TOP TEN perturbations for 39775_at (Homo sapiens)
Organism: Homo sapiens
Gene: 39775_at
Selected probe(set): 200986_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array
Expression of 39775_at (200986_at) across 6674 perturbations tested by GENEVESTIGATOR:
dendritic cell study 7 (IL-4 mddc) / dendritic cell study 7 (IL-15 mddc)
Relative Expression (log2-ratio):-5.0454645Number of Samples:3 / 3
| Experimental | dendritic cell study 7 (IL-4 mddc) |
| Mature IL-4 dendritic cells differentiated from CD14+ monocytes obtained from healthy donors. Monocytes were seeded in RPMI and supplemented with 2.5 % heat-inactivated human AB serum. Monocytes were cultured with 800 IU/mL granulocyte macrophage colony-stimulating factor and 20 ng/mL IL-4 in order to generate immature IL-4 DCs. Conventional maturation cocktail, comprising 10 ng/mL tumor necrosis factor-α, 10 ng/mL interleukin-1β, 15 ng/ml interleukin-6 and 1 µg/mL prostaglandin E2 was added for 48 hours. CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) by Ficoll density gradient centrifugation and purified by positive magnetic cell selection. | |
| Control | dendritic cell study 7 (IL-15 mddc) |
| Mature IL-15 dendritic cells differentiated from CD14+ monocytes obtained from healthy donors. Monocytes were seeded in RPMI and supplemented with 2.5 % heat-inactivated human AB serum. Differentiation was induced with 800 IU/mL granulocyte macrophage colony-stimulating factor and 200 ng/mL IL-15 to obtain immature IL-15 DCs. A TLR-activating maturation cocktail, comprising 3 µg/mL R848, 2.5 ng/mL tumor necrosis factor-α, 250 ng/mL interferon-γ and 1 µg/mL prostaglandin E2 was added after 24-48 hours of differentiation for 18-20 hours. CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) by Ficoll density gradient centrifugation and purified by positive magnetic cell selection. |
HIVGFP(G); SIVVLP(G) study 1 / uninfected monocyte-derived dendritic cell sample
Relative Expression (log2-ratio):4.977375Number of Samples:2 / 2
| Experimental | HIVGFP(G); SIVVLP(G) study 1 |
| Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with GFP-encoding HIV-1 pseudotyped with VSV-G [HIVGFP(G)] and VSV-G-pseudotyped SIVmac239 virus-like particles [SIVVLP(G)] for 48 hours. | |
| Control | uninfected monocyte-derived dendritic cell sample |
| Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were treated with lipopolysaccharide (LPS) for 48 hours. |
IFN-g study 9 (24h) / untreated epidermal keratinocyte sample
Relative Expression (log2-ratio):4.960952Number of Samples:3 / 6
| Experimental | IFN-g study 9 (24h) |
| Primary keratinocytes treated with interferon gamma (IFN-g) for 24 hours. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency. Cultures were starved of growth factors in nonsupplemented M154 medium for 24 hours, and afterwards stimulated with recombinant human IFN-g for 24 hours. | |
| Control | untreated epidermal keratinocyte sample |
| Untreated primary epidermal keratinocytes. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency. |
hepatocyte (ESC) / HepaRG
Relative Expression (log2-ratio):-4.7128353Number of Samples:8 / 12
| Experimental | hepatocyte (ESC) |
| Hepatocyte-like cells differentiated from embryonic stem cells (ESC) | |
| Control | HepaRG |
| Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code: |
HIVGFP(G); SIVVLP(G) study 1 / HIVGFP(G) study 1
Relative Expression (log2-ratio):4.667369Number of Samples:2 / 2
| Experimental | HIVGFP(G); SIVVLP(G) study 1 |
| Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with GFP-encoding HIV-1 pseudotyped with VSV-G [HIVGFP(G)] and VSV-G-pseudotyped SIVmac239 virus-like particles [SIVVLP(G)] for 48 hours. | |
| Control | HIVGFP(G) study 1 |
| Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with GFP-encoding HIV-1 pseudotyped with VSV-G [HIVGFP(G)] for 48 hours. |
HIVGFP(G); SIVVLP(G) study 1 / SIVVLP(G) study 1
Relative Expression (log2-ratio):4.6412964Number of Samples:2 / 2
| Experimental | HIVGFP(G); SIVVLP(G) study 1 |
| Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with GFP-encoding HIV-1 pseudotyped with VSV-G [HIVGFP(G)] and VSV-G-pseudotyped SIVmac239 virus-like particles [SIVVLP(G)] for 48 hours. | |
| Control | SIVVLP(G) study 1 |
| Monocyte-derived dendritic cell samples (MDDC) from isolated and stimulated CD14+ monocytes (GM-CSF and IL-4 for dendritic cell differentiation) of adult peripheral blood. MDDC were infected with VSV-G-pseudotyped SIVmac239 virus-like particles [SIVVLP(G)] for 48 hours. |
Hep-G2 / HepaRG
Relative Expression (log2-ratio):-4.614258Number of Samples:9 / 12
| Experimental | Hep-G2 |
| Human primary cancer cell line derived from the liver of a patient with hepatocellular carcinoma. Synonyms:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 Cellosaurus code: | |
| Control | HepaRG |
| Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code: |
Dengue hemorrhagic fever study 1 (2d) / Dengue hemorrhagic fever study 1 (convalescent)
Relative Expression (log2-ratio):4.548005Number of Samples:3 / 12
| Experimental | Dengue hemorrhagic fever study 1 (2d) |
| Peripheral blood mononuclear cell (PBMC) samples derived from the blood of Venezuelan individuals 2 days after the onset of Dengue hemorrhagic fever. | |
| Control | Dengue hemorrhagic fever study 1 (convalescent) |
| Peripheral blood mononuclear cell (PBMC) samples derived from the blood of Venezuelan individuals 28 days after the onset of Dengue hemorrhagic fever. |
Dengue fever study 1 (early) / Dengue fever study 1 (convalescent)
Relative Expression (log2-ratio):4.483945Number of Samples:2 / 11
| Experimental | Dengue fever study 1 (early) |
| Peripheral blood mononuclear cell (PBMC) samples derived from the blood of Venezuelan individuals one day after the onset of Dengue fever. | |
| Control | Dengue fever study 1 (convalescent) |
| Peripheral blood mononuclear cell (PBMC) samples derived from the blood of Venezuelan individuals 28 days after the onset of Dengue fever. |
prostate cancer study 8 (p. canc) / prostate cancer study 8 (ptasc)
Relative Expression (log2-ratio):-4.4684486Number of Samples:3 / 2
| Experimental | prostate cancer study 8 (p. canc) |
| CD26+ FACS sorted prostate neoplasm cell (p. canc) samples from patients with primary prostate cancer collected after radical prostatectomy. | |
| Control | prostate cancer study 8 (ptasc) |
| CD90+ FACS sorted prostate tumor-associated stromal cell (ptasc) samples from patients with primary prostate cancer collected after radical prostatectomy. |