TOP TEN perturbations for 38804_at (Homo sapiens)
Organism: Homo sapiens
Gene: 38804_at
Selected probe(set): 201112_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 38804_at (201112_s_at) across 6674 perturbations tested by GENEVESTIGATOR:
zalypsis study 2 / untreated OPM1 cell sample
Relative Expression (log2-ratio):-2.9152489Number of Samples:2 / 2
| Experimental | zalypsis study 2 |
| OPM1 multiple myeloma cells treated in vitro with zalypsis (5 nM), a novel marine-derived compound with potent antimyeloma activity. Cells were harvested at the beginning of induction of cell death (15-20% cell death as assessed by Annexin V-FITC staining). ATC code:--- | |
| Control | untreated OPM1 cell sample |
| OPM1 multiple myeloma cells untreated. |
colorectal cancer study 4 (recurring) / adjacent colon tissue (recurring)
Relative Expression (log2-ratio):2.8874607Number of Samples:24 / 3
| Experimental | colorectal cancer study 4 (recurring) |
| LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer. Patients developed metastatic recurrence during follow-up after surgery. | |
| Control | adjacent colon tissue (recurring) |
| Histologically normal colon tissue samples from patients with primary colorectal cancer collected after resection. Tissue was further extracted using laser-capture microdissection (LCM). With metastatic recurrence after resection during follow-up. |
colorectal cancer study 4 (metastatic progression) / adjacent colon tissue (metastatic progression)
Relative Expression (log2-ratio):2.5584936Number of Samples:23 / 4
| Experimental | colorectal cancer study 4 (metastatic progression) |
| LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer with metastatic progression. | |
| Control | adjacent colon tissue (metastatic progression) |
| Histologically normal colon tissue samples from patients with primary colorectal cancer and metastatic progression collected after resection. Tissue was further extracted using laser-capture microdissection (LCM). |
colorectal cancer study 33 (carcinoma; colonic mucosa) / adjacent colon mucosa sample
Relative Expression (log2-ratio):2.4912357Number of Samples:5 / 5
| Experimental | colorectal cancer study 33 (carcinoma; colonic mucosa) |
| Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal carcinoma. | |
| Control | adjacent colon mucosa sample |
| Adjacent colon mucosa sample obtained by laser capture microdissection (LCM) from patients with colorectal cancer. |
colorectal cancer study 25 / adjacent colon tissue
Relative Expression (log2-ratio):2.3801584Number of Samples:94 / 17
| Experimental | colorectal cancer study 25 |
| Human primary colorectal carcinoma sample. | |
| Control | adjacent colon tissue |
| Normal colon tissue samples adjacent to tumor from patients with colorectal cancer. |
estrogen study 1 (late) / untreated MCF-7 cell sample
Relative Expression (log2-ratio):2.372961Number of Samples:3 / 3
| Experimental | estrogen study 1 (late) |
| MCF-7 cells were stimulated with 100 nM estrogen for 12 hours. ATC code:--- | |
| Control | untreated MCF-7 cell sample |
| MCF-7 cells untreated (0h). |
glioma study 16 (LN-18) / normal astrocyte sample
Relative Expression (log2-ratio):2.359603Number of Samples:2 / 3
| Experimental | glioma study 16 (LN-18) |
| Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2. | |
| Control | normal astrocyte sample |
| Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations. |
colorectal cancer study 4 (non-recurring) / adjacent colon tissue (non-recurring)
Relative Expression (log2-ratio):2.3385906Number of Samples:76 / 18
| Experimental | colorectal cancer study 4 (non-recurring) |
| LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer. Patients did not develop metastatic recurrence during follow-up after surgery. | |
| Control | adjacent colon tissue (non-recurring) |
| Histologically normal colon tissue samples from patients with primary colorectal cancer collected after resection. Tissue was further extracted using laser-capture microdissection (LCM). No metastatic recurrence during follow-up. |
EBNA2 overexpr. study 1 (24h) / control virus transfected EREB2-5 cell sample (24h)
Relative Expression (log2-ratio):2.3257065Number of Samples:3 / 3
| Experimental | EBNA2 overexpr. study 1 (24h) |
| EREB2-5 cell line stably transfected with the chimeric Epstein-Barr virus-encoded nuclear antigen 2 (EBNA2) and control expression plasmid coding for bacterial chloramphenicol acetyltransferase (CAT) gene and . CAT was used as a negative control. Briefly, before the CAT and EBNA2 induction, cells were maintained in RPMI media and deprived of estrogen for 3 days before doxycycline and estrogen was added. Expression of EBNA2 was induced after addition of estrogen and expression of CAT was induced by the addition of doxycycline to a final concentration of 100 ng/mL medium. Doxycycline induced cells were harvested 24 hours after induction. Because EBNA2 is essential for the proliferation of EBV-infected cells, EREB2-5 cells grow only in the presence of estrogen. Before the preparation of RNA, NGFR cells were purified by MACS separation to enrich the cells with a transcriptional response to doxycycline. | |
| Control | control virus transfected EREB2-5 cell sample (24h) |
| EREB2-5 cell line stably transfected with control expression plasmid coding for bacterial chloramphenicol acetyltransferase (CAT) gene. CAT was used as a negative control. Briefly, before the CAT induction, cells were maintained in RPMI media and deprived of estrogen for 3 days before doxycycline was added. Expression of CAT was induced by the addition of doxycycline to a final concentration of 100 ng/mL medium. Doxycycline induced cells were harvested 24 hours after induction. Estrogen withdrawal leads to the inactivation of EBNA2, resulting in cell cycle arrest. Before the preparation of RNA, NGFR cells were purified by MACS separation to enrich the cells with a transcriptional response to doxycycline. |
colorectal adenoma study 7 (colonic mucosa) / adjacent colon mucosa sample
Relative Expression (log2-ratio):2.2715054Number of Samples:5 / 5
| Experimental | colorectal adenoma study 7 (colonic mucosa) |
| Colonic mucosa samples obtained by laser capture microdissection (LCM) from patients with colorectal adenoma. | |
| Control | adjacent colon mucosa sample |
| Adjacent colon mucosa sample obtained by laser capture microdissection (LCM) from patients with colorectal cancer. |