TOP TEN perturbations for 38989_at (Homo sapiens)
Organism: Homo sapiens
Gene: 38989_at
Selected probe(set): 201678_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 38989_at (201678_s_at) across 6674 perturbations tested by GENEVESTIGATOR:
immune cell study 6 (PB; memory) / immune cell study 6 (PB; CD21lo)
Relative Expression (log2-ratio):-3.0078669Number of Samples:2 / 2
| Experimental | immune cell study 6 (PB; memory) |
| Memory B-cells (CD20+/CD10-/CD27+) isolated from peripheral blood (PB). B-cells were isolated by negative selection, labeled with mAb specific for CD20, CD10, CD21, and CD27 and sorted for CD20+/CD10-/CD27+. | |
| Control | immune cell study 6 (PB; CD21lo) |
| Immature (transitional) B-cells (CD20+/CD10+/CD27-/CD21lo) isolated from peripheral blood (PB) B-cells were isolated by negative selection, labeled with mAb specific for CD20, CD10, CD21, and CD27 and sorted for CD20+/CD10+/CD27-/CD21lo. |
immune cell study 10 (IgM only memory B-cell) / immune cell study 10 (naive B-cell)
Relative Expression (log2-ratio):-2.6140947Number of Samples:5 / 5
| Experimental | immune cell study 10 (IgM only memory B-cell) |
| IgM only memory B-cells (IgM+IgD-/lowCD27+) isolated from peripheral blood of healthy adult donors (between 25 and 60 years of age). Mononuclear cells were isolated by Ficoll–Paque density centrifugation and CD19+ B-cells were enriched by magnetic cell separation using the MACS system or by negative selection using the EasySep Human B-Cell Enrichment Kit. B-cell suspensions were stained with anti-CD27 APC, anti-IgD PE–Cy7, anti-IgM FITC, and anti-IgG PE or anti-CD21 PE antibodies and sorted with a FACSDiva cell sorter as IgM only memory B-cells. | |
| Control | immune cell study 10 (naive B-cell) |
| Naive B-cells (IgM+IgD+CD27-) isolated from peripheral blood of healthy adult donors (between 25 and 60 years of age). Mononuclear cells were isolated by Ficoll–Paque density centrifugation and CD19+ B-cells were enriched by magnetic cell separation using the MACS system or by negative selection using the EasySep Human B-Cell Enrichment Kit. B-cell suspensions were stained with anti-CD27 APC, anti-IgD PE–Cy7, anti-IgM FITC, and anti-IgG PE or anti-CD21 PE antibodies and sorted with a FACSDiva cell sorter as naive B cells. |
DLBCL study 7 (plasmablast-like) / DLBCL study 7 (centroblast-like)
Relative Expression (log2-ratio):-2.5911064Number of Samples:2 / 2
| Experimental | DLBCL study 7 (plasmablast-like) |
| Primary tumor samples from patients with unclassified diffuse large B-cell lymphoma. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS) and assigned to plasmablast-like group. Patients were treated according to standard protocols, and comparable doses of RCHOP- like regimens were used. No patient was treated with stem cell transplantation. | |
| Control | DLBCL study 7 (centroblast-like) |
| Primary tumor samples from patients with unclassified diffuse large B-cell lymphoma. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS) and assigned to centroblast-like group. Patients were treated according to standard protocols, and comparable doses of RCHOP- like regimens were used. No patient was treated with stem cell transplantation. |
immune cell study 10 (IgG memory B-cell) / immune cell study 10 (naive B-cell)
Relative Expression (log2-ratio):-2.3983812Number of Samples:5 / 5
| Experimental | immune cell study 10 (IgG memory B-cell) |
| Class switched IgG memory B-cells (IgG+CD27+) isolated from peripheral blood of healthy adult donors (between 25 and 60 years of age). Mononuclear cells were isolated by Ficoll–Paque density centrifugation and CD19+ B-cells were enriched by magnetic cell separation using the MACS system or by negative selection using the EasySep Human B-Cell Enrichment Kit. B-cell suspensions were stained with anti-CD27 APC, anti-IgD PE–Cy7, anti-IgM FITC, and anti-IgG PE or anti-CD21 PE antibodies and sorted with a FACSDiva cell sorter as class switched IgG memory B-cells. | |
| Control | immune cell study 10 (naive B-cell) |
| Naive B-cells (IgM+IgD+CD27-) isolated from peripheral blood of healthy adult donors (between 25 and 60 years of age). Mononuclear cells were isolated by Ficoll–Paque density centrifugation and CD19+ B-cells were enriched by magnetic cell separation using the MACS system or by negative selection using the EasySep Human B-Cell Enrichment Kit. B-cell suspensions were stained with anti-CD27 APC, anti-IgD PE–Cy7, anti-IgM FITC, and anti-IgG PE or anti-CD21 PE antibodies and sorted with a FACSDiva cell sorter as naive B cells. |
immune cell study 6 (PB; memory) / immune cell study 6 (PB; CD21hi)
Relative Expression (log2-ratio):-2.2852697Number of Samples:2 / 2
| Experimental | immune cell study 6 (PB; memory) |
| Memory B-cells (CD20+/CD10-/CD27+) isolated from peripheral blood (PB). B-cells were isolated by negative selection, labeled with mAb specific for CD20, CD10, CD21, and CD27 and sorted for CD20+/CD10-/CD27+. | |
| Control | immune cell study 6 (PB; CD21hi) |
| Immature (transitional) B-cells (CD20+/CD10+/CD27-/CD21hi) isolated from peripheral blood (PB). B-cells were isolated by negative selection, labeled with mAb specific for CD20, CD10, CD21, and CD27 and sorted for CD20+/CD10+/CD27-/CD21hi. |
immune cell study 10 (IgM memory B-cell) / immune cell study 10 (naive B-cell)
Relative Expression (log2-ratio):-2.153594Number of Samples:5 / 5
| Experimental | immune cell study 10 (IgM memory B-cell) |
| IgM memory B-cells (IgM+IgD+CD27+) isolated from peripheral blood of healthy adult donors (between 25 and 60 years of age). Mononuclear cells were isolated by Ficoll–Paque density centrifugation and CD19+ B-cells were enriched by magnetic cell separation using the MACS system or by negative selection using the EasySep Human B-Cell Enrichment Kit. B-cell suspensions were stained with anti-CD27 APC, anti-IgD PE–Cy7, anti-IgM FITC, and anti-IgG PE or anti-CD21 PE antibodies and sorted with a FACSDiva cell sorter as IgM memory B-cells. | |
| Control | immune cell study 10 (naive B-cell) |
| Naive B-cells (IgM+IgD+CD27-) isolated from peripheral blood of healthy adult donors (between 25 and 60 years of age). Mononuclear cells were isolated by Ficoll–Paque density centrifugation and CD19+ B-cells were enriched by magnetic cell separation using the MACS system or by negative selection using the EasySep Human B-Cell Enrichment Kit. B-cell suspensions were stained with anti-CD27 APC, anti-IgD PE–Cy7, anti-IgM FITC, and anti-IgG PE or anti-CD21 PE antibodies and sorted with a FACSDiva cell sorter as naive B cells. |
lactic acid study 1 (hypoxia) / untreated breast epithelial cell sample
Relative Expression (log2-ratio):2.0976868Number of Samples:3 / 3
| Experimental | lactic acid study 1 (hypoxia) |
| Breast epithelial cells exposed to 25mM lactic acid of pH6.7 and 2% hypoxia. ATC code: | |
| Control | untreated breast epithelial cell sample |
| Breast epithelial cells in control media under normoxia. |
DLBCL study 1 (germinal center B-cell like; unclassified) / DLBCL study 1 (activated B-cell like; unclassified)
Relative Expression (log2-ratio):2.0718699Number of Samples:2 / 11
| Experimental | DLBCL study 1 (germinal center B-cell like; unclassified) |
| Unclassified B-cells sorted from primary tumor samples from patients with malignant diffuse large B-cell lymphoma (germinal center B-cell like type). To isolate different B-cell subsets, tonsil tissues from DLBCL patients were sorted by fluorescence-activated cell sorting. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS). | |
| Control | DLBCL study 1 (activated B-cell like; unclassified) |
| Unclassified B-cells sorted from primary tumor samples from patients with malignant diffuse large B-cell lymphoma (activated B-cell like type). To isolate different B-cell subsets, tonsil tissues from DLBCL patients were sorted by fluorescence-activated cell sorting. Samples were also classified based on subset-specific B-cell-associated gene signature (BAGS). |
B-cell activation study 1 / unstimulated B-cell sample
Relative Expression (log2-ratio):-1.9880981Number of Samples:4 / 4
| Experimental | B-cell activation study 1 |
| Tonsillar B-cell samples stimulated with 10 µg/mL anti-CD40 antibody and 100 U/mL recombinant human IL-4. Cells were cultured under TH2-skewing conditions for 24 hours. | |
| Control | unstimulated B-cell sample |
| Unstimulated tonsillar B-cells cultured for 24 hours. |
precursor-B-ALL study 1 (t(12;21)(p12,q22)) / normal bone marrow sample
Relative Expression (log2-ratio):1.9194307Number of Samples:58 / 74
| Experimental | precursor-B-ALL study 1 (t(12;21)(p12,q22)) |
| Bone marrow samples of patients with precursor B-ALL (t(12;21)(p12,q22)/TEL-AML1). | |
| Control | normal bone marrow sample |
| Non-leukemic and healthy bone marrow sample. |