Organism: Homo sapiens
Gene: 39220_at
Selected probe(set): 205725_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39220_at (205725_at) across 6674 perturbations tested by GENEVESTIGATOR:

Relative Expression (log2-ratio):7.4521065
Number of Samples:3 / 3

Experimental	bronchial epithelial cell differentiation study 1 (day28)
	Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control	bronchial epithelial cell differentiation study 1 (subconfluent)
	Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

Relative Expression (log2-ratio):7.2229424
Number of Samples:3 / 3

Experimental	bronchial epithelial cell differentiation study 1 (day28)
	Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control	bronchial epithelial cell differentiation study 1 (confluent)
	Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert.

Relative Expression (log2-ratio):-6.457323
Number of Samples:9 / 3

Experimental	lung adenocarcinoma study 3 (negative)
	Primary lung adenocarcinoma samples, with bone marrow negative for early disseminated tumor cells (DTCs). The presence of DTCs can predict the subsequent occurrence of overt metastasis and survival in lung cancer.
Control	normal lung tissue
	Normal lung tissue.

Relative Expression (log2-ratio):-6.1081266
Number of Samples:7 / 3

Experimental	lung adenocarcinoma study 3 (positive)
	Primary lung adenocarcinoma samples, with bone marrow positive for early disseminated tumor cells (DTCs). The presence of DTCs can predict the subsequent occurrence of overt metastasis and survival in lung cancer.
Control	normal lung tissue
	Normal lung tissue.

Relative Expression (log2-ratio):-5.5074253
Number of Samples:6 / 13

Experimental	expO lung cancer study 1 (neuroendocrine carcinoma; primary)
	Primary tumor tissue samples obtained from the lung of patients with neuroendocrine carcinoma.
Control	expO lung cancer study 1 (bronchiolo-alveolar adenocarcinoma; primary)
	Primary tumor tissue samples obtained from the lung of patients with bronchiolo-alveolar adenocarcinoma.

Relative Expression (log2-ratio):-5.111166
Number of Samples:14 / 44

Experimental	lung adenocarcinoma study 4
	Primary lung adenocarcinoma samples of a patient with non-small cell lung cancer.
Control	normal lung tissue
	Histologically normal lung tissue.

Relative Expression (log2-ratio):-4.9158115
Number of Samples:18 / 65

Experimental	non-small cell lung cancer study 8 (large cell carcinoma)
	Primary tumor tissue sample obtained from the lung of patients large cell carcinoma.
Control	adjacent lung tissue
	Histological normal lung tissue samples from patients with lung cancer.

Relative Expression (log2-ratio):-4.47025
Number of Samples:2 / 13

Experimental	expO lung cancer study 1 (papillary adenocarcinoma, NOS; primary)
	Primary tumor tissue samples obtained from the lung of patients with papillary adenocarcinoma (NOS).
Control	expO lung cancer study 1 (bronchiolo-alveolar adenocarcinoma; primary)
	Primary tumor tissue samples obtained from the lung of patients with bronchiolo-alveolar adenocarcinoma.

Relative Expression (log2-ratio):4.456458
Number of Samples:3 / 3

Experimental	transwell differentiation study 1 (TEER base)
	MCF10A cells were plated onto polyester permeable supports (Transwells) at a density of 10^5 cells/cm^2. Base represents those plates with a low trans-epithelial electrical resistance (TEER) of 200-300 Ohms x cm^2.
Control	untreated MCF10A cell sample
	MCF10A cells grown to confluency on sterile plastic.

Relative Expression (log2-ratio):-4.202138
Number of Samples:3 / 20

Experimental	lung adenocarcinoma study 5 (EML4-ALK)
	Primary tumor sample from patients with stage I adenocarcinoma carrying the EML4-ALK-fusion.
Control	adjacent lung tissue
	Adjacent and histologically normal, non-tumorous lung tissue samples from patients with lung adenocarcinoma.