TOP TEN perturbations for 39450_s_at (Homo sapiens)
Organism: Homo sapiens
Gene: 39450_s_at
Selected probe(set): 210666_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 39450_s_at (210666_at) across 6674 perturbations tested by GENEVESTIGATOR:
T-cell activation study 3 / resting CD4 T-lymphocyte (crude fraction) sample
Relative Expression (log2-ratio):-2.548685Number of Samples:2 / 2
| Experimental | T-cell activation study 3 |
| CD4+ T-cell samples prepared from crude lymphocyte fraction. Cells were activated with anti-CD3/28 beads for 18hrs. | |
| Control | resting CD4 T-lymphocyte (crude fraction) sample |
| Resting CD4 T-lymphocytes prepared from crude lymphocyte fraction. |
pediatric septic shock study 3 (toddler; subclass C) / pediatric septic shock study 3 (toddler)
Relative Expression (log2-ratio):2.204504Number of Samples:14 / 23
| Experimental | pediatric septic shock study 3 (toddler; subclass C) |
| Whole blood samples obtained from toddlers (2 – 5 years) with septic shock subclass C. The samples were obtained within 24 hours of admission to the pediatric intensive care unit. One child did not survive. The subclass C was defined based on an empiric, discovery oriented expression filter and unsupervised hierarchical clustering. Patients from subclass C (when all age groups were pooled) had an illness severity level (PRISM III score) 15 (intra-quartile range (IQR) 10.7 - 19.2), maximum number of organ failures 2 (IQR 2 - 2), and a low mortality rate. | |
| Control | pediatric septic shock study 3 (toddler) |
| Whole blood obtained from toddlers (2 – 5 years) with septic shock. The samples were obtained within 24 hours of admission to the pediatric intensive care unit (day 1). Two children did not survive. |
pediatric septic shock study 3 (infant; subclass B) / pediatric septic shock study 3 (infant)
Relative Expression (log2-ratio):1.8525391Number of Samples:13 / 30
| Experimental | pediatric septic shock study 3 (infant; subclass B) |
| Whole blood samples obtained from infants (1 month – 1 year) with septic shock subclass B. The samples were obtained within 24 hours of admission to the pediatric intensive care unit. One child did not survive. The subclass B (when all age groups were pooled) was defined based on an empiric, discovery oriented expression filter and unsupervised hierarchical clustering. Patients in subclass B had an illness severity level (PRISM III score) 15 (intra-quartile range (IQR) 10.0 - 21.0), maximum number of organ failures 2 (IQR 2 - 3), and an intermediate mortality rate. A significantly greater proportion of patients in subclass B received hydrocortisone for cardiovascular shock. | |
| Control | pediatric septic shock study 3 (infant) |
| Whole blood obtained from infants (1 month – 1 year) with septic shock. The samples were obtained within 24 hours of admission to the pediatric intensive care unit (day 1). Five children did not survive. |
conditioned medium study 1 (HS27a) / untreated monocyte (CD14+) sample
Relative Expression (log2-ratio):-1.6969709Number of Samples:2 / 2
| Experimental | conditioned medium study 1 (HS27a) |
| CD14+ monocytes treated with HS27a conditioned medium (CM) for 48h. | |
| Control | untreated monocyte (CD14+) sample |
| Untreated CD14+ monocytes from two different donors. |
conditioned medium study 1 (HS5) / untreated monocyte (CD14+) sample
Relative Expression (log2-ratio):-1.6922646Number of Samples:2 / 2
| Experimental | conditioned medium study 1 (HS5) |
| CD14+ monocytes treated with HS5 conditioned medium (CM) for 48h. | |
| Control | untreated monocyte (CD14+) sample |
| Untreated CD14+ monocytes from two different donors. |
pediatric septic shock study 3 (infant; subclass C) / pediatric septic shock study 3 (infant)
Relative Expression (log2-ratio):1.6832256Number of Samples:9 / 30
| Experimental | pediatric septic shock study 3 (infant; subclass C) |
| Whole blood samples obtained from infants (1 month – 1 year) with septic shock subclass C. The samples were obtained within 24 hours of admission to the pediatric intensive care unit. All children survived. The subclass C was defined based on an empiric, discovery oriented expression filter and unsupervised hierarchical clustering. Patients from subclass C (when all age groups were pooled) had an illness severity level (PRISM III score) 15 (intra-quartile range (IQR) 10.7 - 19.2), maximum number of organ failures 2 (IQR 2 - 2), and a low mortality rate. | |
| Control | pediatric septic shock study 3 (infant) |
| Whole blood obtained from infants (1 month – 1 year) with septic shock. The samples were obtained within 24 hours of admission to the pediatric intensive care unit (day 1). Five children did not survive. |
pediatric septic shock study 3 (school-age; subclass B) / pediatric septic shock study 3 (school-age)
Relative Expression (log2-ratio):1.6789236Number of Samples:17 / 22
| Experimental | pediatric septic shock study 3 (school-age; subclass B) |
| Whole blood samples obtained from school-age children (≥ 6 years) with septic shock subclass B. The samples were obtained within 24 hours of admission to the pediatric intensive care unit. Two children did not survive. The subclass B was defined based on an empiric, discovery oriented expression filter and unsupervised hierarchical clustering. Patients in subclass B (when all age groups were pooled) had an illness severity level (PRISM III score) 15 (intra-quartile range (IQR) 10.0 - 21.0), maximum number of organ failures 2 (IQR 2 - 3), and an intermediate mortality rate. A significantly greater proportion of patients in subclass B received hydrocortisone for cardiovascular shock. | |
| Control | pediatric septic shock study 3 (school-age) |
| Whole blood obtained from scholar age children (> 6 years of age) with septic shock. The samples were obtained within 24 hours of admission to the pediatric intensive care unit. To determine survival rate, patients were followed for 28 days. |
pediatric septic shock study 3 (toddler; subclass B) / pediatric septic shock study 3 (toddler)
Relative Expression (log2-ratio):1.6632872Number of Samples:15 / 23
| Experimental | pediatric septic shock study 3 (toddler; subclass B) |
| Whole blood samples obtained from toddlers (2 – 5 years) with septic shock subclass B. The samples were obtained within 24 hours of admission to the pediatric intensive care unit. Two children did not survive. The subclass B was defined based on an empiric, discovery oriented expression filter and unsupervised hierarchical clustering. Patients in subclass B (when all age groups were pooled) had an illness severity level (PRISM III score) 15 (intra-quartile range (IQR) 10.0 - 21.0), maximum number of organ failures 2 (IQR 2 - 3), and an intermediate mortality rate. A significantly greater proportion of patients in subclass B received hydrocortisone for cardiovascular shock. | |
| Control | pediatric septic shock study 3 (toddler) |
| Whole blood obtained from toddlers (2 – 5 years) with septic shock. The samples were obtained within 24 hours of admission to the pediatric intensive care unit (day 1). Two children did not survive. |
pediatric septic shock study 3 (toddler; subclass C) / normal blood sample (toddler)
Relative Expression (log2-ratio):1.6204672Number of Samples:14 / 18
| Experimental | pediatric septic shock study 3 (toddler; subclass C) |
| Whole blood samples obtained from toddlers (2 – 5 years) with septic shock subclass C. The samples were obtained within 24 hours of admission to the pediatric intensive care unit. One child did not survive. The subclass C was defined based on an empiric, discovery oriented expression filter and unsupervised hierarchical clustering. Patients from subclass C (when all age groups were pooled) had an illness severity level (PRISM III score) 15 (intra-quartile range (IQR) 10.7 - 19.2), maximum number of organ failures 2 (IQR 2 - 2), and a low mortality rate. | |
| Control | normal blood sample (toddler) |
| Whole blood samples from toddlers (2 – 5 years). Children who had a recent febrile illness (within 2 weeks), who recently used anti-inflammatory medications (within 2 weeks) or who had any history of chronic or acute disease associated with inflammation were excluded from the study. |
follicular lymphoma study 1 / tonsillar CD4 T-cell sample
Relative Expression (log2-ratio):1.474678Number of Samples:12 / 7
| Experimental | follicular lymphoma study 1 |
| CD4+ tumor infiltrating T-cells were isolated by FACS from cryopreserved single cell suspension of lymph node biopsies from treatment naive patients with follicular lymphoma. The cells were characterized as CD3+ / CD4+ / CD19-, with > 96% purity and < 2% B-cell contamination. | |
| Control | tonsillar CD4 T-cell sample |
| CD4+ tonsillar T-cells were isolated by FACS from a single cell suspension from reactive tonsils. The cells were characterized as CD3+ / CD4+ / CD19-, with > 96% purity and < 2% B-cell contamination. |