Organism: Homo sapiens
Gene: 39935_at
Selected probe(set): 225227_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39935_at (225227_at) across 6674 perturbations tested by GENEVESTIGATOR:

Relative Expression (log2-ratio):5.1443577
Number of Samples:4 / 3

Experimental	tumor supernatant activation study 3
	Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated for 24 hours in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium supplemented with antiCD3/CD28 beads, before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g. ATC code:---
Control	untreated memory CD4 T-cell sample
	Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

Relative Expression (log2-ratio):4.8556557
Number of Samples:3 / 3

Experimental	tumor supernatant activation study 1
	Total CD4+ T cells isolated from peripheral blood of female healthy donors were incubated in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium for 24 hours before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g.
Control	untreated CD4 T-cell sample
	Total CD4+ T cells were isolated from peripheral blood of female healthy donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

Relative Expression (log2-ratio):4.6310987
Number of Samples:4 / 3

Experimental	tumor supernatant activation study 2
	Memory (CD45RA-) CD4+ T cells isolated from peripheral blood of female healthy donors were incubated in 1:1 mix of tumor supernatant from primary invasive breast ductal carcinoma and X-VIVO 20 medium for 24 hours before harvest for RNA isolation. The tumor supernatant was prepared as followed: fresh surgical specimen was dissociated in X-VIVO 20 medium by GentleMACS dissociator and the resulting suspension was clarified by centrifugation for 15min at 13'000 g.
Control	untreated memory CD4 T-cell sample
	Memory (CD45RA-) CD4+ T-cells were isolated from peripheral blood of healthy female donors and incubated in X-VIVO 20 medium for 24 hours, before harvest for RNA isolation.

Relative Expression (log2-ratio):4.5163803
Number of Samples:2 / 2

Experimental	LPS study 4 (shRNA cycT1)
	MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control	cycT1 depletion study 2 (shRNA)
	MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then mock treated.

Relative Expression (log2-ratio):4.516178
Number of Samples:2 / 2

Experimental	LPS study 4
	MONO-MAC-6 (MM6) cells were treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control	mock treated MONO-MAC-6 cell sample
	MONO-MAC-6 (MM6) cells mock treated.

Relative Expression (log2-ratio):-4.494212
Number of Samples:3 / 3

Experimental	echinomycin study 1
	Echinomycin (100nM; 2h) treated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:---
Control	deferoxamine study 5
	Untreated human astroglioma (U251) cells, stimulated with deferoxamine (DFO; 300mM; 16h). ATC code:

Relative Expression (log2-ratio):4.164671
Number of Samples:2 / 2

Experimental	LPS study 4 (shRNA contr.)
	MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control	mock treated / transduced MONO-MAC-6 cell sample
	MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then mock treated.

Relative Expression (log2-ratio):3.910531
Number of Samples:2 / 3

Experimental	brefeldin A study 1 (0.5ug/ml; p53HCT116)
	Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control	untreated p53HCT116 cell sample
	Derived human colon carcinoma cell line p53HCT116 with knockout gene for p53 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

Relative Expression (log2-ratio):3.777565
Number of Samples:8 / 8

Experimental	PMA; ionomycine study 2
	CD4+ memory T cells derived from peripheral blood of healthy subjects were stimulated for 3 hours with phorbol 12-myristate 13-acetate (PMA, 10 ng/ml) and ionomycin (1 µg/ml). ATC code:---
Control	unstimulated CD4 memory T-cell sample
	Unstimulated CD4+ memory T cells derived from peripheral blood of healthy subjects.

Relative Expression (log2-ratio):3.5159254
Number of Samples:3 / 3

Experimental	brefeldin A study 1 (0.5ug/ml; HCT 116)
	Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control	untreated HCT 116 cell sample
	Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.