TOP TEN perturbations for 39420_at (Homo sapiens)
Organism: Homo sapiens
Gene: 39420_at
Selected probe(set): 209383_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 39420_at (209383_at) across 6674 perturbations tested by GENEVESTIGATOR:
Barrett's esophagus study 2 (doca) early / untreated CP-A hTERT cell sample
Relative Expression (log2-ratio):5.2323637Number of Samples:2 / 2
| Experimental | Barrett's esophagus study 2 (doca) early |
| CP-A hTERT Barrett's esophagus cells 2 hours after 15 minute exposure to deoxycholic acid, pH 4.5. | |
| Control | untreated CP-A hTERT cell sample |
| CP-A hTERT Barrett's esophagus cells unexposed. |
sapphyrin PCI-5002; ZnOAc2 study 1 / mannitol treated A549 cell sample
Relative Expression (log2-ratio):4.3614483Number of Samples:3 / 3
| Experimental | sapphyrin PCI-5002; ZnOAc2 study 1 |
| A549 human lung cancer cells were seeded 8 days. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. ATC code:--- | |
| Control | mannitol treated A549 cell sample |
| A549 human lung cancer cells were seeded 8 days. At 4 hours prior to RNA isolation, 5% mannitol solution was added to the cultures. |
brefeldin A study 1 (0.5ug/ml; HCT 116) / untreated HCT 116 cell sample
Relative Expression (log2-ratio):4.341117Number of Samples:3 / 3
| Experimental | brefeldin A study 1 (0.5ug/ml; HCT 116) |
| Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:--- | |
| Control | untreated HCT 116 cell sample |
| Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS. |
Barrett's esophagus study 2 (doca) late / untreated CP-A hTERT cell sample
Relative Expression (log2-ratio):4.1821775Number of Samples:2 / 2
| Experimental | Barrett's esophagus study 2 (doca) late |
| CP-A hTERT Barrett's esophagus cells 6 hours after 15 minute exposure to deoxycholic acid, pH 4.5. | |
| Control | untreated CP-A hTERT cell sample |
| CP-A hTERT Barrett's esophagus cells unexposed. |
brefeldin A study 1 (0.5ug/ml; HCT 116 DICER1(-/-)) / untreated HCT 116 DICER1(-/-) cell sample
Relative Expression (log2-ratio):4.068227Number of Samples:3 / 3
| Experimental | brefeldin A study 1 (0.5ug/ml; HCT 116 DICER1(-/-)) |
| Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:--- | |
| Control | untreated HCT 116 DICER1(-/-) cell sample |
| Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 by was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS. |
nonoxynol 9 study 1 (12.5ug/ml; 6h) / untreated VK2/E6E7 cell sample
Relative Expression (log2-ratio):3.9990387Number of Samples:9 / 54
| Experimental | nonoxynol 9 study 1 (12.5ug/ml; 6h) |
| Vaginal immortalized epithelial cell line Vk2/E6E7 treated with 12.5 ug/ml of nonoxynol 9 for 6 hours. ATC code:--- | |
| Control | untreated VK2/E6E7 cell sample |
| Untreated vaginal immortalized epithelial cell line Vk2/E6E7 maintained in keratinocyte serum-free medium for 6 hours |
smoking study 66 (4h) / normal sham-exposed nasal organotypic tissue
Relative Expression (log2-ratio):3.862053Number of Samples:3 / 3
| Experimental | smoking study 66 (4h) |
| Human nasal organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 4 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male. | |
| Control | normal sham-exposed nasal organotypic tissue |
| Human nasal organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 4 hours. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male. |
catechol study 1 (3300ug/ml) / vehicle (EtOH) treated bronchial epithelial cell sample
Relative Expression (log2-ratio):3.68005Number of Samples:3 / 3
| Experimental | catechol study 1 (3300ug/ml) |
| Bronchial epithelial cells (NHBE) treated with 3300 ug/ml catechol (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 4 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:--- | |
| Control | vehicle (EtOH) treated bronchial epithelial cell sample |
| Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 4 hours. NHBE cells were derived from a 60 year old male non-smoker. |
tetracycline study 7 (2000uM) / vehicle (DMSO) treated HepG2 sample
Relative Expression (log2-ratio):3.6082478Number of Samples:3 / 21
| Experimental | tetracycline study 7 (2000uM) |
| HepG2 cells treated with compound: tetracycline (2000uM; CAS no.:60-54-8) for 24 hours. Tetracycline is hepatotoxic and may cause steatosis. HepG2 cells were treated with the IC20 concentration measured after 24 hours. ATC code:, , , , , | |
| Control | vehicle (DMSO) treated HepG2 sample |
| HepG2 cells treated with DMSO (0.5% v/v) as solvent control for 24 hours. |
smoking study 55 (4 hrs) / normal sham-exposed bronchial epithelial cell sample
Relative Expression (log2-ratio):3.5837202Number of Samples:3 / 3
| Experimental | smoking study 55 (4 hrs) |
| Bronchial epithelium organotypic tissue culture harvested 4 hours after 4 repetitive exposures to 16% (vol/vol) mainstream cigarette smoke (one 3R4F reference cigarette pro exposure), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts. | |
| Control | normal sham-exposed bronchial epithelial cell sample |
| Bronchial epithelium organotypic tissue culture harvested 4 hours after 4 single exposures to 100% humidified air (with 60% humidity), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts. |
Organism: Homo sapiens
Gene: 39420_at
Selected probe(set): 209383_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 39420_at (209383_at) across 6674 perturbations tested by GENEVESTIGATOR:
Barrett's esophagus study 2 (doca) early / untreated CP-A hTERT cell sample
Relative Expression (log2-ratio):5.2323637Number of Samples:2 / 2
| Experimental | Barrett's esophagus study 2 (doca) early |
| CP-A hTERT Barrett's esophagus cells 2 hours after 15 minute exposure to deoxycholic acid, pH 4.5. | |
| Control | untreated CP-A hTERT cell sample |
| CP-A hTERT Barrett's esophagus cells unexposed. |
sapphyrin PCI-5002; ZnOAc2 study 1 / mannitol treated A549 cell sample
Relative Expression (log2-ratio):4.3614483Number of Samples:3 / 3
| Experimental | sapphyrin PCI-5002; ZnOAc2 study 1 |
| A549 human lung cancer cells were seeded 8 days. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. ATC code:--- | |
| Control | mannitol treated A549 cell sample |
| A549 human lung cancer cells were seeded 8 days. At 4 hours prior to RNA isolation, 5% mannitol solution was added to the cultures. |
brefeldin A study 1 (0.5ug/ml; HCT 116) / untreated HCT 116 cell sample
Relative Expression (log2-ratio):4.341117Number of Samples:3 / 3
| Experimental | brefeldin A study 1 (0.5ug/ml; HCT 116) |
| Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:--- | |
| Control | untreated HCT 116 cell sample |
| Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS. |
Barrett's esophagus study 2 (doca) late / untreated CP-A hTERT cell sample
Relative Expression (log2-ratio):4.1821775Number of Samples:2 / 2
| Experimental | Barrett's esophagus study 2 (doca) late |
| CP-A hTERT Barrett's esophagus cells 6 hours after 15 minute exposure to deoxycholic acid, pH 4.5. | |
| Control | untreated CP-A hTERT cell sample |
| CP-A hTERT Barrett's esophagus cells unexposed. |
brefeldin A study 1 (0.5ug/ml; HCT 116 DICER1(-/-)) / untreated HCT 116 DICER1(-/-) cell sample
Relative Expression (log2-ratio):4.068227Number of Samples:3 / 3
| Experimental | brefeldin A study 1 (0.5ug/ml; HCT 116 DICER1(-/-)) |
| Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:--- | |
| Control | untreated HCT 116 DICER1(-/-) cell sample |
| Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 by was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS. |
nonoxynol 9 study 1 (12.5ug/ml; 6h) / untreated VK2/E6E7 cell sample
Relative Expression (log2-ratio):3.9990387Number of Samples:9 / 54
| Experimental | nonoxynol 9 study 1 (12.5ug/ml; 6h) |
| Vaginal immortalized epithelial cell line Vk2/E6E7 treated with 12.5 ug/ml of nonoxynol 9 for 6 hours. ATC code:--- | |
| Control | untreated VK2/E6E7 cell sample |
| Untreated vaginal immortalized epithelial cell line Vk2/E6E7 maintained in keratinocyte serum-free medium for 6 hours |
smoking study 66 (4h) / normal sham-exposed nasal organotypic tissue
Relative Expression (log2-ratio):3.862053Number of Samples:3 / 3
| Experimental | smoking study 66 (4h) |
| Human nasal organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 4 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male. | |
| Control | normal sham-exposed nasal organotypic tissue |
| Human nasal organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 4 hours. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male. |
catechol study 1 (3300ug/ml) / vehicle (EtOH) treated bronchial epithelial cell sample
Relative Expression (log2-ratio):3.68005Number of Samples:3 / 3
| Experimental | catechol study 1 (3300ug/ml) |
| Bronchial epithelial cells (NHBE) treated with 3300 ug/ml catechol (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 4 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:--- | |
| Control | vehicle (EtOH) treated bronchial epithelial cell sample |
| Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 4 hours. NHBE cells were derived from a 60 year old male non-smoker. |
tetracycline study 7 (2000uM) / vehicle (DMSO) treated HepG2 sample
Relative Expression (log2-ratio):3.6082478Number of Samples:3 / 21
| Experimental | tetracycline study 7 (2000uM) |
| HepG2 cells treated with compound: tetracycline (2000uM; CAS no.:60-54-8) for 24 hours. Tetracycline is hepatotoxic and may cause steatosis. HepG2 cells were treated with the IC20 concentration measured after 24 hours. ATC code:, , , , , | |
| Control | vehicle (DMSO) treated HepG2 sample |
| HepG2 cells treated with DMSO (0.5% v/v) as solvent control for 24 hours. |
smoking study 55 (4 hrs) / normal sham-exposed bronchial epithelial cell sample
Relative Expression (log2-ratio):3.5837202Number of Samples:3 / 3
| Experimental | smoking study 55 (4 hrs) |
| Bronchial epithelium organotypic tissue culture harvested 4 hours after 4 repetitive exposures to 16% (vol/vol) mainstream cigarette smoke (one 3R4F reference cigarette pro exposure), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts. | |
| Control | normal sham-exposed bronchial epithelial cell sample |
| Bronchial epithelium organotypic tissue culture harvested 4 hours after 4 single exposures to 100% humidified air (with 60% humidity), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts. |