TOP TEN perturbations for 38879_at (Homo sapiens)

Organism: Homo sapiens
Gene: 38879_at
Selected probe(set): 205863_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 38879_at (205863_at) across 6674 perturbations tested by GENEVESTIGATOR:

IL-4; GM-CSF study 1 (late) / untreated monocyte sample

Relative Expression (log2-ratio):-7.961626
Number of Samples:6 / 12
Experimental IL-4; GM-CSF study 1 (late)
Monocytes, cultured with vehicle (DMSO/ethanol) and 500 U/ml IL-4 and 800 U/ml GM-CSF for 5 days. Cytokine treatment was repeated at day 3.
Control untreated monocyte sample
Freshly isolated human monocytes from healthy donors.

pediatric meningococcal sepsis study 1 (24h; lymphocyte) / normal lymphocyte (CD14-) sample

Relative Expression (log2-ratio):7.6161394
Number of Samples:5 / 4
Experimental pediatric meningococcal sepsis study 1 (24h; lymphocyte)
Lymphocyte (CD14-) samples obtained from blood samples of children with (suspected) meningococcal sepsis collected 24 hours after admission to the pediatric intensive care unit (PICU). Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells (monocytes) and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.
Control normal lymphocyte (CD14-) sample
Normal lymphocyte (CD14-) samples obtained from peripheral blood of healthy children admitted for elective minor non-infectious surgery or MRI. Ten ml of whole blood samples were used to perform a Ficoll density separation using Lymphoprep. Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.

pediatric meningococcal sepsis study 1 (8h; lymphocyte) / normal lymphocyte (CD14-) sample

Relative Expression (log2-ratio):7.1944294
Number of Samples:2 / 4
Experimental pediatric meningococcal sepsis study 1 (8h; lymphocyte)
Lymphocyte (CD14-) samples obtained from blood samples of children with (suspected) meningococcal sepsis collected 8 hours after admission to the pediatric intensive care unit (PICU). Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells (monocytes) and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.
Control normal lymphocyte (CD14-) sample
Normal lymphocyte (CD14-) samples obtained from peripheral blood of healthy children admitted for elective minor non-infectious surgery or MRI. Ten ml of whole blood samples were used to perform a Ficoll density separation using Lymphoprep. Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.

thermal injury study 1 (0-3d) / normal skin tissue

Relative Expression (log2-ratio):6.8433313
Number of Samples:3 / 3
Experimental thermal injury study 1 (0-3d)
Burn wound margin samples of the skin 0-3 days post thermal injury.
Control normal skin tissue
Normal skin tissue sample.

psoriasis study 5 (NCT00867100; lesional; baseline) / psoriasis study 5 (NCT00867100; non-lesional; baseline)

Relative Expression (log2-ratio):6.7394147
Number of Samples:24 / 24
Experimental psoriasis study 5 (NCT00867100; lesional; baseline)
Lesional skin punch biopsies derived from patients (Set 2 of clinical trial NCT00867100; aged 19-55) with moderate to severe plaque psoriasis (over more than 10% of body surface area and a PASI score greater 10). A 6mm punch biopsy was obtained from lesional skin at baseline.
Control psoriasis study 5 (NCT00867100; non-lesional; baseline)
Non-lesional skin punch biopsies derived from patients (Set 2 of clinical trial NCT00867100; aged 19-55) with moderate to severe plaque psoriasis (over more than 10% of body surface area and a PASI score greater 10). A 6mm punch biopsy was obtained from uninvolved, non-lesional skin at baseline.

pediatric meningococcal sepsis study 1 (72h; lymphocyte) / normal lymphocyte (CD14-) sample

Relative Expression (log2-ratio):6.736779
Number of Samples:2 / 4
Experimental pediatric meningococcal sepsis study 1 (72h; lymphocyte)
Lymphocyte (CD14-) samples obtained from blood samples of children with (suspected) meningococcal sepsis collected 72 hours after admission to the pediatric intensive care unit (PICU). Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells (monocytes) and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.
Control normal lymphocyte (CD14-) sample
Normal lymphocyte (CD14-) samples obtained from peripheral blood of healthy children admitted for elective minor non-infectious surgery or MRI. Ten ml of whole blood samples were used to perform a Ficoll density separation using Lymphoprep. Granulocytes and peripheral blood mononuclear cells (PBMCs) were isolated. From the PBMCs, CD14+ cells and CD14- cells (enriched for B- and T-cells) were separated using autoMACS sorting.

psoriasis study 20 (lesional) / psoriasis study 20 (non-lesional)

Relative Expression (log2-ratio):6.659308
Number of Samples:4 / 4
Experimental psoriasis study 20 (lesional)
Lesional skin punch biopsies derived from patients with chronic plaque psoriasis. 6 mm punch biopsies were obtained under local anesthesia preferentially near the central region of active plaques, except in cases where the center was poorly defined due to an irregular boundary. Regions near the edge of individual plaques were avoided to ensure that uninvolved skin was not included. Patients with one or more psoriasis plaques not limited to the scalp area were enrolled to the study. In case that only one plaque was present, a patient was admitted when that plaque occupied more than 1% of the body surface area. Patients were advised not to apply topical treatments for at least 1 week prior to biopsies and not to use systemic medications for at least 2 weeks prior to sample biopsies. Biopsies were snap-frozen in liquid nitrogen, stored at -80°C.
Control psoriasis study 20 (non-lesional)
Non-lesional skin punch biopsies derived from patients with chronic plaque psoriasis. 6 mm punch biopsies were obtained under local anesthesia from the buttocks and at least 10 cm away from the nearest active psoriasis plaque. Patients with one or more psoriasis plaques not limited to the scalp area were enrolled to the study. In case that only one plaque was present, a patient was admitted when that plaque occupied more than 1% of the body surface area. Patients were advised not to apply topical treatments for at least 1 week prior to biopsies and not to use systemic medications for at least 2 weeks prior to sample biopsies. Biopsies were snap-frozen in liquid nitrogen, stored at -80°C.

precursor-B-ALL study 7 (PDX; short-term; <10wk) / precursor-B-ALL study 7 (early relapse; <24m)

Relative Expression (log2-ratio):-6.5689526
Number of Samples:5 / 22
Experimental precursor-B-ALL study 7 (PDX; short-term; <10wk)
Leukemia cell samples isolated from spleen of patient derived xenografts (PDX) of precursor B-cell acute lymphoblastic leukemia (B-ALL) generated in NOD/SCID mice with time to manifestation of leukemia (TTL) less than 10 weeks (short-term). Cell suspensions containing more than 90% leukemia cells as estimated by flow cytometry were prepared from infiltrated spleens of leukemia bearing mice. Briefly, unconditioned NOD/SCID (NOD.CB17-Prkdcscid/NCrCrl) mice with a median age of 10 weeks were transplanted by injection of patient leukemia cells, which were isolated from bone marrow or peripheral blood of pediatric patients with BCP-ALL, into the lateral tail vein. Upon clear evidence for leukemia related morbidity, mice were killed and autopsy was performed. Leukemia was confirmed detecting leukemia cells in bone marrow, spleen and peripheral blood. Time to leukemia (TTL) was determined as weeks from transplant to clinical leukemia manifestation. Donor characteristics: 3 females and 9 males; 1-9 years old; good response to prednison; no fusion gene,;remision at day 33; non-high risk group; early relapse group (relapse within 24 months from diagnosis).
Control precursor-B-ALL study 7 (early relapse; <24m)
Leukemia cell samples isolated from bone marrow of pediatric patients with precursor B-cell acute lymphoblastic leukemia (B-ALL) with relapse within 24 months after diagnosis (early relapse). White blood cells were isolated through Ficoll-Hypaque density gradient centrifugation. All diagnostic leukemia samples were obtained before treatment from pediatric de novo B cell precursor ALL patients (BCP-ALL). Samples obtained from studies registred under NCT00430118 and NCT00613457.

thermal injury study 1 (>7d) / normal skin tissue

Relative Expression (log2-ratio):6.551424
Number of Samples:3 / 3
Experimental thermal injury study 1 (>7d)
Burn wound margin samples of the skin >7 days post thermal injury.
Control normal skin tissue
Normal skin tissue sample.

skin squamous cell carcinoma study 6 / normal skin tissue

Relative Expression (log2-ratio):6.53913
Number of Samples:4 / 64
Experimental skin squamous cell carcinoma study 6
Primary tumor tissue from the skin of patients with squamous cell carcinoma (SCC).
Control normal skin tissue
Normal skin samples from healthy donors.