TOP TEN perturbations for 39827_at (Homo sapiens)

Organism: Homo sapiens
Gene: 39827_at
Selected probe(set): 202887_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39827_at (202887_s_at) across 6674 perturbations tested by GENEVESTIGATOR:

actinomycin D study 1 / mannitol treated A549 cell sample

Relative Expression (log2-ratio):-6.9599714
Number of Samples:3 / 3
Experimental actinomycin D study 1
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. ATC code:
Control mannitol treated A549 cell sample
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture.

E. coli study 2 / unstimulated, normal monocyte-derived macrophage sample

Relative Expression (log2-ratio):5.0487814
Number of Samples:5 / 7
Experimental E. coli study 2
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours with 2.5 × 105 heat-killed E. coli.
Control unstimulated, normal monocyte-derived macrophage sample
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours unstimulated.

Langerhans cell histiocytosis study 1 / normal myeloid dendritic cell sample

Relative Expression (log2-ratio):-4.7940445
Number of Samples:7 / 3
Experimental Langerhans cell histiocytosis study 1
Langerhans cell histiocytes (LCH) were isolated from LCH lesions of patients undergoing surgery. All patients had single system disease, five patients had bone lesion, one had skin lesion and one had mucosal manifestation. Langerhans cells histiocytes were purified by FACS as CD1a+ / CD270 + population with > 95% purity.
Control normal myeloid dendritic cell sample
Normal myeloid dendritic cells were isolated from peripheral blood of healthy adult donors. Myeloid dendritic cells were defined as HLA-DR+ / CD11c + / BDCA1+ / BDCA3- population.

RAF1 depletion study 2 / control vector transfected SW-620 cell sample

Relative Expression (log2-ratio):-4.7937136
Number of Samples:6 / 6
Experimental RAF1 depletion study 2
RNAi-mediated gene knockdown of RAF1 in colorectal cancer cell line SW-620.
Control control vector transfected SW-620 cell sample
SW-620 colorectal cell line sample transduced with empty vector pLKO.

Langerhans cell histiocytosis study 1 / normal plasmocytoid dendritic cell sample

Relative Expression (log2-ratio):-4.7288084
Number of Samples:7 / 3
Experimental Langerhans cell histiocytosis study 1
Langerhans cell histiocytes (LCH) were isolated from LCH lesions of patients undergoing surgery. All patients had single system disease, five patients had bone lesion, one had skin lesion and one had mucosal manifestation. Langerhans cells histiocytes were purified by FACS as CD1a+ / CD270 + population with > 95% purity.
Control normal plasmocytoid dendritic cell sample
Normal plasmocytoid dendritic cells were isolated from peripheral blood of healthy adult donors. Plasmocytoid dendritic cells were defined as HLA-DR+ / CD45RAhi / CD11c- / BDCA2+ population.

DHA study 1 (6h) / vehicle (ethanol) treated SW620 cell sample (6h)

Relative Expression (log2-ratio):4.6551332
Number of Samples:3 / 3
Experimental DHA study 1 (6h)
The human colon adenocarcinoma SW620 cells treated with docosahexaenoic acid (DHA, 70uM) for 6 hours. Cells were cultured in Leibowitz’s L-15 medium supplemented with L-glutamine (2 mM), FBS (10%) and gentamicin (45 mg/l) and maintained in a humidified atmosphere of 5% CO2: 95% air at 37°C. DHA stock solution was dissolved in ethanol. Final concentration of ethanol in the medium was less than 0.01% v/v.
Control vehicle (ethanol) treated SW620 cell sample (6h)
The human colon adenocarcinoma SW620 cells treated with vehicle (ethanol) for 6 hours. Cells were cultured in Leibowitz’s L-15 medium supplemented with L-glutamine (2 mM), FBS (10%) and gentamicin (45 mg/l) and maintained in a humidified atmosphere of 5% CO2: 95% air at 37°C. Final concentration of ethanol in the medium was less than 0.01% v/v.

echinomycin study 1 / deferoxamine study 5

Relative Expression (log2-ratio):-4.3426247
Number of Samples:3 / 3
Experimental echinomycin study 1
Echinomycin (100nM; 2h) treated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:---
Control deferoxamine study 5
Untreated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:

tumor supernatant activation study 2 / untreated memory CD4 T-cell sample

Relative Expression (log2-ratio):4.3249903
Number of Samples:4 / 3
Experimental tumor supernatant activation study 2
Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium for 24 hours before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g.
Control untreated memory CD4 T-cell sample
Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

RAF1 depletion study 2 / mock transfected SW-620 cell sample

Relative Expression (log2-ratio):-4.3117476
Number of Samples:6 / 6
Experimental RAF1 depletion study 2
RNAi-mediated gene knockdown of RAF1 in colorectal cancer cell line SW-620.
Control mock transfected SW-620 cell sample
SW-620 colorectal cell line sample transduced with non-targeting pLKO_NonTarget vector.

NGFR depletion study 2 / control vector transfected SW-620 cell sample

Relative Expression (log2-ratio):-4.279293
Number of Samples:6 / 6
Experimental NGFR depletion study 2
RNAi-mediated gene knockdown of NGFR in colorectal cancer cell line SW-620.
Control control vector transfected SW-620 cell sample
SW-620 colorectal cell line sample transduced with empty vector pLKO.