TOP TEN perturbations for 39269_at (Homo sapiens)

Organism: Homo sapiens
Gene: 39269_at
Selected probe(set): 204127_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39269_at (204127_at) across 6431 perturbations tested by GENEVESTIGATOR:

colorectal cancer study 4 (recurring) / adjacent colon tissue (recurring)

Relative Expression (log2-ratio):4.571335
Number of Samples:24 / 3
Experimental colorectal cancer study 4 (recurring)
LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer. Patients developed metastatic recurrence during follow-up after surgery.
Control adjacent colon tissue (recurring)
Histologically normal colon tissue samples from patients with primary colorectal cancer collected after resection. Tissue was further extracted using laser-capture microdissection (LCM). With metastatic recurrence after resection during follow-up.

T-cell activation study 2 / quiescent CD4+ T-cell sample

Relative Expression (log2-ratio):3.6719294
Number of Samples:2 / 2
Experimental T-cell activation study 2
CD4+ T-cell samples derived from PBMC´s of HIV-seronegative donors were activated with plate bound CD3 (1ug/ml) and soluble CD28 (50ng/ml) for 2 days..
Control quiescent CD4+ T-cell sample
Quiescent CD4+ T-cell samples derived from PBMC´s of HIV-seronegative donors.

tumor supernatant activation study 3 / memory T-cell activation study 1

Relative Expression (log2-ratio):-3.5564585
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control memory T-cell activation study 1
Memory (CD45RA-) CD4+ T-cells isolated from peripheral blood of healthy female donors were activated with anti-CD3/CD28 beads for 24hrs.

azathioprine study 8 (48h) / vehicle (DMSO) treated HepG2 cell sample

Relative Expression (log2-ratio):-3.3798952
Number of Samples:2 / 19
Experimental azathioprine study 8 (48h)
HepG2 cells exposed to 250μM azathioprine in DMSO solvent for 48 hours. ATC code:
Control vehicle (DMSO) treated HepG2 cell sample
HepG2 cells exposed to DMSO solvent for 48 hours.

colorectal cancer study 4 (non-recurring) / adjacent colon tissue (non-recurring)

Relative Expression (log2-ratio):3.3760757
Number of Samples:76 / 18
Experimental colorectal cancer study 4 (non-recurring)
LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer. Patients did not develop metastatic recurrence during follow-up after surgery.
Control adjacent colon tissue (non-recurring)
Histologically normal colon tissue samples from patients with primary colorectal cancer collected after resection. Tissue was further extracted using laser-capture microdissection (LCM). No metastatic recurrence during follow-up.

colorectal cancer study 4 (metastatic progression) / adjacent colon tissue (metastatic progression)

Relative Expression (log2-ratio):3.3064213
Number of Samples:23 / 4
Experimental colorectal cancer study 4 (metastatic progression)
LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer with metastatic progression.
Control adjacent colon tissue (metastatic progression)
Histologically normal colon tissue samples from patients with primary colorectal cancer and metastatic progression collected after resection. Tissue was further extracted using laser-capture microdissection (LCM).

T-cell activation study 4 / normal resting T-cell sample

Relative Expression (log2-ratio):3.2831383
Number of Samples:2 / 2
Experimental T-cell activation study 4
T-cell samples antiCD3 activated for 30hrs.
Control normal resting T-cell sample
T cells resting for 30h.

colorectal cancer study 33 (carcinoma; trans. colon) / normal transverse colon tissue

Relative Expression (log2-ratio):3.1770792
Number of Samples:2 / 2
Experimental colorectal cancer study 33 (carcinoma; trans. colon)
Primary tumor samples derived from the transverse colon of patients with carcinoma.
Control normal transverse colon tissue
Normal transverse colon tissue samples.

CH5183284 study 1 (HSC-39) / vehicle (DMSO) treated HSC-39 cell sample

Relative Expression (log2-ratio):-3.108842
Number of Samples:2 / 2
Experimental CH5183284 study 1 (HSC-39)
Human gastric cell line HSC-39 treated with 1 uM of FGFR inhibitor CH5183284 for 24 hours. ATC code:---
Control vehicle (DMSO) treated HSC-39 cell sample
Human gastric cell line HSC-39 treated with 0.1% DMSO for 24 hours.

C. difficile study 1 (TcdA; 24h) / untreated HCT 8 cell sample

Relative Expression (log2-ratio):-3.084179
Number of Samples:2 / 2
Experimental C. difficile study 1 (TcdA; 24h)
HCT 8 cells were treated with 100 ng/ml Toxin A (TcdA) isolated from Clostridium difficile strain VPI-10643 in RPMI-1640 supplemented with 10% heat-inactivated fetal bovine serum and 1mM sodium pyruvate for 24 hours.
Control untreated HCT 8 cell sample
Untreated HCT 8 cell sample cultured in RPMI-1640 supplemented with 10% heat-inactivated fetal bovine serum and 1mM sodium pyruvate for 24 hours.