TOP TEN perturbations for 4539 (Homo sapiens)

Organism: Homo sapiens
Gene: 4539
Selected probe(set): 224373_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of 4539 (224373_s_at) across 5392 perturbations tested by GENEVESTIGATOR:

kidney transplantation study 15 (8 week) / normal monocyte (CD14+) sample

Relative Expression (log2-ratio):-2.011963
Number of Samples:2 / 5
Experimental kidney transplantation study 15 (8 week)
CD14+ monocyte samples derived from kidney transplant patients 8 weeks post-transplantation. Samples were collected 8 week after transplantation and administration of immunosuppressive therapy (day 1-4: methylprednisolone (60 mg); 3 doses: rabbit polyclonal anti-thymocyte globulin (ThymoglobulinH; 6 mg/kg); mycophenolate mofetil (CellCeptH); and tacrolimus (PrografH).
Control normal monocyte (CD14+) sample
CD14+ monocyte samples derived from healthy control subjects.

glioma study 16 (LN-215) / normal astrocyte sample

Relative Expression (log2-ratio):1.8515244
Number of Samples:2 / 3
Experimental glioma study 16 (LN-215)
Human glioma cell line LN215 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (BS-149) / normal astrocyte sample

Relative Expression (log2-ratio):1.8220844
Number of Samples:2 / 3
Experimental glioma study 16 (BS-149)
Human glioma cell line BS149 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):1.758461
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-319) / normal astrocyte sample

Relative Expression (log2-ratio):1.7024212
Number of Samples:2 / 3
Experimental glioma study 16 (LN-319)
Human glioma cell line LN319 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

LRPPRC depletion study 1 (9% resid. exp.) / control shRNA infected MCH58 cell sample

Relative Expression (log2-ratio):-1.6923027
Number of Samples:2 / 2
Experimental LRPPRC depletion study 1 (9% resid. exp.)
MCH58 cells were infected with lentiviral-shRNA resulting in 9% residual expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC). Decreased LRPPRC expression resulted in proportional impairment of mitochondrial OXPHOS (oxidative phosphorylation) system function. Mutated LRPPRC is associated with Leigh syndrome, French-Canadian type (LSFC).
Control control shRNA infected MCH58 cell sample
MCH58 cells were mock infected with off-target lentiviral-shRNA. The cells kept 100% expression of leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC).

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):1.6470451
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

sapphyrin PCI-2050 study 1 / mannitol treated A549 cell sample

Relative Expression (log2-ratio):-1.6016979
Number of Samples:3 / 3
Experimental sapphyrin PCI-2050 study 1
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. ATC code:---
Control mannitol treated A549 cell sample
A549 human lung cancer cells were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture.

ARC study 1 / untreated MCF-7 cell sample

Relative Expression (log2-ratio):-1.5755882
Number of Samples:2 / 2
Experimental ARC study 1
MCF7 cells treated with 2uM ARC (4-amino-6-hydrazino-7-beta-d-ribofuranosyl-7H-pyrrolo-(2, 3-d)-pyrimidine-5-carboxamide, NSC 188491) for 24 hours. ATC code:---
Control untreated MCF-7 cell sample
MCF-7 cells untreated.

interferon-alpha-kinoid study 1 (240ug; 168d) / untreated whole blood sample

Relative Expression (log2-ratio):-1.447319
Number of Samples:2 / 43
Experimental interferon-alpha-kinoid study 1 (240ug; 168d)
Whole blood samples of patients with systemic lupus erythematosus after treatment with interferon-alpha-kinoid (INF-K; inactivated IFNa coupled keyhole limpet hemocyanin as carrier). Patients had 3 doses of 240ug INK-K at day 0, 7 and 58. Sample was taken 168 days after the first application of TNF-K. ATC code:---
Control untreated whole blood sample
Whole blood samples from healthy volunteers.