TOP TEN perturbations for 39670_at (Homo sapiens)

Organism: Homo sapiens
Gene: 39670_at
Selected probe(set): 209940_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39670_at (209940_at) across 6674 perturbations tested by GENEVESTIGATOR:

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):2.1790571
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):2.0955057
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37°C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):2.012267
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

IL-1b; IFN-g study 1 (72hrs) / untreated pancreatic islet sample (72hrs)

Relative Expression (log2-ratio):1.9052429
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (72hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 72 hours. Media were changed after 2 days.
Control untreated pancreatic islet sample (72hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 72 hours. Media were changed after 2 days.

IL-1b; IFN-g study 1 (84hrs) / untreated pancreatic islet sample (84hrs)

Relative Expression (log2-ratio):1.7236986
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (84hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 84 hours. Media were changed after 2 days.
Control untreated pancreatic islet sample (84hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 84 hours. Media were changed after 2 days.

IL-1b; IFN-g study 1 (108hrs) / untreated pancreatic islet sample (108hrs)

Relative Expression (log2-ratio):1.6831436
Number of Samples:2 / 2
Experimental IL-1b; IFN-g study 1 (108hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 108 hours. Media were changed after 2 and 5 days.
Control untreated pancreatic islet sample (108hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 108 hours. Media were changed after 2 and 5 days.

OPM-1 / MM1.S

Relative Expression (log2-ratio):-1.6255198
Number of Samples:4 / 4
Experimental OPM-1
Human primary cancer cell line derived from the peripheral blood of a patient with multiple myeloma. Dexamethasone-resistant. Synonyms:OPM1 Cellosaurus code:
Control MM1.S
Human primary cancer cell line derived from the peripheral blood of a patient with multiple myeloma. Dexamethasone-sensitive. Parental cell line:: MM.1 Synonyms:MM.1S; MM1-S; MM-1S; MM1S Cellosaurus code:

IL-1b; IFN-g study 1 (60hrs) / untreated pancreatic islet sample (60hrs)

Relative Expression (log2-ratio):1.6085186
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (60hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 60 hours. Media were changed after 2 days.
Control untreated pancreatic islet sample (60hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 60 hours. Media were changed after 2 days.

glioma study 16 (astrocytoma) / normal brain tissue

Relative Expression (log2-ratio):1.6004267
Number of Samples:8 / 2
Experimental glioma study 16 (astrocytoma)
Primary astrocytoma tissues obtained from the operating room. Tumors were diagnosed and graded according to the current WHO classification (grade III-IV).
Control normal brain tissue
Normal brain tissue sample from healthy donors.

glioma study 16 (secondary glioblastoma) / normal brain tissue

Relative Expression (log2-ratio):1.57549
Number of Samples:3 / 2
Experimental glioma study 16 (secondary glioblastoma)
Secondary glioma tissues obtained from the operating room. Tumors were diagnosed and graded according to the current WHO classification (grade IV).
Control normal brain tissue
Normal brain tissue sample from healthy donors.