TOP TEN perturbations for 39098_at (Homo sapiens)
Organism: Homo sapiens
Gene: 39098_at
Selected probe(set): 212670_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 39098_at (212670_at) across 6674 perturbations tested by GENEVESTIGATOR:
retina pigment epithelium study 1 (adult) / retina pigment epithelium study 1 (fetal)
Relative Expression (log2-ratio):-3.8801727Number of Samples:2 / 6
| Experimental | retina pigment epithelium study 1 (adult) |
| Postmortem human native retina pigment epithelium samples from adult individuals. | |
| Control | retina pigment epithelium study 1 (fetal) |
| Human native fetal retina pigment epithelium samples obtained at gestation age between week 16-18. |
brain tumor study 1 (ependymoma) / normal brain tissue
Relative Expression (log2-ratio):3.8132505Number of Samples:46 / 12
| Experimental | brain tumor study 1 (ependymoma) |
| Primary tumor tissue sample from the brain of patients with ependymoma. | |
| Control | normal brain tissue |
| Histologically normal and non-neoplastic tissue sample from different brain anatomical sites of patients with primary brain tumors. |
TGF-ß study 7 (12h) / rheumatoid arthritis study 10
Relative Expression (log2-ratio):3.747569Number of Samples:3 / 6
| Experimental | TGF-ß study 7 (12h) |
| Synovial fibroblast samples isolated from affected knee of patients with rheumatoid factor positive rheumatoid arthritis (RA) were stimulated by 10 ng/ml of TGF-ß in serum-free DMEM for 12 hours. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads® M-450. Before the TGF-ß stimulation, fibroblasts were cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS. The RA was classified according to the criteria of the American College of Rheumatology. Average duration of RA: 11.5 ± 0.5 years. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 38.1 ± 7.2 mg/l. | |
| Control | rheumatoid arthritis study 10 |
| Synovial fibroblast samples isolated from affected knee of patients with rheumatoid factor positive rheumatoid arthritis (RA), classified according to the criteria of the American College of Rheumatology. Average duration of RA: 11.5 ± 0.5 years. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 38.1 ± 7.2 mg/l. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads® M-450. The fibroblasts were than cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS. Prior RNA isolation, the fibroblasts were washed in serum free DMEM. |
ovarian tumor study 28 (mucocarcinoid tumor) / ovarian tumor study 28 (carcinosarcoma)
Relative Expression (log2-ratio):-3.7303686Number of Samples:7 / 2
| Experimental | ovarian tumor study 28 (mucocarcinoid tumor) |
| Primary tumor tissue sample obtained from the ovary of female patients with malignant mucocarcinoid tumor. | |
| Control | ovarian tumor study 28 (carcinosarcoma) |
| Primary tumor tissue sample obtained from the ovary of female patients with carcinosarcoma. |
connective/soft tissue cancer study 1 (PDX; connective and soft tissue, sarcoma, NOS; metastatic) / connective/soft tissue cancer study 1 (PDX; connective and soft tissue, sarcoma, NOS; primary)
Relative Expression (log2-ratio):3.6817293Number of Samples:3 / 7
| Experimental | connective/soft tissue cancer study 1 (PDX; connective and soft tissue, sarcoma, NOS; metastatic) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from a metastasis of patients with primary connective and soft tissue, sarcoma, NOS of the soft tissue (subcutaneously implanted). Metastatic site of patient tumor sample is not reported. | |
| Control | connective/soft tissue cancer study 1 (PDX; connective and soft tissue, sarcoma, NOS; primary) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from patients with primary connective and soft tissue, sarcoma, NOS of the soft tissue (subcutaneously implanted). |
ovarian tumor study 28 (carcinosarcoma) / ovarian tumor study 28 (adenocarcinoma)
Relative Expression (log2-ratio):3.5422773Number of Samples:2 / 3
| Experimental | ovarian tumor study 28 (carcinosarcoma) |
| Primary tumor tissue sample obtained from the ovary of female patients with carcinosarcoma. | |
| Control | ovarian tumor study 28 (adenocarcinoma) |
| Primary tumor tissue sample obtained from the ovary of female patients with adenocarcinoma. |
TGF-ß study 8 (4h) / osteoarthritis study 6
Relative Expression (log2-ratio):3.5047092Number of Samples:3 / 6
| Experimental | TGF-ß study 8 (4h) |
| Synovial fibroblast samples isolated from affected knee of patients with osteoarthritis (OA) were stimulated by 10 ng/ml of TGF-ß in serum-free DMEM for 4 hours. Average duration of OA: 4.4 ± 0.6 years, all patients were rheumatoid factor negative. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 13.2 ± 2.9 mg/l. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads® M-450. Before the TGF-ß stimulation, fibroblasts were cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS. | |
| Control | osteoarthritis study 6 |
| Synovial fibroblast samples isolated from affected knee of patients with osteoarthritis (OA). Average duration of OA: 4.4 ± 0.6 years, all patients were rheumatoid factor negative. Patients were receiving non-steroidal antiinflammatory drugs. CRP: 13.2 ± 2.9 mg/l. Synovial fibroblasts were isolated from surgically obtained synovial membrane by trypsin/collagenase digestion, followed by negative purification using CD-14 Dynabeads® M-450. The fibroblasts were than cultured for four passages in DMEM medium supplemented with antibiotics and 10% FCS. Prior RNA isolation, the fibroblasts were washed in serum free DMEM. |
ovarian tumor study 28 (endometrioid carcinoma) / ovarian tumor study 28 (carcinosarcoma)
Relative Expression (log2-ratio):-3.4103947Number of Samples:6 / 2
| Experimental | ovarian tumor study 28 (endometrioid carcinoma) |
| Primary tumor tissue sample obtained from the ovary of female patients with endometrioid carcinoma. | |
| Control | ovarian tumor study 28 (carcinosarcoma) |
| Primary tumor tissue sample obtained from the ovary of female patients with carcinosarcoma. |
connective/soft tissue cancer study 1 (PDX; connective and soft tissue, leiomyosarcoma, NOS; metastatic) / connective/soft tissue cancer study 1 (PDX; connective and soft tissue, clear cell sarcoma, NOS; metastatic)
Relative Expression (log2-ratio):3.3236332Number of Samples:2 / 2
| Experimental | connective/soft tissue cancer study 1 (PDX; connective and soft tissue, leiomyosarcoma, NOS; metastatic) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from a metastasis of patients with primary connective and soft tissue, leiomyosarcoma, NOS of the soft tissue (subcutaneously implanted). Metastatic site of patient tumor sample is not reported. | |
| Control | connective/soft tissue cancer study 1 (PDX; connective and soft tissue, clear cell sarcoma, NOS; metastatic) |
| Patient-derived xenograft (PDX) samples generated in female athymic nude mice from a metastasis of patients with primary connective and soft tissue, clear cell sarcoma, NOS of the soft tissue (subcutaneously implanted). Metastatic site of patient tumor sample is not reported. |
wound healing study 2 (ex vivo; DMSO) / normal skin tissue (ex vivo)
Relative Expression (log2-ratio):-3.2548838Number of Samples:3 / 3
| Experimental | wound healing study 2 (ex vivo; DMSO) |
| Ex vivo skin samples obtained from healthy donors following reduction surgery of abdomen, and incubated in culture medium containing DMSO for 4 days. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection. Skin was sliced into 1x10 mm slices and incubated in keratinocyte medium for four days with 1:1000 fold dilution of DMSO. The cultivation was performed in serum-free keratinocyte medium supplemented with transferrin, hEGF (0.15 ng/mL), 0.5 mg/mL hydrocortisone, gentamicin, amphotericin B, and epinephrine but without insulin. | |
| Control | normal skin tissue (ex vivo) |
| Normal skin samples obtained from healthy donors following reduction surgery of abdomen. To make sure that mainly epidermis was present in the samples, as much dermal tissue as possible was removed by dissection. |