TOP TEN perturbations for NM_000109 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000109
Selected probe(set): 203881_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000109 (203881_s_at) across 5392 perturbations tested by GENEVESTIGATOR:

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):-6.0765915
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

B-CLL study 11 (rolipram) / rolipram study 4 (normal T-cell; 20uM)

Relative Expression (log2-ratio):5.9065886
Number of Samples:4 / 4
Experimental B-CLL study 11 (rolipram)
Peripheral blood mononuclear cells (PBMCs) obtained from chronic lymphocytic leukemia (CLL) patients and in vitro treated with rolipram (20 uM, cyclic nucleotide phosphodiesterase (PDE4) inhibitor) for 4 hours. PBMCs? samples contained 90% CD19+ CD5+ B lineage CLL cells (B-CLL). Inclusion criteria: untreated CLL patients or at least 1 month after chemotherapy. Exclusion criteria: patients with active infections or other serious medical conditions or with white blood cell (WBC) counts of 15,000/l. ATC code:---
Control rolipram study 4 (normal T-cell; 20uM)
MACS purified T-cells from healthy donor peripheral blood treated with rolipram (20 uM, cyclic nucleotide phosphodiesterase (PDE4) inhibitor) for 4 hours. ATC code:---

glioma study 16 (LN-319) / normal astrocyte sample

Relative Expression (log2-ratio):-5.8978806
Number of Samples:2 / 3
Experimental glioma study 16 (LN-319)
Human glioma cell line LN319 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):-5.8299866
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

B-CLL study 11 (DMSO) / vehicle (DMSO) treated normal T-cell sample

Relative Expression (log2-ratio):5.7968273
Number of Samples:4 / 4
Experimental B-CLL study 11 (DMSO)
Peripheral blood mononuclear cells (PBMCs) obtained from chronic lymphocytic leukemia (CLL) patients and in vitro treated with vehicle (DMSO) for 4 hours. PBMCs? samples contained 90% CD19+ CD5+ B lineage CLL cells (B-CLL). Inclusion criteria: untreated CLL patients or at least 1 month after chemotherapy. Exclusion criteria: patients with active infections or other serious medical conditions or with white blood cell (WBC) counts of 15,000/l.
Control vehicle (DMSO) treated normal T-cell sample
MACS purified T-cells from healthy donor peripheral blood treated with vehicle (DMSO) for 4 hours.

breast cancer study 31 (MCF-7 1GX) / control virus infected MCF7 cell sample

Relative Expression (log2-ratio):5.5990047
Number of Samples:2 / 2
Experimental breast cancer study 31 (MCF-7 1GX)
Xenograft-derived MCF-7 cells isolated from primary tumor of breast in non-ovarioectomized female NCR/Nu/Nu nude mice. Four-week-old non-ovarioectomized female NCR/Nu/Nu nude mice were anesthetized by exposure to 3% isoflurane and injected into mammary fat pad with cells (MCF-7 cell line transfected with empty retroviral vector encoding neomycine resistance gene) suspended in 50 ul of 50% Matrigel. After 12 weeks, mice were sacrificed and primary xenograft tumors were processed. Tumor cells were isolated from primary tumor and expanded in culture for further use. It is not further specified whether the microarray analysis was performed on freshly isolated tumor cells or in vitro expanded cells.
Control control virus infected MCF7 cell sample
MCF7 cell line transfected with pZipneo retrovirus, which is an empty retroviral vector encoding neomycine resistance gene (NeoR). MCF7 cells were maintained in EMEM supplemented with 10% FBS and 20ug/ml insulin.

NCI-H358 / HCC827

Relative Expression (log2-ratio):4.8925424
Number of Samples:6 / 6
Experimental NCI-H358
Human bronchioloalveolar carcinoma derived cell line. Synonyms:H358; H-358 Cellosaurus code:
Control HCC827
Human primary cancer cell line derived from the lung of a patient with non-small-cell lung cancer. Synonyms:HCC-827; HCC0827 Cellosaurus code:

prostate cancer study 8 (p. canc) / prostate cancer study 8 (psfmc)

Relative Expression (log2-ratio):-4.592326
Number of Samples:3 / 5
Experimental prostate cancer study 8 (p. canc)
CD26+ FACS sorted prostate neoplasm cell (p. canc) samples from patients with primary prostate cancer collected after radical prostatectomy.
Control prostate cancer study 8 (psfmc)
CD49a+ FACS sorted prostate stromal fibromuscular cell (psfmc) samples from patients with primary prostate cancer collected after radical prostatectomy.

ovarian tumor study 16 / normal ovarian surface epithelial cell sample

Relative Expression (log2-ratio):-4.367304
Number of Samples:3 / 5
Experimental ovarian tumor study 16
Human epithelial tumor cell samples from the ovary of patients with papillary serous carcinoma. Samples were derived by laser capture microdissection (LCM).
Control normal ovarian surface epithelial cell sample
Human epithelial cell samples from histopathological normal and non-cancerous ovary tissue.

pancreatic cancer study 3 (ipmc) / normal pancreas tissue

Relative Expression (log2-ratio):-4.3421564
Number of Samples:6 / 6
Experimental pancreatic cancer study 3 (ipmc)
Intraductal papillary mucinous carcinoma from patients with pancreatic cancer.
Control normal pancreas tissue
Normal human pancreatic tissue.