TOP TEN perturbations for 38772_at (Homo sapiens)
Organism: Homo sapiens
Gene: 38772_at
Selected probe(set): 201289_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 38772_at (201289_at) across 6674 perturbations tested by GENEVESTIGATOR:
renal cell carcinoma study 6 (chromophobe type) / normal kidney tissue (adult)
Relative Expression (log2-ratio):-6.051467Number of Samples:4 / 3
| Experimental | renal cell carcinoma study 6 (chromophobe type) |
| Tumor tissue samples from the kidney of patients with chromophobe renal cell carcinoma (cRCC). | |
| Control | normal kidney tissue (adult) |
| Normal adult kidney tissue samples. |
colorectal cancer study 8 (adjacent) / normal colon mucosa tissue
Relative Expression (log2-ratio):5.9575863Number of Samples:12 / 10
| Experimental | colorectal cancer study 8 (adjacent) |
| Tissue samples of normal-appearing and non-tumorous colonic mucosa of Asiatic individuals with early-onset colorectal carcinoma after surgery. | |
| Control | normal colon mucosa tissue |
| Tissue samples normal colon mucosa of healthy Asiatic individuals collected by colonoscopy for reasons of abdominal pain, change in bowel habits, and bleeding due to hemorrhoids. |
A. fumigatus study 1 / uninfected immature dendritic cell sample
Relative Expression (log2-ratio):5.9554157Number of Samples:2 / 2
| Experimental | A. fumigatus study 1 |
| 5 x 106 immature dendritic cells were cultivated together with 5 x 106 A. fumigatus germ tubes for 6h until isolation of total RNA. | |
| Control | uninfected immature dendritic cell sample |
| 5 x 106 immature dendritic cells were cultivated without fungi. |
bladder cancer study 1 / normal bladder tissue
Relative Expression (log2-ratio):-5.7494097Number of Samples:3 / 3
| Experimental | bladder cancer study 1 |
| Low grade superficial tumor samples. | |
| Control | normal bladder tissue |
| Normal bladder tissue. |
insulin study 2 (adipocyte; TNF; 10ng/ml; 24h; insulin; 20nM; 2h) / untreated MSC derived adipocyte sample
Relative Expression (log2-ratio):5.4316883Number of Samples:2 / 2
| Experimental | insulin study 2 (adipocyte; TNF; 10ng/ml; 24h; insulin; 20nM; 2h) |
| Tumor necrosis factor (TNF) and insulin treated mature adipocytes were derived from human mesenchymal stem cells (MSC). MSC were obtained from the bone marrow withdrawn (posterior iliac crest of the pelvic bone) of normal, non-diabetic volunteers. On day 19 of differentiation, cells were treated with 10ng/ml TNF for 24hours to induce insulin resistance. Cells then underwent a 5 hours serum starvation followed by treatment with 20nM insulin for 2 hours. Cells were subsequently separated by centrifugation, adipocytes fraction (buoyant fraction, >90% mature adipocytes) was taken and subjected to RNA isolation. Expansion and differentiation of MSC: Cells at passage 2 were cultured in MSC growth media until passage 4 to 7 was reached. Fresh adipogenic differentiation media (ADM) containing 120 pM insulin, dexamethasone, IBMX, and indomethacin was added. The cultures were maintained in ADM for 14 days with media changes every 3–4 days. On day 14, the media was changed to adipogenic maintenance media (AMM) containing 120 pM insulin. On day 19 of differentiation, the adipocyte differentiation efficiency was 61-64 %, as determined by the number of cells that have intracellular lipid droplets, about 54% of the cells were positive for the adipocyte marker, peroxisome proliferator-activated receptor gamma (PPARc). All experiments were done using cells with passage number less than 8. insulin ATC code:,, , , | |
| Control | untreated MSC derived adipocyte sample |
| Untreated mature adipocytes were derived from human mesenchymal stem cells (MSC). MSC were obtained from the bone marrow withdrawn (posterior iliac crest of the pelvic bone) of normal, non-diabetic volunteers. On day 20 of differentiation, cells underwent a 5 hours serum starvation followed by 2 hours incubation. Cells were subsequently separated by centrifugation, adipocytes fraction (buoyant fraction, >90% mature adipocytes) was taken and subjected to RNA isolation. Expansion and differentiation of MSC: Cells at passage 2 were cultured in MSC growth media until passage 4 to 7 was reached. Fresh adipogenic differentiation media (ADM) containing 120 pM insulin, dexamethasone, IBMX, and indomethacin was added. The cultures were maintained in ADM for 14 days with media changes every 3–4 days. On day 14, the media was changed to adipogenic maintenance media (AMM) containing 120 pM insulin. On day 19 of differentiation, the adipocyte differentiation efficiency was 61-64 %, as determined by the number of cells that have intracellular lipid droplets, about 54% of the cells were positive for the adipocyte marker, peroxisome proliferator-activated receptor gamma (PPARc). All experiments were done using cells with passage number less than 8. |
HCC study 20 (CDX; Hep-G2; ectopic) / HCC study 20 (PDX; ectopic)
Relative Expression (log2-ratio):5.27462Number of Samples:3 / 11
| Experimental | HCC study 20 (CDX; Hep-G2; ectopic) |
| Tumor tissue biopsy samples from Hep-G2 cell line-derived xenograft (CDX) ectopically generated in SCID mice by injecting hepatocellular carcinoma cells subcutaneously. In order to establish ectopic models, cells were injected subcutaneously into the flank regions. The mice were euthanized after two weeks. | |
| Control | HCC study 20 (PDX; ectopic) |
| Tumor tissue biopsy samples from patient-derived xenograft (PDX) sample ectopically generated in SCID mice by injecting hepatocellular carcinoma cells subcutaneously. Donor tumor tissue was obtained intraoperatively during liver resection from three patients. All three patients had hepatocellular carcinoma confirmed by histology. In order to establish ectopic models, cells were injected subcutaneously into the flank regions. The mice were euthanized after two weeks. |
breast cancer study 24 / breast cancer study 22
Relative Expression (log2-ratio):5.090992Number of Samples:10 / 10
| Experimental | breast cancer study 24 |
| Freshly purified CD4+ tumor-infiltrating T cells derived from female primary invasive breast ductal carcinoma. For individual patient characteristics see detailed sample information. The initial tissue dissociation was performed mechanically, without enzymatic digestion. | |
| Control | breast cancer study 22 |
| Freshly purified CD4+ T cells from peripheral blood of female donors with primary invasive breast ductal carcinoma. For individual patient characteristics see detailed sample information. |
HCC study 20 (CDX; Hep-G2; orthotopic) / HCC study 20 (PDX; orthotopic)
Relative Expression (log2-ratio):4.9675407Number of Samples:5 / 11
| Experimental | HCC study 20 (CDX; Hep-G2; orthotopic) |
| Tumor tissue biopsy samples from Hep-G2 cell line-derived xenograft (CDX) orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks. | |
| Control | HCC study 20 (PDX; orthotopic) |
| Tumor tissue biopsy samples from patient-derived xenograft (PDX) sample orthotopically generated in SCID mice by injecting hepatocellular carcinoma cells directly into liver parenchyma. Donor tumor tissue was obtained intraoperatively during liver resection from three patients. All three patients had hepatocellular carcinoma confirmed by histology. In order to establish orthotopic model, the left lobe of liver was exposed through midline abdominal incision and cells were injected directly into liver parenchyma of mice. The mice were euthanized after two weeks. |
pancreatic cancer study 3 (ipmc) / normal pancreas tissue
Relative Expression (log2-ratio):-4.741666Number of Samples:6 / 6
| Experimental | pancreatic cancer study 3 (ipmc) |
| Intraductal papillary mucinous carcinoma from patients with pancreatic cancer. | |
| Control | normal pancreas tissue |
| Normal human pancreatic tissue. |
oxyphilic adenoma study 1 / normal kidney tissue (adult)
Relative Expression (log2-ratio):-4.7118616Number of Samples:4 / 3
| Experimental | oxyphilic adenoma study 1 |
| Tumor tissue samples from the kidney of patients with oxyphilic adenoma (renal oncocytoma). | |
| Control | normal kidney tissue (adult) |
| Normal adult kidney tissue samples. |