TOP TEN perturbations for NM_000145 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000145
Selected probe(set): 211201_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000145 (211201_at) across 5392 perturbations tested by GENEVESTIGATOR:

male infertility study 1 (juvenile; Ad-) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):2.022727
Number of Samples:2 / 8
Experimental male infertility study 1 (juvenile; Ad-)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained very low level of A-dark (Ad-) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

male infertility study 1 (juvenile; Ad+) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):1.6629324
Number of Samples:5 / 8
Experimental male infertility study 1 (juvenile; Ad+)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained typical level of A-dark (Ad+) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

stem cell differentiation study 53 (differentiated; DETA-NO) / stem cell differentiation study 53 (differentiated)

Relative Expression (log2-ratio):0.94850636
Number of Samples:3 / 3
Experimental stem cell differentiation study 53 (differentiated; DETA-NO)
Differentiated human embryonic stem cell line HS181 from the passage 72-74. Cells were differentiated due to withdrawal of bovine fibroblast growth factor (bFGF) from the culture medium. Cells were also treated with 2uM Diethylenetriamine/nitric oxide adduct (DETA/NO). Culture was maintained for 6 days.
Control stem cell differentiation study 53 (differentiated)
Differentiated human embryonic stem cell line HS181 from the passage 72-74. Cells were differentiated due to withdrawal of bovine fibroblast growth factor (bFGF) from the culture medium. Culture was maintained for 6 days.

male infertility study 1 (mJS5) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):-0.87451935
Number of Samples:8 / 7
Experimental male infertility study 1 (mJS5)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained spermatocytes but no spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS5.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

stem cell differentiation study 53 (differentiated) / stem cell differentiation study 53 (undifferentiated; bFGF)

Relative Expression (log2-ratio):-0.86573124
Number of Samples:3 / 3
Experimental stem cell differentiation study 53 (differentiated)
Differentiated human embryonic stem cell line HS181 from the passage 72-74. Cells were differentiated due to withdrawal of bovine fibroblast growth factor (bFGF) from the culture medium. Culture was maintained for 6 days.
Control stem cell differentiation study 53 (undifferentiated; bFGF)
Undifferentiated human embryonic stem cell line HS181 from the passage 72-74. Cells were maintained undifferentiated by adding 8ng/ul of bovine fibroblast growth factor (bFGF) to the culture medium. Culture was maintained for 6 days.

male infertility study 1 (mJS7) / male infertility study 1 (mJS2)

Relative Expression (log2-ratio):-0.85980606
Number of Samples:4 / 7
Experimental male infertility study 1 (mJS7)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained early but no late spermatids. Tissue samples were classified based on modified Johnsen score (mJS) as mJS7.
Control male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.

brefeldin A study 1 (0.5ug/ml; HCT 116) / untreated HCT 116 cell sample

Relative Expression (log2-ratio):-0.8573737
Number of Samples:3 / 3
Experimental brefeldin A study 1 (0.5ug/ml; HCT 116)
Human colon carcinoma cell line HCT116 was treated with 0.5 ug/ml brefeldin-A for 24 hours in McCOYs 5A medium supplemented with 10% heat inactivated FBS. ATC code:---
Control untreated HCT 116 cell sample
Human colon carcinoma cell line HCT116 was grown in McCOYs 5A medium supplemented with 10% heat inactivated FBS.

engineered skin substitute study 2 (intermediate) / engineered skin substitute study 1 (intermediate)

Relative Expression (log2-ratio):0.8436947
Number of Samples:6 / 3
Experimental engineered skin substitute study 2 (intermediate)
Human skin substitutes tissue samples collected after 14 and 28 days after transplantation to athymic mice.
Control engineered skin substitute study 1 (intermediate)
Human skin substitutes tissue samples collected after 7 days in culture.

medulloblastoma study 2 / non-tumor brain tissue

Relative Expression (log2-ratio):0.8392019
Number of Samples:4 / 9
Experimental medulloblastoma study 2
Primary tumor tissue sample from the infratentorial brain of pediatric patients with large-cell anaplastic medulloblastoma.
Control non-tumor brain tissue
Histologically normal brain tissue at rapid autopsy from patients who died from atypical teratoid/rhabdoid tumor.

engineered skin substitute study 2 (late) / engineered skin substitute study 1 (late)

Relative Expression (log2-ratio):0.74654675
Number of Samples:5 / 3
Experimental engineered skin substitute study 2 (late)
Human skin substitutes tissue samples collected after 42 and 56 days after transplantation to athymic mice.
Control engineered skin substitute study 1 (late)
Human skin substitutes tissue samples collected after 14 days in culture.