TOP TEN perturbations for 39622_at (Homo sapiens)

Organism: Homo sapiens
Gene: 39622_at
Selected probe(set): 206269_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39622_at (206269_at) across 6674 perturbations tested by GENEVESTIGATOR:

tumor supernatant activation study 3 / memory T-cell activation study 1

Relative Expression (log2-ratio):4.506267
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control memory T-cell activation study 1
Memory (CD45RA-) CD4+ T-cells isolated from peripheral blood of healthy female donors were activated with anti-CD3/CD28 beads for 24hrs.

tumor supernatant activation study 3 / untreated memory CD4 T-cell sample

Relative Expression (log2-ratio):4.352758
Number of Samples:4 / 3
Experimental tumor supernatant activation study 3
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control untreated memory CD4 T-cell sample
Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

asthma study 22 (neutrophilic AIP) / asthma study 22 (paucigranulocytic AIP)

Relative Expression (log2-ratio):3.010684
Number of Samples:17 / 10
Experimental asthma study 22 (neutrophilic AIP)
Sputum samples from asthmatic patients with neutrophilic airway inflammatory phenotype (AIP).
Control asthma study 22 (paucigranulocytic AIP)
Sputum samples from asthmatic patients with paucigranulocytic airway inflammatory phenotype (AIP).

B-CLL study 11 (rolipram) / rolipram study 4 (normal B-cell; 20uM)

Relative Expression (log2-ratio):-2.7672052
Number of Samples:4 / 4
Experimental B-CLL study 11 (rolipram)
Peripheral blood mononuclear cells (PBMCs) obtained from chronic lymphocytic leukemia (CLL) patients and in vitro treated with rolipram (20 uM, cyclic nucleotide phosphodiesterase (PDE4) inhibitor) for 4 hours. PBMCs’ samples contained 90% CD19+ CD5+ B lineage CLL cells (B-CLL). Inclusion criteria: untreated CLL patients or at least 1 month after chemotherapy. Exclusion criteria: patients with active infections or other serious medical conditions or with white blood cell (WBC) counts of 15,000/l. ATC code:---
Control rolipram study 4 (normal B-cell; 20uM)
MACS purified resting B-cells from healthy donor peripheral blood treated with rolipram (20 uM, cyclic nucleotide phosphodiesterase (PDE4) inhibitor) for 4 hours. ATC code:---

asthma study 22 (eosinophilic AIP) / asthma study 22 (neutrophilic AIP)

Relative Expression (log2-ratio):-2.545826
Number of Samples:15 / 17
Experimental asthma study 22 (eosinophilic AIP)
Sputum samples from asthmatic patients with eosinophilic airway inflammatory phenotype (AIP).
Control asthma study 22 (neutrophilic AIP)
Sputum samples from asthmatic patients with neutrophilic airway inflammatory phenotype (AIP).

acrolein study 2 (2800ug/ml) / vehicle (EtOH) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):2.5223293
Number of Samples:3 / 3
Experimental acrolein study 2 (2800ug/ml)
Bronchial epithelial cells (NHBE) treated with 2800 ug/ml acrolein (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 8 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:---
Control vehicle (EtOH) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 8 hours. NHBE cells were derived from a 60 year old male non-smoker.

CVID study 1 (anergic B-cell) / rheumatoid arthritis study 18 (anergic B-cell)

Relative Expression (log2-ratio):2.500928
Number of Samples:3 / 2
Experimental CVID study 1 (anergic B-cell)
Anergic naive B-cells (CD19+,CD10-, CD21-/lo, CD27-) from patients suffering from Common Variable Immunodeficiency Disease (CVID).
Control rheumatoid arthritis study 18 (anergic B-cell)
Anergic naive B-cells (CD19+,CD10-, CD21-/lo, CD27-) from patients suffering from rheumatoid arthritis (RA) affecting multiple (subj.ID:19) or all (subj.ID:1) joints.

B-CLL study 11 (DMSO) / vehicle (DMSO) treated normal B-cell sample

Relative Expression (log2-ratio):-2.4602652
Number of Samples:4 / 4
Experimental B-CLL study 11 (DMSO)
Peripheral blood mononuclear cells (PBMCs) obtained from chronic lymphocytic leukemia (CLL) patients and in vitro treated with vehicle (DMSO) for 4 hours. PBMCs’ samples contained 90% CD19+ CD5+ B lineage CLL cells (B-CLL). Inclusion criteria: untreated CLL patients or at least 1 month after chemotherapy. Exclusion criteria: patients with active infections or other serious medical conditions or with white blood cell (WBC) counts of 15,000/l.
Control vehicle (DMSO) treated normal B-cell sample
MACS purified resting B-cells from healthy donor peripheral blood treated with vehicle (DMSO) for 4 hours.

diabetes type 2 study 27 (LCM) / diabetes type 2 study 27 (enzymatic)

Relative Expression (log2-ratio):-2.3394966
Number of Samples:34 / 19
Experimental diabetes type 2 study 27 (LCM)
Pancreatic islet samples obtained from type 2 diabetic (T2D) phenotyped pancreatectomized patients (PPP) and isolated by laser capture microdissection (LCM). Islets specimens were retrieved by LCM from snap-frozen surgical specimen from patients who underwent pancreatectomy for pancreatic diseases. Histopathology of the resected tissue did not reveal insulitis in any PPP. Patients age <18 years were excluded. Patients with type 2 diabetes had fasting glycemia ≥7.0 mmol/l; HbA1C ≥6.5% and history of diabetes for >1 year.
Control diabetes type 2 study 27 (enzymatic)
Pancreatic islet samples obtained from type 2 diabetic (T2D) patients and isolated by enzymatic digestion. Well-preserved islets were isolated by collagenase digestion of pancreas from brain-dead organ donors which suffered from type 2 diabetes. After 2±1 days of culture, islets were successfully hand-picked and processed for further analyses. Type 2 diabetes was diagnosed based on clinical history, treatment with glucose-lowering drugs, and lack of anti-GAD65 autoantibodies.

smoking study 66 (4h) / normal sham-exposed nasal organotypic tissue

Relative Expression (log2-ratio):2.3391395
Number of Samples:3 / 3
Experimental smoking study 66 (4h)
Human nasal organotypic tissue culture exposed at the air-liquid interface to 16.7% (vol/vol) mainstream cigarette smoke of 4 cigarettes (3R4F, 6-7 minutes each) followed by fresh culture medium incubation for 4 hours. Cigarettes were smoked on a 30-port carousel smoking machine according to health Canada regimen with 1 hour rest between each cigarette. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.
Control normal sham-exposed nasal organotypic tissue
Human nasal organotypic tissue culture exposed at the air-liquid interface to 60% humidified air (4 exposure with 1 hour rest between each treatment) followed by fresh culture medium incubation for 4 hours. Organotypic culture consisted in a co-culture of primary nasal respiratory epithelial cells derived from a healthy nonsmoking 65-year-old Caucasian male and primary airway fibroblasts derived from a healthy nonsmoking 61-year-old Caucasian male.