for 4OHT (4-hydroxy-(Z)-tamoxifen) inducible expression of mutated FoxO3 (forkhead box O3) H212R, treated with 4OHT for 12 hours. 72 hours postinfection, cells were selected for puromycin resistance by adding 2 g/ml puromycin overnight. Further, cells were reseeded in puromycin-free medium, treated for 12 hours with 4OHT, and harvested for total RNA isolation. Cells from passage 3 were used for infection and maximally passaged once for the experiments. ATC code:--- | |
| Control | FoxO3 overexpr. study 1 |
| Human umbilical vein endothelial cells (HUVECs) transfected with retroviral pBP-FoxO3.A3.ER vector for 4OHT (4-hydroxy-(Z)-tamoxifen) inducible expression of FoxO3 (forkhead box O3), treated with 4OHT for 12 hours. 72 hours postinfection, cells were selected for puromycin resistance by adding 2 g/ml puromycin overnight. Further, cells were reseeded in puromycin-free medium, treated for 12 hours with 4OHT, and harvested for total RNA isolation. Cells from passage 3 were used for infection and maximally passaged once for the experiments. ATC code:--- |
FoxO3 overexpr. study 1 / 4-hydroxytamoxifen study 1 (empty vector)
Relative Expression (log2-ratio):4.0198736Number of Samples:3 / 3
| Experimental | FoxO3 overexpr. study 1 |
| Human umbilical vein endothelial cells (HUVECs) transfected with retroviral pBP-FoxO3.A3.ER vector for 4OHT (4-hydroxy-(Z)-tamoxifen) inducible expression of FoxO3 (forkhead box O3), treated with 4OHT for 12 hours. 72 hours postinfection, cells were selected for puromycin resistance by adding 2 g/ml puromycin overnight. Further, cells were reseeded in puromycin-free medium, treated for 12 hours with 4OHT, and harvested for total RNA isolation. Cells from passage 3 were used for infection and maximally passaged once for the experiments. ATC code:--- | |
| Control | 4-hydroxytamoxifen study 1 (empty vector) |
| Human umbilical vein endothelial cells (HUVECs) transfected with empty retroviral pBabe puro vector designed for 4OHT (4-hydroxy-(Z)-tamoxifen) inducible expression, treated with 4OHT for 12 hours. 72 hours postinfection, cells were selected for puromycin resistance by adding 2 g/ml puromycin overnight. Further, cells were reseeded in puromycin-free medium, treated for 12 hours with 4OHT, and harvested for total RNA isolation. Cells from passage 3 were used for infection and maximally passaged once for the experiments. ATC code:--- |
EWS/FLI-1 depletion study 3 (FLI-1 siRNA) / EWS/FLI-1 depletion study 3 (NT siRNA)
Relative Expression (log2-ratio):3.8461123Number of Samples:4 / 2
| Experimental | EWS/FLI-1 depletion study 3 (FLI-1 siRNA) |
| Neuroepitheliomal SK-N-MC cell tranfected with 10nM FLI-1 siRNA to knock down the EWS/FLI-1 fusion within the transfected cell line. Another identical transfection was done 24 hours later and samples were taken 48 hours after the first transfection. | |
| Control | EWS/FLI-1 depletion study 3 (NT siRNA) |
| Neuroepitheliomal SK-N-MC cells transfected with 1 nM non-targeting siRNA (NT siRNA) for 24 hours. Another identical transfection was done 24 hours later and samples were taken 48 hours after the first transfection. |