Organism: Homo sapiens
Gene: METTL15
Selected probe(set): 238773_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of METTL15 (238773_at) across 6540 perturbations tested by GENEVESTIGATOR:

Relative Expression (log2-ratio):-2.404354
Number of Samples:3 / 3

Experimental	formaldehyde study 3 (4500ug/ml)
	Bronchial epithelial cells (NHBE) treated with 4500 ug/ml formaldehyde (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 24 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:---
Control	vehicle (EtOH) treated bronchial epithelial cell sample
	Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 24 hours. NHBE cells were derived from a 60 year old male non-smoker.

Relative Expression (log2-ratio):2.2836523
Number of Samples:3 / 3

Experimental	influenza virus study 10 (A/H5N2)
	Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F189/07/2004(H5N2). Samples were taken 10 hours post-infection.
Control	influenza virus study 4 (A/H1N1)
	Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

Relative Expression (log2-ratio):-2.1514606
Number of Samples:2 / 2

Experimental	T-cell isolation study 11
	CD4+ resting memory T-cell were isolated from peripheral blood buffy coat of healthy donors after storage for 24 hours at 20°C. The cell fraction was first enriched via density gradient centrifugation with LSM 1077 Ficoll and than FACS sorted as CD3+/CD4+/CD45RO+/CD45RA- population.
Control	T-cell isolation study 6
	CD4+ resting memory T-cell were isolated from whole peripheral blood of healthy donors with no delay. The cell fraction was first enriched via density gradient centrifugation with LSM 1077 Ficoll and than FACS sorted as CD3+/CD4+/CD45RO+/CD45RA- population.

Relative Expression (log2-ratio):-2.1220617
Number of Samples:2 / 2

Experimental	T-helper activation study 1 (160min)
	Alloantigen specific helper T-cells were stimulated for 160min with anti-CD3/anti-CD28/IL-2 (100U/ml). T-helper cells were sorted as CD4+ CD25- cells from peripheral blood of healthy donors.
Control	unstimulated helper T-cell sample
	Unstimulated alloantigen specific helper T-cell sample derived from sorted CD4+ CD25- cells from peripheral blood of healthy donors.

Relative Expression (log2-ratio):2.0998955
Number of Samples:3 / 3

Experimental	influenza virus study 11 (A/H5N3)
	Human carcinoma cell line A549 infected with influenza A virus subtype influenza virus A/duck/Malaysia/F119/3/1997(H5N3). Samples were taken 10 hours post-infection.
Control	influenza virus study 4 (A/H1N1)
	Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

Relative Expression (log2-ratio):-2.0779743
Number of Samples:8 / 8

Experimental	PMA; ionomycine study 2
	CD4+ memory T cells derived from peripheral blood of healthy subjects were stimulated for 3 hours with phorbol 12-myristate 13-acetate (PMA, 10 ng/ml) and ionomycin (1 µg/ml). ATC code:---
Control	unstimulated CD4 memory T-cell sample
	Unstimulated CD4+ memory T cells derived from peripheral blood of healthy subjects.

Relative Expression (log2-ratio):-1.9539604
Number of Samples:2 / 2

Experimental	T-helper activation study 1 (180min)
	Alloantigen specific helper T-cells were stimulated for 180min with anti-CD3/anti-CD28/IL-2 (100U/ml). T-helper cells were sorted as CD4+ CD25- cells from peripheral blood of healthy donors.
Control	unstimulated helper T-cell sample
	Unstimulated alloantigen specific helper T-cell sample derived from sorted CD4+ CD25- cells from peripheral blood of healthy donors.

Relative Expression (log2-ratio):-1.9522076
Number of Samples:2 / 2

Experimental	T-helper activation study 1 (240min)
	Alloantigen specific helper T-cells were stimulated for 240min with anti-CD3/anti-CD28/IL-2 (100U/ml). T-helper cells were sorted as CD4+ CD25- cells from peripheral blood of healthy donors.
Control	unstimulated helper T-cell sample
	Unstimulated alloantigen specific helper T-cell sample derived from sorted CD4+ CD25- cells from peripheral blood of healthy donors.

Relative Expression (log2-ratio):-1.9031057
Number of Samples:2 / 2

Experimental	T-helper activation study 1 (200min)
	Alloantigen specific helper T-cells were stimulated for 200min with anti-CD3/anti-CD28/IL-2 (100U/ml). T-helper cells were sorted as CD4+ CD25- cells from peripheral blood of healthy donors.
Control	unstimulated helper T-cell sample
	Unstimulated alloantigen specific helper T-cell sample derived from sorted CD4+ CD25- cells from peripheral blood of healthy donors.

Relative Expression (log2-ratio):-1.856494
Number of Samples:2 / 2

Experimental	T-helper activation study 1 (280min)
	Alloantigen specific helper T-cells were stimulated for 280min with anti-CD3/anti-CD28/IL-2 (100U/ml). T-helper cells were sorted as CD4+ CD25- cells from peripheral blood of healthy donors.
Control	unstimulated helper T-cell sample
	Unstimulated alloantigen specific helper T-cell sample derived from sorted CD4+ CD25- cells from peripheral blood of healthy donors.