TOP TEN perturbations for 39145_at (Homo sapiens)
Organism: Homo sapiens
Gene: 39145_at
Selected probe(set): 201058_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 39145_at (201058_s_at) across 6674 perturbations tested by GENEVESTIGATOR:
glioma study 16 (LN-229) / normal astrocyte sample
Relative Expression (log2-ratio):-6.948457Number of Samples:2 / 3
Experimental | glioma study 16 (LN-229) |
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2. | |
Control | normal astrocyte sample |
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations. |
hepatocyte (ESC) / HepaRG
Relative Expression (log2-ratio):6.458479Number of Samples:8 / 12
Experimental | hepatocyte (ESC) |
Hepatocyte-like cells differentiated from embryonic stem cells (ESC) | |
Control | HepaRG |
Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code: |
prostate cancer study 1 (meta.) / benign prostate tissue
Relative Expression (log2-ratio):-6.239382Number of Samples:6 / 6
Experimental | prostate cancer study 1 (meta.) |
Metastatic prostate cancer samples. | |
Control | benign prostate tissue |
Benign prostate samples. |
prostate cancer study 1 (meta.) / prostate cancer study 1 (prim.)
Relative Expression (log2-ratio):-5.840376Number of Samples:6 / 7
Experimental | prostate cancer study 1 (meta.) |
Metastatic prostate cancer samples. | |
Control | prostate cancer study 1 (prim.) |
Primary prostate cancer samples. |
pancreatic islet study 3 (expanded; PPRF) / normal pancreatic islet sample
Relative Expression (log2-ratio):5.237505Number of Samples:2 / 7
Experimental | pancreatic islet study 3 (expanded; PPRF) |
Human pancreatic islets were isolated from a cadaveric donor. The population was 70% pure in mature islets, which was determined by dithizone staining. Islets cells were expanded according to Pharmaceutical Production Research Facility Protocol (PPRF) for 6 weeks. Expansion phase: Approximately 2,500 islet equivalents were seeded onto 75-cm2 fibronectin-coated tissue culture-treated flasks in a proprietary serum-free medium (PPRF medium). The serum-free PPRF medium was supplemented with 30% serum-free mesenchymal-conditioned medium. When the cell migration resulted in the formation of large-size colonies, the cells were harvested by trypsinization and replated into new tissue culture flasks. Once the cells reached near confluence (80–90%) in monolayer, they were trypsinized, counted, and subcultured at a density of 5,000 cells per cm2. Thereafter, cells were serially passaged using the same protocol. | |
Control | normal pancreatic islet sample |
Pancreatic islets were obtained from seven donors aged between 37 and 70 years and body mass index between 22 and 27. Functional islets were cultured in CMRL 1066 supplemented with 10% FCS, 1% glutamine, 5.6 mM glucose, 1 mM HEPES, 110 U/ml penicillin, and 110 g/ml streptomycin for 7 days or immediately processed after isolation. |
hepatocyte (ESC) / Hep-G2
Relative Expression (log2-ratio):5.2205133Number of Samples:8 / 9
Experimental | hepatocyte (ESC) |
Hepatocyte-like cells differentiated from embryonic stem cells (ESC) | |
Control | Hep-G2 |
Human primary cancer cell line derived from the liver of a patient with hepatocellular carcinoma. Synonyms:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 Cellosaurus code: |
prostate cancer study 8 (p. canc) / prostate cancer study 8 (psfmc)
Relative Expression (log2-ratio):-5.064598Number of Samples:3 / 5
Experimental | prostate cancer study 8 (p. canc) |
CD26+ FACS sorted prostate neoplasm cell (p. canc) samples from patients with primary prostate cancer collected after radical prostatectomy. | |
Control | prostate cancer study 8 (psfmc) |
CD49a+ FACS sorted prostate stromal fibromuscular cell (psfmc) samples from patients with primary prostate cancer collected after radical prostatectomy. |
bladder cancer study 1 / normal bladder tissue
Relative Expression (log2-ratio):-4.9760704Number of Samples:3 / 3
Experimental | bladder cancer study 1 |
Low grade superficial tumor samples. | |
Control | normal bladder tissue |
Normal bladder tissue. |
glioma study 16 (LN-319) / normal astrocyte sample
Relative Expression (log2-ratio):-4.6081133Number of Samples:2 / 3
Experimental | glioma study 16 (LN-319) |
Human glioma cell line LN319 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2. | |
Control | normal astrocyte sample |
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations. |
rapidly progressive glomerulonephritis; ANCA-associated vasculitis study 5 (tubulointerstitium) / focal segmental glomerulosclerosis study 9 (glomerulus)
Relative Expression (log2-ratio):-4.4088736Number of Samples:21 / 10
Experimental | rapidly progressive glomerulonephritis; ANCA-associated vasculitis study 5 (tubulointerstitium) |
Tubulointerstitium tissue sample obtained by microdissection from kidney biopsy, from patients suffering from rapidly progressive glomerulonephritis and antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Donors were positive for ANCA-antibody. Biopsy samples were obtained from the European Renal cDNA Bank-Kroener-Fresenius biopsy bank. All biopsies were stratified by a reference pathologist. | |
Control | focal segmental glomerulosclerosis study 9 (glomerulus) |
Glomeruli tissue sample obtained by microdissection from kidney biopsy, from patients suffering from focal segmental glomerulosclerosis. Biopsy samples were obtained from the European Renal cDNA Bank-Kroener-Fresenius biopsy bank. All biopsies were stratified by a reference pathologist. |