TOP TEN perturbations for NM_000222 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000222
Selected probe(set): 205051_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000222 (205051_s_at) across 5339 perturbations tested by GENEVESTIGATOR:

hepatocyte (ESC) / Hep-G2

Relative Expression (log2-ratio):5.947995
Number of Samples:8 / 9
Experimental hepatocyte (ESC)
Hepatocyte-like cells differentiated from embryonic stem cells (ESC)
Control Hep-G2
Human primary cancer cell line derived from the liver of a patient with hepatocellular carcinoma. Synonyms:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 Cellosaurus code:

hepatocyte (ESC) / HepaRG

Relative Expression (log2-ratio):5.660303
Number of Samples:8 / 12
Experimental hepatocyte (ESC)
Hepatocyte-like cells differentiated from embryonic stem cells (ESC)
Control HepaRG
Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code:

TC-71 / TC-32

Relative Expression (log2-ratio):-5.2111797
Number of Samples:6 / 6
Experimental TC-71
Human primary cancer cell line derived from the humerus of a patient with Ewing sarcoma. Synonyms:TC71; GM11654 Cellosaurus code:
Control TC-32
Human primary cancer cell line derived from the unspecified origin of a patient with Ewing?s sarcoma. Synonyms:TC32 Cellosaurus code:

basal cell carcinoma study 3 / normal epidermal keratinocytes

Relative Expression (log2-ratio):5.1096544
Number of Samples:4 / 2
Experimental basal cell carcinoma study 3
Primary tumor tissue from the eyelid of patients with basal cell carcinoma (BCC).
Control normal epidermal keratinocytes
Normal human epidermal keratinocytes (NHEK) (Kurabo Ind., Ltd., Osaka, Japan) cultured in HuMedia-KB2 medium at 37?C in humidified air containing 5% CO2.

TGF-? study 5 (late) / untreated A549 cell sample

Relative Expression (log2-ratio):-4.5326185
Number of Samples:3 / 3
Experimental TGF-? study 5 (late)
Human A549 cells were treated with 5ng/ml porcine TGF-? after serum-starving for 24h. Samples were taken 72 hours after TGF-? treatment.
Control untreated A549 cell sample
A549 cells were grown for 24 hours. Samples were taken immediately before TGF-? treatment.

TGF-? study 5 (intermediate) / untreated A549 cell sample

Relative Expression (log2-ratio):-4.3765564
Number of Samples:9 / 3
Experimental TGF-? study 5 (intermediate)
Human A549 cells were treated with 5ng/ml porcine TGF-? after serum-starving for 24h. Samples were taken 8, 16, 24 hours after TGF-? treatment.
Control untreated A549 cell sample
A549 cells were grown for 24 hours. Samples were taken immediately before TGF-? treatment.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):-4.308736
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):4.1953087
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

breast cancer study 5 / normal mammary gland tissue

Relative Expression (log2-ratio):-4.1832657
Number of Samples:20 / 7
Experimental breast cancer study 5
Breast non-BLC (sporadic basal-like cancer) tumor samples.
Control normal mammary gland tissue
Normal breast tissue samples.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)

Relative Expression (log2-ratio):4.0805635
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (confluent)
Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert.