TOP TEN perturbations for 4537 (Homo sapiens)

Organism: Homo sapiens
Gene: 4537
Selected probe(set): 1553588_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 4537 (1553588_at) across 5339 perturbations tested by GENEVESTIGATOR:

HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81) / hypoxia study 11 (AB81)

Relative Expression (log2-ratio):2.1345816
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 ?C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 ?C in 6-well plates.
Control hypoxia study 11 (AB81)
Human podocyte cell line AB81 treated with hypoxia (1% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 ?C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 ?C in 6-well plates.

hypoxia study 11 (AB81) / control AB81 cell sample (normoxia)

Relative Expression (log2-ratio):-1.9026499
Number of Samples:3 / 3
Experimental hypoxia study 11 (AB81)
Human podocyte cell line AB81 treated with hypoxia (1% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 ?C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 ?C in 6-well plates.
Control control AB81 cell sample (normoxia)
Human podocyte cell line AB81 treated with normoxia (20% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 ?C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 ?C in 6-well plates.

polycystic ovary syndrome study 6 / normal granulosa cell sample

Relative Expression (log2-ratio):1.7380733
Number of Samples:5 / 2
Experimental polycystic ovary syndrome study 6
Granulosa cell sample derived from women with polycystic ovary syndrome (PCOS) after controlled ovarian hyperstimulation, as a part of standard in vitro fertilization (IVF) protocol. The PCOS was diagnosed according to Rotterdam revised criteria. All subjects were < 35 years with normal prolactin levels and normal thyroid function.
Control normal granulosa cell sample
Granulosa cell sample derived from normal ovulatory women after controlled ovarian hyperstimulation, as a part of standard in vitro fertilization (IVF) protocol. The reason for IVF was male or tubal factor infertility. All subjects were < 35 years with normal prolactin levels, normal thyroid function, regular menstrual cycle, no clinical or biochemical evidence of hyperandrogenism, no polycystic ovaries and normal insulin sensitivity.

HIF-2a depletion study 1 (hypoxia; AB81) / hypoxia study 11 (AB81)

Relative Expression (log2-ratio):1.7309332
Number of Samples:3 / 3
Experimental HIF-2a depletion study 1 (hypoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-2a (Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 ?C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 ?C in 6-well plates.
Control hypoxia study 11 (AB81)
Human podocyte cell line AB81 treated with hypoxia (1% O2) for 24 hours. Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 ?C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 ?C in 6-well plates.

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-1.6359844
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).

plicamycin study 2 (100 nM) / vehicle (PBS) treated TC-71 cell sample

Relative Expression (log2-ratio):-1.6105022
Number of Samples:3 / 3
Experimental plicamycin study 2 (100 nM)
TC-71 Ewing?s sarcoma cells treated with compound: plicamycin (mithramycin, 100 nM) for 6 hours. ATC code:
Control vehicle (PBS) treated TC-71 cell sample
TC-71 Ewing?s sarcoma cells treated with 0.01% phosphate-buffered saline (PBS) in growth medium for 6 hours.

HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81) / HIF-1a depletion study 2 (hypoxia; AB81)

Relative Expression (log2-ratio):1.589776
Number of Samples:3 / 3
Experimental HIF-1a/HIF-2a depletion study 1 (hypoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a and HIF-2a (Hypoxia-inducible factor 1-alpha and Hypoxia-inducible factor 2-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 ?C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 ?C in 6-well plates.
Control HIF-1a depletion study 2 (hypoxia; AB81)
Human podocyte cells AB81 with shRNA-mediated stable knock-down of HIF-1a (Hypoxia-inducible factor 1-alpha) 24 hours after treatment with hypoxia (1% O2). Conditionally immortalized human podocytes AB81 were cultured under permissive conditions (33 ?C) in RPMI-1640 supplemented with 10% FBS, antibiotics, and 1% ITS. Differentiation was induced by thermoshift to 37 ?C in 6-well plates.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):1.4405403
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

atopic dermatitis study 21 (lesional; whole skin) / normal skin tissue

Relative Expression (log2-ratio):-1.4382935
Number of Samples:5 / 6
Experimental atopic dermatitis study 21 (lesional; whole skin)
Lesional full thickness skin samples isolated from patient with moderate-to-severe atopic dermatitis by laser capture microdissection. Patients' cohort characteristics: 3 males and 2 females; age 27-59 years (mean age: 39.4 years); SCORing of Atopic Dermatitis index (SCORAD) ranging from 45-65; total IgE: 14-1821 kU/l; eosinophilic count: 1.4-11.8 %.
Control normal skin tissue
Full thickness skin samples isolated from healthy subjects by laser capture microdissection.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):1.4315643
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.