TOP TEN perturbations for 48031_r_at (Homo sapiens)

Organism: Homo sapiens
Gene: 48031_r_at
Selected probe(set): 48030_i_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 48031_r_at (48030_i_at) across 6672 perturbations tested by GENEVESTIGATOR:

BEZ235 study 1 (3.606 uM) / vehicle (DMSO) treated A2058 cell samples

Relative Expression (log2-ratio):2.7350235
Number of Samples:4 / 4
Experimental BEZ235 study 1 (3.606 uM)
A2058 cells treated with 3.606 uM NVP-BEZ235 for 24hours. The concentration corresponding to the IC90 of BKM120. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine. BEZ235 is a mTOR/PI3K inhibitor; alternative names: [NVP-BEZ235] ATC code:---
Control vehicle (DMSO) treated A2058 cell samples
A2058 cells treated with vehicle (0.1% DMSO) for 24hours. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine.

BEZ235 study 1 (IC90) / vehicle (DMSO) treated A2058 cell samples

Relative Expression (log2-ratio):2.5584822
Number of Samples:4 / 4
Experimental BEZ235 study 1 (IC90)
A2058 cells treated with NVP-BEZ235 for 24hours at an IC90 concentration. Concentration of IC90 was judged by reduction of pAkt levels (S473P-Akt inhibition) and the value is not clear from the article. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine. BEZ235 is a mTOR/PI3K inhibitor; alternative names: [NVP-BEZ235] ATC code:---
Control vehicle (DMSO) treated A2058 cell samples
A2058 cells treated with vehicle (0.1% DMSO) for 24hours. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine.

systemic onset JIA study 4 / oligoarticular JIA study 3

Relative Expression (log2-ratio):1.9467211
Number of Samples:3 / 12
Experimental systemic onset JIA study 4
Peripheral blood mononuclear cell (PBMC) samples from children with systemic onset of juvenile rheumatoid arthritis (JIA).
Control oligoarticular JIA study 3
Peripheral blood mononuclear cell (PBMC) samples from children with persistent oligoarthritis (oligoarticular JIA).

systemic onset JIA study 5 / oligoarticular JIA study 2

Relative Expression (log2-ratio):1.8953838
Number of Samples:3 / 12
Experimental systemic onset JIA study 5
Peripheral blood mononuclear cell (PBMC) samples from children with recent onset and systemic onset of juvenile rheumatoid arthritis (SoJIA). All patients were free of disease modifying antirheumatic drugs or biologics. Subjects were classified according to ILAR criteria. Patients were enrolled in the study very early in the disease course, hence difference between disease onset and age at sampling is small (<4-8 month).
Control oligoarticular JIA study 2
Peripheral blood mononuclear cell (PBMC) samples from children with recent onset of persistent oligoarticular juvenile idiopathic arthritis (JIA). All patients were free of disease modifying antirheumatic drugs or biologics. Subjects were classified according to ILAR criteria. Patients were enrolled in the study very early in the disease course, hence difference between disease onset and age at sampling is small (<4-8 month).

BEZ235 study 1 (IC50) / vehicle (DMSO) treated A2058 cell samples

Relative Expression (log2-ratio):1.8372889
Number of Samples:4 / 4
Experimental BEZ235 study 1 (IC50)
A2058 cells treated with NVP-BEZ235 for 24hours at an IC50 concentration. Concentration of IC50 was judged by reduction of pAkt levels (S473P-Akt inhibition) and the value is not clear from the article. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine. BEZ235 is a mTOR/PI3K inhibitor; alternative names: [NVP-BEZ235] ATC code:---
Control vehicle (DMSO) treated A2058 cell samples
A2058 cells treated with vehicle (0.1% DMSO) for 24hours. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine.

sarcoidosis study 6 (orbital adipose tissue) / normal orbital adipose tissue

Relative Expression (log2-ratio):-1.8293104
Number of Samples:7 / 22
Experimental sarcoidosis study 6 (orbital adipose tissue)
Orbital adipose tissue samples obtained from patients who suffered from sarcoidosis. Biopsies were formalin-fixed and paraffin-embedded.
Control normal orbital adipose tissue
Normal orbital adipose tissue samples. Samples were obtained from subjects with no history of orbital disease at the time of surgery such as fat typically excised and discarded as part of routine blepharoplasty or retrobulbar fat that is snared with the eye during enucleation. Biopsies were formalin-fixed and paraffin-embedded.

GDC-0941 study 1 (3.606 uM) / vehicle (DMSO) treated A2058 cell samples

Relative Expression (log2-ratio):1.8178921
Number of Samples:4 / 4
Experimental GDC-0941 study 1 (3.606 uM)
A2058 cells treated with 3.606 uM GDC-0941 for 24hours. The concentration corresponding to the IC90 of BKM120. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine. GDC-0941 is a potent inhibitor of PI3Kα/δ, alternative names: [pictilisib] ATC code:---
Control vehicle (DMSO) treated A2058 cell samples
A2058 cells treated with vehicle (0.1% DMSO) for 24hours. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine.

BEZ235 study 1 (3.606 uM) / BKM120 study 1 (IC90; 3.606 uM)

Relative Expression (log2-ratio):1.7599459
Number of Samples:4 / 4
Experimental BEZ235 study 1 (3.606 uM)
A2058 cells treated with 3.606 uM NVP-BEZ235 for 24hours. The concentration corresponding to the IC90 of BKM120. Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine. BEZ235 is a mTOR/PI3K inhibitor; alternative names: [NVP-BEZ235] ATC code:---
Control BKM120 study 1 (IC90; 3.606 uM)
A2058 cells treated with BKM120 for 24hours at an IC90 concentration of 3.606 uM. Concentration of IC90 was judged by reduction of pAkt levels (S473P-Akt inhibition). Cells were maintained in DMEM, high glucose, 10% FCS, 1% pyruvate, 2% glutamine. BKM120 is a pan-PI3K inhibitor; alternative names: [buparlisib, NVP-BKM120] ATC code:---

glioma study 17 (anaplastic astrocytoma; A2B5+) / non-tumor oligodendrocyte progenitor cell sample (cortex)

Relative Expression (log2-ratio):-1.7220621
Number of Samples:2 / 3
Experimental glioma study 17 (anaplastic astrocytoma; A2B5+)
Oligodendrocyte progenitor cells (OPC) isolated from high grade anaplastic astrocytoma (grade III). OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen. Patients were 45 ± 18 years old.
Control non-tumor oligodendrocyte progenitor cell sample (cortex)
Oligodendrocyte progenitor cells (OPC) isolated from cortical tissue, which was obtained from patients with epilepsy, but without any manifested brain cancer. OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen.

systemic onset JIA study 4 / normal PBMC sample

Relative Expression (log2-ratio):1.7113495
Number of Samples:3 / 27
Experimental systemic onset JIA study 4
Peripheral blood mononuclear cell (PBMC) samples from children with systemic onset of juvenile rheumatoid arthritis (JIA).
Control normal PBMC sample
Peripheral blood mononuclear cell (PBMC) samples from healthy children.