TOP TEN perturbations for NM_000142 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000142
Selected probe(set): 204379_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000142 (204379_s_at) across 5392 perturbations tested by GENEVESTIGATOR:

p73 overexpr. study 1 (adenovirus) / control virus infected NCI-H1299 cell sample

Relative Expression (log2-ratio):5.7399263
Number of Samples:2 / 2
Experimental p73 overexpr. study 1 (adenovirus)
NCI-H1299 cells infected with adenovirus expressing TAp73beta for 5 hours. Cells were harvested after 80% transduction efficiency was reached, as monitored by GFP fluorescence.
Control control virus infected NCI-H1299 cell sample
NCI-H1299 cells infected with control adenovirus expressing GFP for 5 hours. Cells were harvested after 80% transduction efficiency was reached, as monitored by GFP fluorescence.

small cell lung cancer study 4 (1. gen. PDX) / small cell lung cancer study 4 (XCL from PDX)

Relative Expression (log2-ratio):5.7039604
Number of Samples:4 / 3
Experimental small cell lung cancer study 4 (1. gen. PDX)
A small cell lung cancer (SCLC) primary xenograft tumor tissue samples derived from three chemo-naive patients (LX22; LX33 and LX36; first generation-1. gen.). The discarded tissue obtained during bronchoscopy of SCLC patients was used to generate a xenograft. Aseptically resuspended tumour cells were injected s.c. in the flanks of nonobese diabetic/severe combined immunodeficient mice (NOD/SCID). The mice were sacrificed when the P0 tumors reached 1cm in diameter.
Control small cell lung cancer study 4 (XCL from PDX)
Xenograft-derived cell lines (XCL) established from a small cell lung carcinoma (SCLC) patient derived xenografts (PDX). The discarded tissue from three chemo-naive patients (LX22; LX33 and LX35) was obtained during bronchoscopy and used to generate a xenograft. Aseptically resuspended tumour cells were injected s.c. in the flanks of nonobese diabetic/severe combined immunodeficient mice. The mice were sacrificed when the P0 tumors reached 1cm in diameter. Obtained cells were used for conventional cell culture cultivation.

glioma study 17 (small cell glioblastoma; A2B5+) / non-tumor oligodendrocyte progenitor cell sample (cortex)

Relative Expression (log2-ratio):-5.6271305
Number of Samples:2 / 3
Experimental glioma study 17 (small cell glioblastoma; A2B5+)
Oligodendrocyte progenitor cells (OPC) isolated from high grade small cell glioblastoma (grade IV). OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen. Patients were 56 ? 3 years old males.
Control non-tumor oligodendrocyte progenitor cell sample (cortex)
Oligodendrocyte progenitor cells (OPC) isolated from cortical tissue, which was obtained from patients with epilepsy, but without any manifested brain cancer. OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen.

glioma study 17 (oligoastrocytoma; A2B5+) / non-tumor oligodendrocyte progenitor cell sample (cortex)

Relative Expression (log2-ratio):-5.4092607
Number of Samples:3 / 3
Experimental glioma study 17 (oligoastrocytoma; A2B5+)
Oligodendrocyte progenitor cells (OPC) isolated from low grade oligoastrocytoma (grade II). OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen. Patients were 37 ? 10 years old males.
Control non-tumor oligodendrocyte progenitor cell sample (cortex)
Oligodendrocyte progenitor cells (OPC) isolated from cortical tissue, which was obtained from patients with epilepsy, but without any manifested brain cancer. OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen.

small cell lung cancer study 4 (2. gen. PDX) / small cell lung cancer study 4 (1. gen. PDX)

Relative Expression (log2-ratio):-5.3536367
Number of Samples:4 / 4
Experimental small cell lung cancer study 4 (2. gen. PDX)
Xenografts (secondary) tumor tissue samples derived from a PDX-derived cell lines. A patient derived xenograft (PDX)-derived cell lines were grown under conventional tissue culture conditions for six months and then reimplanted to generate secondary xenografts (2. gen.).
Control small cell lung cancer study 4 (1. gen. PDX)
A small cell lung cancer (SCLC) primary xenograft tumor tissue samples derived from three chemo-naive patients (LX22; LX33 and LX36; first generation-1. gen.). The discarded tissue obtained during bronchoscopy of SCLC patients was used to generate a xenograft. Aseptically resuspended tumour cells were injected s.c. in the flanks of nonobese diabetic/severe combined immunodeficient mice (NOD/SCID). The mice were sacrificed when the P0 tumors reached 1cm in diameter.

renal cell carcinoma study 6 (chromophobe type) / normal kidney tissue

Relative Expression (log2-ratio):-5.1679173
Number of Samples:4 / 2
Experimental renal cell carcinoma study 6 (chromophobe type)
Tumor tissue samples from the kidney of patients with chromophobe renal cell carcinoma (cRCC).
Control normal kidney tissue
Normal fetal kidney tissue samples.

male infertility study 1 (mJS7) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):5.0233335
Number of Samples:4 / 7
Experimental male infertility study 1 (mJS7)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained early but no late spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS7.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

lung adenocarcinoma study 9 (metastase; lymph node) / lung adenocarcinoma study 9 (metastase; brain)

Relative Expression (log2-ratio):-5.020563
Number of Samples:3 / 6
Experimental lung adenocarcinoma study 9 (metastase; lymph node)
Metastatic tumor tissue obtained from the lymph node of patients with primary lung adenocarcinoma.
Control lung adenocarcinoma study 9 (metastase; brain)
Metastatic tumor tissue obtained from the brain of patients with primary lung adenocarcinoma.

glioma study 17 (oligodendroglioma; A2B5+) / non-tumor oligodendrocyte progenitor cell sample (cortex)

Relative Expression (log2-ratio):-4.980402
Number of Samples:3 / 3
Experimental glioma study 17 (oligodendroglioma; A2B5+)
Oligodendrocyte progenitor cells (OPC) isolated from low grade oligodendroglioma (grade II). OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen. Patients were 39 ? 9 years old.
Control non-tumor oligodendrocyte progenitor cell sample (cortex)
Oligodendrocyte progenitor cells (OPC) isolated from cortical tissue, which was obtained from patients with epilepsy, but without any manifested brain cancer. OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen.

male infertility study 1 (mJS5) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):4.9775
Number of Samples:8 / 7
Experimental male infertility study 1 (mJS5)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained spermatocytes but no spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS5.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.