TOP TEN perturbations for 39988_at (Homo sapiens)

Organism: Homo sapiens
Gene: 39988_at
Selected probe(set): 225973_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 39988_at (225973_at) across 6674 perturbations tested by GENEVESTIGATOR:

IL-1b; IFN-g study 1 (24hrs) / untreated pancreatic islet sample (24hrs)

Relative Expression (log2-ratio):4.2622814
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (24hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 24 hours.
Control untreated pancreatic islet sample (24hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 24 hours.

IL-1b; IFN-g study 1 (84hrs) / untreated pancreatic islet sample (84hrs)

Relative Expression (log2-ratio):4.0085144
Number of Samples:3 / 3
Experimental IL-1b; IFN-g study 1 (84hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 84 hours. Media were changed after 2 days.
Control untreated pancreatic islet sample (84hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 84 hours. Media were changed after 2 days.

C. pneumoniae study 1 / uninfected monocyte-derived dendritic cell sample

Relative Expression (log2-ratio):3.7547665
Number of Samples:2 / 2
Experimental C. pneumoniae study 1
Monocyte-derived dendritic cells from healthy donors were infected with Chlamydia pneumoniae at a multiplicity of infection of 5 for 72 h.
Control uninfected monocyte-derived dendritic cell sample
Uninfected monocyte-derived dendritic cells from healthy donors.

IL-1b; IFN-g study 1 (132hrs) / untreated pancreatic islet sample (132hrs)

Relative Expression (log2-ratio):3.7002983
Number of Samples:2 / 2
Experimental IL-1b; IFN-g study 1 (132hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 132 hours. Media were changed after 2 and 5 days.
Control untreated pancreatic islet sample (132hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 132 hours. Media were changed after 2 and 5 days.

expO breast cancer study 1 (papillary carcinoma, NOS; primary) / expO breast cancer study 1 (medullary carcinoma, NOS; primary)

Relative Expression (log2-ratio):-3.689104
Number of Samples:2 / 2
Experimental expO breast cancer study 1 (papillary carcinoma, NOS; primary)
Primary tumor tissue samples obtained from the breast of patients with papillary carcinoma (NOS).
Control expO breast cancer study 1 (medullary carcinoma, NOS; primary)
Primary tumor tissue samples obtained from the breast of patients with medullary carcinoma (NOS).

expO breast cancer study 1 (papillary carcinoma, NOS; primary) / expO breast cancer study 1 (adenocarcinoma with squamous metaplasia; primary)

Relative Expression (log2-ratio):-3.6536665
Number of Samples:2 / 2
Experimental expO breast cancer study 1 (papillary carcinoma, NOS; primary)
Primary tumor tissue samples obtained from the breast of patients with papillary carcinoma (NOS).
Control expO breast cancer study 1 (adenocarcinoma with squamous metaplasia; primary)
Primary tumor tissue samples obtained from the breast of patients with adenocarcinoma with squamous metaplasia.

T. cruzi study 1 (BJ; 24h; bottom) / mock infected foreskin fibroblast (BJ) sample (24h; bottom)

Relative Expression (log2-ratio):3.5801382
Number of Samples:2 / 2
Experimental T. cruzi study 1 (BJ; 24h; bottom)
Foreskin fibroblast (BJ) cells grown in the transwell bottom, infected with Trypanosoma cruzi trypomastigotes for 2 hours (10exp8/ml) and harvested 24 hours post infection. Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin.
Control mock infected foreskin fibroblast (BJ) sample (24h; bottom)
Foreskin fibroblast (BJ) cells grown in the transwell bottom, mock-infected and harvested 24 hours post mock infection with 2% FBS medium. Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin.

glioma study 17 (glioblastoma; unsorted) / non-tumor cortical tissue

Relative Expression (log2-ratio):3.5712566
Number of Samples:2 / 4
Experimental glioma study 17 (glioblastoma; unsorted)
Brain cells isolated from high grade glioblastoma (grade IV). Tumor tissue was dissociated using enzymatic treatment and all cells were used for RNA isolation.
Control non-tumor cortical tissue
Cortical tissue obtained from patients with epilepsy, but without any manifested brain cancer. The tissue was dissociated using enzymatic treatment, but all brain cell types were used for analyses.

TNF-ɑ; TGF-ß2 study 1 (intermediate) / untreated ARPE-19 cell sample

Relative Expression (log2-ratio):3.5144234
Number of Samples:6 / 3
Experimental TNF-ɑ; TGF-ß2 study 1 (intermediate)
ARPE-19 retina pigment epithelial cell samples treated with TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml). Samples were taken 16 and 24 hours after treatment.
Control untreated ARPE-19 cell sample
Untreated ARPE-19 retina pigment epithelial cell samples harvested before adding TNF-ɑ (10 ng/ml) and TGF-ß2 (5 ng/ml).

stem cell differentiation study 59 (iDRG; 15d) / stem cell differentiation study 59 (8d)

Relative Expression (log2-ratio):3.5127335
Number of Samples:4 / 4
Experimental stem cell differentiation study 59 (iDRG; 15d)
Immature dorsal root ganglia neurons (iDRGs) obtained by differentiation of WA09 embryonic stem cells. WA09 cells were differentiated for 8 days and subsequently cryopreserved. After thawing, cells were further differentiated for 7 days. Further details are described in the paper.
Control stem cell differentiation study 59 (8d)
WA09 embryonic stem cell samples differentiated for 8 days. Further details are described in the paper.