TOP TEN perturbations for 39420_at (Homo sapiens)

Organism: Homo sapiens
Gene: 39420_at
Selected probe(set): 209383_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39420_at (209383_at) across 6674 perturbations tested by GENEVESTIGATOR:

Barrett's esophagus study 2 (doca) early / untreated CP-A hTERT cell sample

Relative Expression (log2-ratio):5.2323637
Number of Samples:2 / 2
Experimental Barrett's esophagus study 2 (doca) early
CP-A hTERT Barrett's esophagus cells 2 hours after 15 minute exposure to deoxycholic acid, pH 4.5.
Control untreated CP-A hTERT cell sample
CP-A hTERT Barrett's esophagus cells unexposed.

sapphyrin PCI-5002; ZnOAc2 study 1 / mannitol treated A549 cell sample

Relative Expression (log2-ratio):4.3614483
Number of Samples:3 / 3
Experimental sapphyrin PCI-5002; ZnOAc2 study 1
A549 human lung cancer cells were seeded 8 days. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. ATC code:---
Control mannitol treated A549 cell sample
A549 human lung cancer cells were seeded 8 days. At 4 hours prior to RNA isolation, 5% mannitol solution was added to the cultures.

brefeldin A study 1 (0.5ug/ml; HCT 116) / untreated HCT 116 cell sample

Relative Expression (log2-ratio):4.341117
Number of Samples:3 / 3
Experimental brefeldin A study 1 (0.5ug/ml; HCT 116)
Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 cell sample
Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

Barrett's esophagus study 2 (doca) late / untreated CP-A hTERT cell sample

Relative Expression (log2-ratio):4.1821775
Number of Samples:2 / 2
Experimental Barrett's esophagus study 2 (doca) late
CP-A hTERT Barrett's esophagus cells 6 hours after 15 minute exposure to deoxycholic acid, pH 4.5.
Control untreated CP-A hTERT cell sample
CP-A hTERT Barrett's esophagus cells unexposed.

brefeldin A study 1 (0.5ug/ml; HCT 116 DICER1(-/-)) / untreated HCT 116 DICER1(-/-) cell sample

Relative Expression (log2-ratio):4.068227
Number of Samples:3 / 3
Experimental brefeldin A study 1 (0.5ug/ml; HCT 116 DICER1(-/-))
Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 DICER1(-/-) cell sample
Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 by was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

nonoxynol 9 study 1 (12.5ug/ml; 6h) / untreated VK2/E6E7 cell sample

Relative Expression (log2-ratio):3.9990387
Number of Samples:9 / 54
Experimental nonoxynol 9 study 1 (12.5ug/ml; 6h)
Vaginal immortalized epithelial cell line Vk2/E6E7 treated with 12.5 ug/ml of nonoxynol 9 for 6 hours. ATC code:---
Control untreated VK2/E6E7 cell sample
Untreated vaginal immortalized epithelial cell line Vk2/E6E7 maintained in keratinocyte serum-free medium for 6 hours

smoking study 66 (4h) / normal sham-exposed nasal organotypic tissue

Relative Expression (log2-ratio):3.862053
Number of Samples:3 / 3
Experimental smoking study 66 (4h)
Human nasal organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 4 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed nasal organotypic tissue
Human nasal organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 4 hours. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

catechol study 1 (3300ug/ml) / vehicle (EtOH) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):3.68005
Number of Samples:3 / 3
Experimental catechol study 1 (3300ug/ml)
Bronchial epithelial cells (NHBE) treated with 3300 ug/ml catechol (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 4 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:---
Control vehicle (EtOH) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 4 hours. NHBE cells were derived from a 60 year old male non-smoker.

tetracycline study 7 (2000uM) / vehicle (DMSO) treated HepG2 sample

Relative Expression (log2-ratio):3.6082478
Number of Samples:3 / 21
Experimental tetracycline study 7 (2000uM)
HepG2 cells treated with compound: tetracycline (2000uM; CAS no.:60-54-8) for 24 hours. Tetracycline is hepatotoxic and may cause steatosis. HepG2 cells were treated with the IC20 concentration measured after 24 hours. ATC code:, , , , ,
Control vehicle (DMSO) treated HepG2 sample
HepG2 cells treated with DMSO (0.5% v/v) as solvent control for 24 hours.

smoking study 55 (4 hrs) / normal sham-exposed bronchial epithelial cell sample

Relative Expression (log2-ratio):3.5837202
Number of Samples:3 / 3
Experimental smoking study 55 (4 hrs)
Bronchial epithelium organotypic tissue culture harvested 4 hours after 4 repetitive exposures to 16% (vol/vol) mainstream cigarette smoke (one 3R4F reference cigarette pro exposure), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts.
Control normal sham-exposed bronchial epithelial cell sample
Bronchial epithelium organotypic tissue culture harvested 4 hours after 4 single exposures to 100% humidified air (with 60% humidity), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts.

Organism: Homo sapiens
Gene: 39420_at
Selected probe(set): 209383_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39420_at (209383_at) across 6674 perturbations tested by GENEVESTIGATOR:

Barrett's esophagus study 2 (doca) early / untreated CP-A hTERT cell sample

Relative Expression (log2-ratio):5.2323637
Number of Samples:2 / 2
Experimental Barrett's esophagus study 2 (doca) early
CP-A hTERT Barrett's esophagus cells 2 hours after 15 minute exposure to deoxycholic acid, pH 4.5.
Control untreated CP-A hTERT cell sample
CP-A hTERT Barrett's esophagus cells unexposed.

sapphyrin PCI-5002; ZnOAc2 study 1 / mannitol treated A549 cell sample

Relative Expression (log2-ratio):4.3614483
Number of Samples:3 / 3
Experimental sapphyrin PCI-5002; ZnOAc2 study 1
A549 human lung cancer cells were seeded 8 days. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. ATC code:---
Control mannitol treated A549 cell sample
A549 human lung cancer cells were seeded 8 days. At 4 hours prior to RNA isolation, 5% mannitol solution was added to the cultures.

brefeldin A study 1 (0.5ug/ml; HCT 116) / untreated HCT 116 cell sample

Relative Expression (log2-ratio):4.341117
Number of Samples:3 / 3
Experimental brefeldin A study 1 (0.5ug/ml; HCT 116)
Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 cell sample
Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

Barrett's esophagus study 2 (doca) late / untreated CP-A hTERT cell sample

Relative Expression (log2-ratio):4.1821775
Number of Samples:2 / 2
Experimental Barrett's esophagus study 2 (doca) late
CP-A hTERT Barrett's esophagus cells 6 hours after 15 minute exposure to deoxycholic acid, pH 4.5.
Control untreated CP-A hTERT cell sample
CP-A hTERT Barrett's esophagus cells unexposed.

brefeldin A study 1 (0.5ug/ml; HCT 116 DICER1(-/-)) / untreated HCT 116 DICER1(-/-) cell sample

Relative Expression (log2-ratio):4.068227
Number of Samples:3 / 3
Experimental brefeldin A study 1 (0.5ug/ml; HCT 116 DICER1(-/-))
Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 DICER1(-/-) cell sample
Derived human colon carcinoma cell line HCT 116 DICER1(-/-) with knockout DICER gene in exon 5 by was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

nonoxynol 9 study 1 (12.5ug/ml; 6h) / untreated VK2/E6E7 cell sample

Relative Expression (log2-ratio):3.9990387
Number of Samples:9 / 54
Experimental nonoxynol 9 study 1 (12.5ug/ml; 6h)
Vaginal immortalized epithelial cell line Vk2/E6E7 treated with 12.5 ug/ml of nonoxynol 9 for 6 hours. ATC code:---
Control untreated VK2/E6E7 cell sample
Untreated vaginal immortalized epithelial cell line Vk2/E6E7 maintained in keratinocyte serum-free medium for 6 hours

smoking study 66 (4h) / normal sham-exposed nasal organotypic tissue

Relative Expression (log2-ratio):3.862053
Number of Samples:3 / 3
Experimental smoking study 66 (4h)
Human nasal organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 4 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed nasal organotypic tissue
Human nasal organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 4 hours. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.

catechol study 1 (3300ug/ml) / vehicle (EtOH) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):3.68005
Number of Samples:3 / 3
Experimental catechol study 1 (3300ug/ml)
Bronchial epithelial cells (NHBE) treated with 3300 ug/ml catechol (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 4 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:---
Control vehicle (EtOH) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 4 hours. NHBE cells were derived from a 60 year old male non-smoker.

tetracycline study 7 (2000uM) / vehicle (DMSO) treated HepG2 sample

Relative Expression (log2-ratio):3.6082478
Number of Samples:3 / 21
Experimental tetracycline study 7 (2000uM)
HepG2 cells treated with compound: tetracycline (2000uM; CAS no.:60-54-8) for 24 hours. Tetracycline is hepatotoxic and may cause steatosis. HepG2 cells were treated with the IC20 concentration measured after 24 hours. ATC code:, , , , ,
Control vehicle (DMSO) treated HepG2 sample
HepG2 cells treated with DMSO (0.5% v/v) as solvent control for 24 hours.

smoking study 55 (4 hrs) / normal sham-exposed bronchial epithelial cell sample

Relative Expression (log2-ratio):3.5837202
Number of Samples:3 / 3
Experimental smoking study 55 (4 hrs)
Bronchial epithelium organotypic tissue culture harvested 4 hours after 4 repetitive exposures to 16% (vol/vol) mainstream cigarette smoke (one 3R4F reference cigarette pro exposure), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts.
Control normal sham-exposed bronchial epithelial cell sample
Bronchial epithelium organotypic tissue culture harvested 4 hours after 4 single exposures to 100% humidified air (with 60% humidity), with 1 hour rest between each treatment. Exposure was performed directly at the air/liquid interface and culture medium was replaced immediately after treatment. The 3D tissue model was generated from primary human respiratory epithelial cells derived from a 67 years old Caucasian non-smoking female donor co-cultured with primary human airway fibroblasts.