TOP TEN perturbations for 39680_at (Homo sapiens)
Organism: Homo sapiens
Gene: 39680_at
Selected probe(set): 206835_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 39680_at (206835_at) across 6674 perturbations tested by GENEVESTIGATOR:
bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)
Relative Expression (log2-ratio):9.990217Number of Samples:3 / 3
| Experimental | bronchial epithelial cell differentiation study 1 (day28) |
| Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour. | |
| Control | bronchial epithelial cell differentiation study 1 (subconfluent) |
| Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert. |
bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)
Relative Expression (log2-ratio):9.6506Number of Samples:3 / 3
| Experimental | bronchial epithelial cell differentiation study 1 (day28) |
| Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour. | |
| Control | bronchial epithelial cell differentiation study 1 (confluent) |
| Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert. |
heat shock study 1 (LPS) / untreated THP-1 cell sample
Relative Expression (log2-ratio):7.1436024Number of Samples:3 / 3
| Experimental | heat shock study 1 (LPS) |
| Cultured THP-1 mononuclear cells were treated with heat shock (43°C; 1h) and lipopolysaccharide (1ug/ml; 4h). | |
| Control | untreated THP-1 cell sample |
| Cultured THP-1 mononuclear cells were cultured in basal growth media at 37°C. |
mucociliary differentiation study 1 (late) / ALI-cultured bronchial epithelial cell sample
Relative Expression (log2-ratio):6.465328Number of Samples:8 / 3
| Experimental | mucociliary differentiation study 1 (late) |
| Primary human bronchial epithelial cells (HBECs) harvested at day 17, 21 and 28 of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells). | |
| Control | ALI-cultured bronchial epithelial cell sample |
| Primary human bronchial epithelial cells (HBECs) harvested at day 0, the first day of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells). |
mucociliary differentiation study 1 (intermediate) / ALI-cultured bronchial epithelial cell sample
Relative Expression (log2-ratio):5.8059683Number of Samples:11 / 3
| Experimental | mucociliary differentiation study 1 (intermediate) |
| Primary human bronchial epithelial cells (HBECs) harvested at day 8, 10, 12 and 14 of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells). | |
| Control | ALI-cultured bronchial epithelial cell sample |
| Primary human bronchial epithelial cells (HBECs) harvested at day 0, the first day of air-liquid interface culture (When cultured at an air-liquid interface, HBECs form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells). |
chronic rhinosinusitis study 1 / normal nasal cavity epithelium sample
Relative Expression (log2-ratio):5.4479914Number of Samples:9 / 9
| Experimental | chronic rhinosinusitis study 1 |
| Nasal cavity epithelium from patients with chronic rhinosinusitis. | |
| Control | normal nasal cavity epithelium sample |
| Normal nasal cavity epithelium. |
trachoma study 1 (C. trachomatis positive) / normal tarsal conjunctiva tissue
Relative Expression (log2-ratio):-4.33095Number of Samples:18 / 20
| Experimental | trachoma study 1 (C. trachomatis positive) |
| Swabs from the tarsal conjunctiva of patients with clinical signs of acute trachoma and infection with Chlamydia trachomatis. | |
| Control | normal tarsal conjunctiva tissue |
| Swabs from the tarsal conjunctiva of subjects without clinical signs of active trachoma in both eyes and absence of C. trachomatis infection. |
sarcoidosis study 6 (lacrimal gland tissue) / normal lacrimal gland tissue
Relative Expression (log2-ratio):-4.114544Number of Samples:12 / 7
| Experimental | sarcoidosis study 6 (lacrimal gland tissue) |
| Lacrimal gland tissue samples obtained from patients who suffered from sarcoidosis. Biopsies were formalin-fixed and paraffin-embedded. | |
| Control | normal lacrimal gland tissue |
| Normal lacrimal gland tissue samples. The control tissue was obtained during surgery on eyes with non-inflamed orbits, such as blepharoplasties and enucleations. Biopsies were formalin-fixed and paraffin-embedded. |
nonspecific orbital inflammatory disease study 1 (lacrimal gland tissue) / normal lacrimal gland tissue
Relative Expression (log2-ratio):-3.6748238Number of Samples:42 / 7
| Experimental | nonspecific orbital inflammatory disease study 1 (lacrimal gland tissue) |
| Lacrimal gland tissue samples obtained from patients who suffered from nonspecific orbital inflammatory disease (NSOI). Biopsies were formalin-fixed and paraffin-embedded. | |
| Control | normal lacrimal gland tissue |
| Normal lacrimal gland tissue samples. The control tissue was obtained during surgery on eyes with non-inflamed orbits, such as blepharoplasties and enucleations. Biopsies were formalin-fixed and paraffin-embedded. |
chronic rhinosinusitis; cystic fibrosis study 1 / normal nasal cavity epithelium sample
Relative Expression (log2-ratio):3.5331125Number of Samples:5 / 9
| Experimental | chronic rhinosinusitis; cystic fibrosis study 1 |
| Nasal cavity epithelium from Cystic Fibrosis patients with chronic rhinosinusitis. | |
| Control | normal nasal cavity epithelium sample |
| Normal nasal cavity epithelium. |