TOP TEN perturbations for NM_000020 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000020
Selected probe(set): 226950_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000020 (226950_at) across 5339 perturbations tested by GENEVESTIGATOR:

acute coronary syndrome study 1 (STEMI) / peripheral blood leukocyte sample (STEMI)

Relative Expression (log2-ratio):4.1104727
Number of Samples:4 / 4
Experimental acute coronary syndrome study 1 (STEMI)
Thrombus-derived leukocytes obtained from patients with ST-segment elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI). Leukocytes were derived from an occluding coronary thrombus, immediately after diagnostic angiography and identification of the culprit lesion, the presence of total coronary occlusion and angiographic signs of an occluding thrombus. The culprit coronary artery was intubated with a 6 F guiding catheter and wired with a 0.014 inch guide wire and prior to any subsequent interventional procedures, thrombi were removed from the site of coronary occlusion with an aspiration catheter and immersed in phosphate-buffered saline immediately.
Control peripheral blood leukocyte sample (STEMI)
Peripheral blood leukocytes derived from patients with ST-elevation myocardial infarction (STEMI).

stem cell differentiation study 41 (hES-T3 EB) / undifferentiated hES-T3 cell sample

Relative Expression (log2-ratio):2.9513311
Number of Samples:2 / 3
Experimental stem cell differentiation study 41 (hES-T3 EB)
Sample of embryonic bodies (EB) differentiated from human embryonic stem cells T3 with female karyotype.
Control undifferentiated hES-T3 cell sample
Undifferentiated human embryonic stem cells T3.

prostate cancer study 8 (p. canc) / prostate cancer study 8 (ptasc)

Relative Expression (log2-ratio):-2.884922
Number of Samples:3 / 2
Experimental prostate cancer study 8 (p. canc)
CD26+ FACS sorted prostate neoplasm cell (p. canc) samples from patients with primary prostate cancer collected after radical prostatectomy.
Control prostate cancer study 8 (ptasc)
CD90+ FACS sorted prostate tumor-associated stromal cell (ptasc) samples from patients with primary prostate cancer collected after radical prostatectomy.

non-small cell lung cancer study 8 (large cell carcinoma) / adjacent lung tissue

Relative Expression (log2-ratio):-2.813387
Number of Samples:18 / 65
Experimental non-small cell lung cancer study 8 (large cell carcinoma)
Primary tumor tissue sample obtained from the lung of patients large cell carcinoma.
Control adjacent lung tissue
Histological normal lung tissue samples from patients with lung cancer.

colorectal cancer study 4 (non-recurring) / adjacent colon tissue (non-recurring)

Relative Expression (log2-ratio):-2.784933
Number of Samples:76 / 18
Experimental colorectal cancer study 4 (non-recurring)
LCM-tumor tissue samples of patients who received resection after diagnosis of primary colorectal cancer. Patients did not develop metastatic recurrence during follow-up after surgery.
Control adjacent colon tissue (non-recurring)
Histologically normal colon tissue samples from patients with primary colorectal cancer collected after resection. Tissue was further extracted using laser-capture microdissection (LCM). No metastatic recurrence during follow-up.

lung adenocarcinoma study 3 (positive) / normal lung tissue

Relative Expression (log2-ratio):-2.622468
Number of Samples:7 / 3
Experimental lung adenocarcinoma study 3 (positive)
Primary lung adenocarcinoma samples, with bone marrow positive for early disseminated tumor cells (DTCs). The presence of DTCs can predict the subsequent occurrence of overt metastasis and survival in lung cancer.
Control normal lung tissue
Normal lung tissue.

human AB serum study 1 (intermediate) / IL-4; GM-CSF study 1 (intermediate)

Relative Expression (log2-ratio):2.57088
Number of Samples:3 / 7
Experimental human AB serum study 1 (intermediate)
Monocytes, cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS, and 500 U/ml IL-4 and 800 U/ml GM-CSF for 24 hours.
Control IL-4; GM-CSF study 1 (intermediate)
Monocytes, cultured with vehicle (DMSO/ethanol) and 500 U/ml IL-4 and 800 U/ml GM-CSF for 24 hours.

pancreatic islet study 3 (re-differentiated; PPRF; 2d) / normal pancreatic islet sample

Relative Expression (log2-ratio):2.5684729
Number of Samples:2 / 7
Experimental pancreatic islet study 3 (re-differentiated; PPRF; 2d)
Human pancreatic islets were isolated from a cadaveric donor. The population was 70% pure in mature islets, which was determined by dithizone staining. Islets cells were expanded for 6 weeks and re-differentiated for 2 days according to Pharmaceutical Production Research Facility (PPRF) Protocol. Re-differentiation phase: For induction of expanded cells to differentiate into islet-like cell clusters, the cells were seeded into 60 mm ultra-low attachment dishes in serum-free CMRL-1066 supplemented with 4 mM L-glutamine, 1% BSA, insulin (10 g/ml), transferrin (5.5 g/ml), sodium selenite (6.7ng/ml), and 1% antibiotic antimycotic solution at a density of 6.0 x 10EXP6 cells per dish. The induction medium was further added with islet neogenesis-associated protein (INGAP) peptide at a concentration of 1.0ug/ml.
Control normal pancreatic islet sample
Pancreatic islets were obtained from seven donors aged between 37 and 70 years and body mass index between 22 and 27. Functional islets were cultured in CMRL 1066 supplemented with 10% FCS, 1% glutamine, 5.6 mM glucose, 1 mM HEPES, 110 U/ml penicillin, and 110 g/ml streptomycin for 7 days or immediately processed after isolation.

lung adenocarcinoma study 3 / adjacent lung tissue

Relative Expression (log2-ratio):-2.5504322
Number of Samples:93 / 98
Experimental lung adenocarcinoma study 3
Primary adenocarcinoma samples from the lung of patients with different stages and smoking status.
Control adjacent lung tissue
Adjacent, histologically normal and non-neoplastic tissue sample from the lung of patients with primary adenocarcinoma of the lung. Patients had different stages and smoking status.

lung adenocarcinoma study 3 (negative) / normal lung tissue

Relative Expression (log2-ratio):-2.5219917
Number of Samples:9 / 3
Experimental lung adenocarcinoma study 3 (negative)
Primary lung adenocarcinoma samples, with bone marrow negative for early disseminated tumor cells (DTCs). The presence of DTCs can predict the subsequent occurrence of overt metastasis and survival in lung cancer.
Control normal lung tissue
Normal lung tissue.