TOP TEN perturbations for 39220_at (Homo sapiens)

Organism: Homo sapiens
Gene: 39220_at
Selected probe(set): 205725_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39220_at (205725_at) across 6674 perturbations tested by GENEVESTIGATOR:

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):7.4521065
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)

Relative Expression (log2-ratio):7.2229424
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (confluent)
Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert.

lung adenocarcinoma study 3 (negative) / normal lung tissue

Relative Expression (log2-ratio):-6.457323
Number of Samples:9 / 3
Experimental lung adenocarcinoma study 3 (negative)
Primary lung adenocarcinoma samples, with bone marrow negative for early disseminated tumor cells (DTCs). The presence of DTCs can predict the subsequent occurrence of overt metastasis and survival in lung cancer.
Control normal lung tissue
Normal lung tissue.

lung adenocarcinoma study 3 (positive) / normal lung tissue

Relative Expression (log2-ratio):-6.1081266
Number of Samples:7 / 3
Experimental lung adenocarcinoma study 3 (positive)
Primary lung adenocarcinoma samples, with bone marrow positive for early disseminated tumor cells (DTCs). The presence of DTCs can predict the subsequent occurrence of overt metastasis and survival in lung cancer.
Control normal lung tissue
Normal lung tissue.

expO lung cancer study 1 (neuroendocrine carcinoma; primary) / expO lung cancer study 1 (bronchiolo-alveolar adenocarcinoma; primary)

Relative Expression (log2-ratio):-5.5074253
Number of Samples:6 / 13
Experimental expO lung cancer study 1 (neuroendocrine carcinoma; primary)
Primary tumor tissue samples obtained from the lung of patients with neuroendocrine carcinoma.
Control expO lung cancer study 1 (bronchiolo-alveolar adenocarcinoma; primary)
Primary tumor tissue samples obtained from the lung of patients with bronchiolo-alveolar adenocarcinoma.

lung adenocarcinoma study 4 / normal lung tissue

Relative Expression (log2-ratio):-5.111166
Number of Samples:14 / 44
Experimental lung adenocarcinoma study 4
Primary lung adenocarcinoma samples of a patient with non-small cell lung cancer.
Control normal lung tissue
Histologically normal lung tissue.

non-small cell lung cancer study 8 (large cell carcinoma) / adjacent lung tissue

Relative Expression (log2-ratio):-4.9158115
Number of Samples:18 / 65
Experimental non-small cell lung cancer study 8 (large cell carcinoma)
Primary tumor tissue sample obtained from the lung of patients large cell carcinoma.
Control adjacent lung tissue
Histological normal lung tissue samples from patients with lung cancer.

expO lung cancer study 1 (papillary adenocarcinoma, NOS; primary) / expO lung cancer study 1 (bronchiolo-alveolar adenocarcinoma; primary)

Relative Expression (log2-ratio):-4.47025
Number of Samples:2 / 13
Experimental expO lung cancer study 1 (papillary adenocarcinoma, NOS; primary)
Primary tumor tissue samples obtained from the lung of patients with papillary adenocarcinoma (NOS).
Control expO lung cancer study 1 (bronchiolo-alveolar adenocarcinoma; primary)
Primary tumor tissue samples obtained from the lung of patients with bronchiolo-alveolar adenocarcinoma.

transwell differentiation study 1 (TEER base) / untreated MCF10A cell sample

Relative Expression (log2-ratio):4.456458
Number of Samples:3 / 3
Experimental transwell differentiation study 1 (TEER base)
MCF10A cells were plated onto polyester permeable supports (Transwells) at a density of 10^5 cells/cm^2. Base represents those plates with a low trans-epithelial electrical resistance (TEER) of 200-300 Ohms x cm^2.
Control untreated MCF10A cell sample
MCF10A cells grown to confluency on sterile plastic.

lung adenocarcinoma study 5 (EML4-ALK) / adjacent lung tissue

Relative Expression (log2-ratio):-4.202138
Number of Samples:3 / 20
Experimental lung adenocarcinoma study 5 (EML4-ALK)
Primary tumor sample from patients with stage I adenocarcinoma carrying the EML4-ALK-fusion.
Control adjacent lung tissue
Adjacent and histologically normal, non-tumorous lung tissue samples from patients with lung adenocarcinoma.