TOP TEN perturbations for NM_000201 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000201
Selected probe(set): 202637_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000201 (202637_s_at) across 5339 perturbations tested by GENEVESTIGATOR:

IFN-g study 9 (24h) / untreated epidermal keratinocyte sample

Relative Expression (log2-ratio):5.93052
Number of Samples:3 / 6
Experimental IFN-g study 9 (24h)
Primary keratinocytes treated with interferon gamma (IFN-g) for 24 hours. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency. Cultures were starved of growth factors in nonsupplemented M154 medium for 24 hours, and afterwards stimulated with recombinant human IFN-g for 24 hours.
Control untreated epidermal keratinocyte sample
Untreated primary epidermal keratinocytes. Monolayer normal human keratinocyte (NHK) cultures were established and used in the second or third passage. Cultures were grown to 40 or 80% confluence, or maintained to 4 days postconfluency.

PMA study 6 (500ng/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):5.5557604
Number of Samples:3 / 17
Experimental PMA study 6 (500ng/ml)
Bronchial epithelial cells (NHBE) treated with tetradecanoylphorbol acetate (500ng/ml; vendor: Sigma / catalog number: P1585 / catalog name: Phorbol 12-myristate 13-acetate [16561-29-8]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:---
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

IFN-g study 3 / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):5.4381876
Number of Samples:2 / 17
Experimental IFN-g study 3
Bronchial epithelial cells (NHBE) treated with 100 ng/ml recombinant human interferon gamma (IFN-g; vendor: PeproTech / catalog number: 300-02 / catalog name: IFN-?) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

TNF-?; TGF-?2 study 1 (intermediate) / untreated ARPE-19 cell sample

Relative Expression (log2-ratio):5.152193
Number of Samples:6 / 3
Experimental TNF-?; TGF-?2 study 1 (intermediate)
ARPE-19 retina pigment epithelial cell samples treated with TNF-? (10 ng/ml) and TGF-?2 (5 ng/ml). Samples were taken 16 and 24 hours after treatment.
Control untreated ARPE-19 cell sample
Untreated ARPE-19 retina pigment epithelial cell samples harvested before adding TNF-? (10 ng/ml) and TGF-?2 (5 ng/ml).

polyinosinic-polycytidylic acid study 1 (10ug/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):4.9730024
Number of Samples:3 / 17
Experimental polyinosinic-polycytidylic acid study 1 (10ug/ml)
Bronchial epithelial cells (NHBE) treated with polyinosinic-polycytidylic acid (Poly(I:C); 10ug/ml; vendor: InvivoGen / catalog number: tlrl-pic / catalog name: Poly(I:C) High Molecular Weight [31852-29-6]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

TNF-?; TGF-?2 study 1 (late) / untreated ARPE-19 cell sample

Relative Expression (log2-ratio):4.940875
Number of Samples:6 / 3
Experimental TNF-?; TGF-?2 study 1 (late)
ARPE-19 retina pigment epithelial cell samples treated with TNF-? (10 ng/ml) and TGF-?2 (5 ng/ml). Samples were taken 42 and 60 hours after treatment.
Control untreated ARPE-19 cell sample
Untreated ARPE-19 retina pigment epithelial cell samples harvested before adding TNF-? (10 ng/ml) and TGF-?2 (5 ng/ml).

TNF-?; TGF-?2 study 1 (early) / untreated ARPE-19 cell sample

Relative Expression (log2-ratio):4.51987
Number of Samples:5 / 3
Experimental TNF-?; TGF-?2 study 1 (early)
ARPE-19 retina pigment epithelial cell samples treated with TNF-? (10 ng/ml) and TGF-?2 (5 ng/ml). Samples were taken 1 hour and 6 hours after treatment.
Control untreated ARPE-19 cell sample
Untreated ARPE-19 retina pigment epithelial cell samples harvested before adding TNF-? (10 ng/ml) and TGF-?2 (5 ng/ml).

EBNA2 overexpr. study 1 (4h) / control virus transfected EREB2-5 cell sample (24h)

Relative Expression (log2-ratio):4.4350805
Number of Samples:3 / 3
Experimental EBNA2 overexpr. study 1 (4h)
EREB2-5 cell line stably transfected with the chimeric Epstein-Barr virus-encoded nuclear antigen 2 (EBNA2) and control expression plasmid coding for bacterial chloramphenicol acetyltransferase (CAT) gene and . CAT was used as a negative control. Briefly, before the CAT and EBNA2 induction, cells were maintained in RPMI media and deprived of estrogen for 3 days before doxycycline and estrogen was added. Expression of EBNA2 was induced after addition of estrogen and expression of CAT was induced by the addition of doxycycline to a final concentration of 100 ng/mL medium. Doxycycline induced cells were harvested 4 hours after induction. Because EBNA2 is essential for the proliferation of EBV-infected cells, EREB2-5 cells grow only in the presence of estrogen. Before the preparation of RNA, NGFR cells were purified by MACS separation to enrich the cells with a transcriptional response to doxycycline.
Control control virus transfected EREB2-5 cell sample (24h)
EREB2-5 cell line stably transfected with control expression plasmid coding for bacterial chloramphenicol acetyltransferase (CAT) gene. CAT was used as a negative control. Briefly, before the CAT induction, cells were maintained in RPMI media and deprived of estrogen for 3 days before doxycycline was added. Expression of CAT was induced by the addition of doxycycline to a final concentration of 100 ng/mL medium. Doxycycline induced cells were harvested 24 hours after induction. Estrogen withdrawal leads to the inactivation of EBNA2, resulting in cell cycle arrest. Before the preparation of RNA, NGFR cells were purified by MACS separation to enrich the cells with a transcriptional response to doxycycline.

IL-1b; IFN-g study 1 (8hrs) / untreated pancreatic islet sample (8hrs)

Relative Expression (log2-ratio):4.226219
Number of Samples:2 / 3
Experimental IL-1b; IFN-g study 1 (8hrs)
Pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in presence of human recombinant IL-1b (25 units/ml) and IFN-g (1,000 units/ml) for 8 hours.
Control untreated pancreatic islet sample (8hrs)
Untreated pancreatic islets isolated by collagenase digestion from brain-dead organ donors and cultured 3-5 days in RPMI 1640 medium containing 5.6 mM glucose, 10% FCS and 2 mM L-glutamine. Thereafter, islets were cultured in fresh media and harvested after 8 hours.

TNF-? study 10 (150pg/ml) / normal umbilical vein endothelial cell sample

Relative Expression (log2-ratio):4.1204176
Number of Samples:3 / 3
Experimental TNF-? study 10 (150pg/ml)
Human umbilical vein endothelial cells (HUVEC) treated for 4 hours with TNFalpha (150 pg/ml). HUVEC were cultured as monolayer on collagen A-coated cell culture plates, in standard endothelial growth medium. 1 day after confluency cells were starved overnight in endothelial growth medium containing 0.5% FCS and exposed for 4 hours to TNF-?. Primary cells were purchased from PromoCell.
Control normal umbilical vein endothelial cell sample
Human umbilical vein endothelial cells (HUVEC) cultured as monolayer on collagen A-coated cell culture plates, in standard endothelial growth medium. 1 day after confluency cells were starved overnight in HUVEC medium containing 0.5% FCS. Primary cells were purchased from PromoCell.