TOP TEN perturbations for NM_000212 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000212
Selected probe(set): 204627_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000212 (204627_s_at) across 5392 perturbations tested by GENEVESTIGATOR:

galectin-1 study 1 / vehicle (DTT/polym. B) treated monocyte sample

Relative Expression (log2-ratio):5.851162
Number of Samples:3 / 3
Experimental galectin-1 study 1
Monocytes were plated in 12-well plates and differentiated in RPMI 1640 containing 10% FBS plus 50 ng/ml (>500 U/ml) GM-CSF and 100 ng/ml (>500 U/ml) IL-4 for 5 days. At day 5, galectin-1 (20 ?M) in the presence of 10 ?g/ml polymyxin B was added. At 18 h after treatment, total RNA was isolated.
Control vehicle (DTT/polym. B) treated monocyte sample
Monocytes were plated in 12-well plates and differentiated in RPMI 1640 containing 10% FBS plus 50 ng/ml (>500 U/ml) GM-CSF and 100 ng/ml (>500 U/ml) IL-4 for 5 days. At day 5, vehicle consisting of equivalent volume of 8 mM DTT (galectin-1 storage buffer) and 10 ?g/ml polymyxin B was added. At 18 h after treatment, total RNA was isolated.

LPS study 1 / vehicle (DTT/polym. B) treated monocyte sample

Relative Expression (log2-ratio):4.304306
Number of Samples:3 / 3
Experimental LPS study 1
Monocytes were plated in 12-well plates and differentiated in RPMI 1640 containing 10% FBS plus 50 ng/ml (>500 U/ml) GM-CSF and 100 ng/ml (>500 U/ml) IL-4 for 5 days. At day 5, Lipopolysaccharide (LPS; 100 ng/ml) was added. At 18 h after treatment, total RNA was isolated. ATC code:---
Control vehicle (DTT/polym. B) treated monocyte sample
Monocytes were plated in 12-well plates and differentiated in RPMI 1640 containing 10% FBS plus 50 ng/ml (>500 U/ml) GM-CSF and 100 ng/ml (>500 U/ml) IL-4 for 5 days. At day 5, vehicle consisting of equivalent volume of 8 mM DTT (galectin-1 storage buffer) and 10 ?g/ml polymyxin B was added. At 18 h after treatment, total RNA was isolated.

IL-4; GM-CSF study 1 (intermediate) / untreated monocyte sample

Relative Expression (log2-ratio):3.110382
Number of Samples:7 / 12
Experimental IL-4; GM-CSF study 1 (intermediate)
Monocytes, cultured with vehicle (DMSO/ethanol) and 500 U/ml IL-4 and 800 U/ml GM-CSF for 24 hours.
Control untreated monocyte sample
Freshly isolated human monocytes from healthy donors.

E. coli study 2 / unstimulated, normal monocyte-derived macrophage sample

Relative Expression (log2-ratio):3.052043
Number of Samples:5 / 7
Experimental E. coli study 2
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours with 2.5 ? 105 heat-killed E. coli.
Control unstimulated, normal monocyte-derived macrophage sample
Monocyte-derived macrophage samples derived from healthy control subjects cultured for 4 hours unstimulated.

prostate cancer study 8 (p. canc) / prostate cancer study 8 (ptasc)

Relative Expression (log2-ratio):-3.0249462
Number of Samples:3 / 2
Experimental prostate cancer study 8 (p. canc)
CD26+ FACS sorted prostate neoplasm cell (p. canc) samples from patients with primary prostate cancer collected after radical prostatectomy.
Control prostate cancer study 8 (ptasc)
CD90+ FACS sorted prostate tumor-associated stromal cell (ptasc) samples from patients with primary prostate cancer collected after radical prostatectomy.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):3.017005
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

TGF-? study 9 / untreated PANC-1 cell sample

Relative Expression (log2-ratio):3.0042028
Number of Samples:3 / 3
Experimental TGF-? study 9
PANC-1 cells were grown to 70% confluency. Thereafter the cells were cultured for one day in serum-free conditions and then incubated for 48 hours with fresh media containing 5nM TGF-?. Before harvesting, cells were washed 3x with PBS and lysed by direct addition of TRIzol reagent for RNA extraction.
Control untreated PANC-1 cell sample
PANC-1 cells were grown to 70% confluency. Thereafter the cells were cultured for one day in serum-free conditions and then incubated for 48 hours with plain fresh media. Before harvesting, cells were washed 3x with PBS and lysed by direct addition of TRIzol reagent for RNA extraction.

ALL study 2 (15d) / ALL study 2 (0d)

Relative Expression (log2-ratio):2.928112
Number of Samples:45 / 129
Experimental ALL study 2 (15d)
Bone marrow samples from children with de novo acute lymphoblastic leukemia. Samples were taken 15 days after remission-induction therapy (RIT).
Control ALL study 2 (0d)
Bone marrow samples from children with de novo acute lymphoblastic leukemia. Samples were taken before remission-induction therapy (RIT).

ALL study 2 (33d) / ALL study 2 (0d)

Relative Expression (log2-ratio):2.922904
Number of Samples:58 / 129
Experimental ALL study 2 (33d)
Bone marrow samples from children with de novo acute lymphoblastic leukemia. Samples were taken 33 days after remission-induction therapy (RIT).
Control ALL study 2 (0d)
Bone marrow samples from children with de novo acute lymphoblastic leukemia. Samples were taken before remission-induction therapy (RIT).

TGF-? study 11 / untreated HCC827 cell sample

Relative Expression (log2-ratio):2.847599
Number of Samples:3 / 3
Experimental TGF-? study 11
HCC827 cells were cultured for 3-5 weeks in the presence of 2ng/ml of TGF-?, to induce epithelial-mesenchymal transition (EMT). 24 hours prior analysis cells were re-seeded without TGF-?.
Control untreated HCC827 cell sample
HCC827 cells grown in standard media.