TOP TEN perturbations for 38818_at (Homo sapiens)
Organism: Homo sapiens
Gene: 38818_at
Selected probe(set): 202277_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 38818_at (202277_at) across 6674 perturbations tested by GENEVESTIGATOR:
colorectal adenoma study 2 / normal colon tissue
Relative Expression (log2-ratio):-2.3770218Number of Samples:5 / 6
| Experimental | colorectal adenoma study 2 |
| Laser microdissected human adenoma sample. | |
| Control | normal colon tissue |
| Laser microdissected human colonic epithelial cells sample. |
SPTLC123 study 1 (siRNA) / control siRNA transfected HuH-7 cell sample
Relative Expression (log2-ratio):-2.2563848Number of Samples:3 / 3
| Experimental | SPTLC123 study 1 (siRNA) |
| Human HuH-7 hepatocarcinoma cell samples transfected with 20nM siRNA targeting SPTLC123 (serine transferase subunits 1, 2 and 3). Transfected cells were incubated for 24 hours at 37C, 5% CO2. The growth media (high-glucose, DMEM, 10% fetal bovine serum, 2 mM L-glutamine, 10 mM Hepes, and 1% penicillin-streptomycin) was replaced and cells were incubated for another 24 hours before the conditioned media (DMEM + 1% FBS + fatty acid free BSA) was added. Cells were incubated for another 24 hours prior to sampling. | |
| Control | control siRNA transfected HuH-7 cell sample |
| Human HuH-7 hepatocarcinoma cell samples transfected with 20nM scrambled, non-targeting siRNA. Transfected cells were incubated for 24 hours at 37C, 5% CO2. The growth media (high-glucose, DMEM, 10% fetal bovine serum, 2 mM L-glutamine, 10 mM Hepes, and 1% penicillin-streptomycin) was replaced and cells were incubated for another 24 hours before the conditioned media (DMEM + 1% FBS + fatty acid free BSA) was added. Cells were incubated for another 24 hours prior to sampling. |
oncolytic herpes simplex virus study 2 / mock infected peripheral nerve sheath tumor (S462) cell sample
Relative Expression (log2-ratio):-2.2248068Number of Samples:3 / 3
| Experimental | oncolytic herpes simplex virus study 2 |
| Human malignant peripheral nerve sheath tumor (S462) cells infected with G207, an ICP34.5-deleted oncolytic herpes simplex virus (oHSV) for 6 hours. | |
| Control | mock infected peripheral nerve sheath tumor (S462) cell sample |
| Human malignant peripheral nerve sheath tumor (S462) cells mock infected for 6 hours. |
DEGS1 study 1 (siRNA) / SPTLC123 study 1 (siRNA)
Relative Expression (log2-ratio):2.202773Number of Samples:3 / 3
| Experimental | DEGS1 study 1 (siRNA) |
| Human HuH-7 hepatocarcinoma cell samples transfected with 20nM siRNA targeting DEGS1 (dihydroceramide desaturase). Transfected cells were incubated for 24 hours at 37C, 5% CO2. The growth media (high-glucose, DMEM, 10% fetal bovine serum, 2 mM L-glutamine, 10 mM Hepes, and 1% penicillin-streptomycin) was replaced and cells were incubated for another 24 hours before the conditioned media (DMEM + 1% FBS + fatty acid free BSA) was added. Cells were incubated for another 24 hours prior to sampling. | |
| Control | SPTLC123 study 1 (siRNA) |
| Human HuH-7 hepatocarcinoma cell samples transfected with 20nM siRNA targeting SPTLC123 (serine transferase subunits 1, 2 and 3). Transfected cells were incubated for 24 hours at 37C, 5% CO2. The growth media (high-glucose, DMEM, 10% fetal bovine serum, 2 mM L-glutamine, 10 mM Hepes, and 1% penicillin-streptomycin) was replaced and cells were incubated for another 24 hours before the conditioned media (DMEM + 1% FBS + fatty acid free BSA) was added. Cells were incubated for another 24 hours prior to sampling. |
brefeldin A study 1 (0.5ug/ml; p53HCT116) / untreated p53HCT116 cell sample
Relative Expression (log2-ratio):2.1720896Number of Samples:2 / 3
| Experimental | brefeldin A study 1 (0.5ug/ml; p53HCT116) |
| Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:--- | |
| Control | untreated p53HCT116 cell sample |
| Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS. |
kidney transplantation study 11 (12 week) / normal blood sample
Relative Expression (log2-ratio):1.9390993Number of Samples:10 / 5
| Experimental | kidney transplantation study 11 (12 week) |
| Whole blood samples derived from kidney transplant patients 12 weeks post-transplantation. Samples were collected 12 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH). | |
| Control | normal blood sample |
| Blood samples derived from healthy control subjects. |
vMyb overexpr. study 1 (adenovirus) / control virus infected monocyte sample
Relative Expression (log2-ratio):-1.8993931Number of Samples:2 / 2
| Experimental | vMyb overexpr. study 1 (adenovirus) |
| Approximately 1x10^6 monocytes were infected with adenovirus expressing v-Myb and GFP for 16 hours, then total RNA was isolated. | |
| Control | control virus infected monocyte sample |
| Approximately 1x10^6 monocytes were infected with control adenovirus expressing GFP for 16 hours, then total RNA was isolated. |
myotonic dystrophy study 4 (deltoid muscle; DM-like) / normal skeletal muscle tisuue (fetal)
Relative Expression (log2-ratio):-1.8777161Number of Samples:3 / 3
| Experimental | myotonic dystrophy study 4 (deltoid muscle; DM-like) |
| Deltoid muscle biopsies obtained from patients with myotonic-like dystrophy (DMx; DM-like; no DMPK or ZNF9 expansion). | |
| Control | normal skeletal muscle tisuue (fetal) |
| Commercially purchased normal fetal skeletal muscle tissue samples. |
endometriosis study 6 (minimal/mild endo.; pro. MCP) / normal endometrium tissue (pro. MCP)
Relative Expression (log2-ratio):-1.8613176Number of Samples:12 / 20
| Experimental | endometriosis study 6 (minimal/mild endo.; pro. MCP) |
| Endometrial tissue samples from women with minimal or mild endometriosis and pelvic pain and/or infertility collected in the proliferative menstrual cycle phase (MCP). Endometriosis was diagnosed based on the revised American Fertility Society classification system. The MCP was determined by endometrial histology, confirmed by estradiol and progesterone serum levels and corroborated by 2 independent bioinformatics methods: clustering in unsupervised whole-transcriptome principal component analysis and cycle phase assignment classifier analysis. Patients with hormonal treatment within previous 3 months and presence of malignancy or major systemic disease were excluded. | |
| Control | normal endometrium tissue (pro. MCP) |
| Normal endometrial tissue samples from women collected in the proliferative menstrual cycle phase (MCP). The MCP was determined by endometrial histology, confirmed by estradiol and progesterone serum levels and corroborated by 2 independent bioinformatics methods: clustering in unsupervised whole-transcriptome principal component analysis and cycle phase assignment classifier analysis. |
brefeldin A study 1 (0.5ug/ml; HCT 116) / untreated HCT 116 cell sample
Relative Expression (log2-ratio):1.815402Number of Samples:3 / 3
| Experimental | brefeldin A study 1 (0.5ug/ml; HCT 116) |
| Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:--- | |
| Control | untreated HCT 116 cell sample |
| Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS. |