TOP TEN perturbations for NM_000236 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000236
Selected probe(set): 206606_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000236 (206606_at) across 5392 perturbations tested by GENEVESTIGATOR:

RAF1 depletion study 1 / mock transfected SW-480 cell sample

Relative Expression (log2-ratio):4.262274
Number of Samples:6 / 6
Experimental RAF1 depletion study 1
RNAi-mediated gene knockdown of RAF1 in colorectal cancer cell line SW-480.
Control mock transfected SW-480 cell sample
SW-480 colorectal cell line sample transduced with non-targeting pLKO_NonTarget vector.

RAF1 depletion study 1 / control vector transfected SW-480 cell sample

Relative Expression (log2-ratio):3.8948288
Number of Samples:6 / 6
Experimental RAF1 depletion study 1
RNAi-mediated gene knockdown of RAF1 in colorectal cancer cell line SW-480.
Control control vector transfected SW-480 cell sample
SW-480 colorectal cell line sample transduced with empty vector pLKO.

SW620 / SW480

Relative Expression (log2-ratio):2.694726
Number of Samples:62 / 63
Experimental SW620
Human metastatic cancer cell line derived from the lymph node of a patient with adenocarcinoma of the colon. Synonyms:SW-620; SW 620; SW.620 Cellosaurus code:
Control SW480
Human primary cancer cell line derived from the colon of a male patient with adenocarcinoma. Synonyms:SW-480; SW 480; SW480E Cellosaurus code:

colchicine study 7 (4000uM) / vehicle (medium) treated hepatocyte sample

Relative Expression (log2-ratio):-2.6798687
Number of Samples:2 / 2
Experimental colchicine study 7 (4000uM)
Hepatocytes treated with compound: colchicine (4000uM; CHEMBL107) for 24 hours. ATC code:
Control vehicle (medium) treated hepatocyte sample
Hepatocytes treated with vehicle (medium) for 24 hours.

4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone study 1 (7500 uM; HepaRG) / vehicle (DMSO) treated HepaRG cell sample

Relative Expression (log2-ratio):-2.5700693
Number of Samples:3 / 12
Experimental 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone study 1 (7500 uM; HepaRG)
HepaRG cells exposed to 7500 uM 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (dissolved in 0.5% v/v DMSO) for 72 hours. Cells were cultivated in DMSO-containing medium between days 13 - 19 to allow them to differentiate into hepatocyte-like cells and become fully functional. Chemical was added at day 19 of cultivation. Cells were treated with the IC10 determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (number of replicates, n = 3). ATC code:---
Control vehicle (DMSO) treated HepaRG cell sample
HepaRG cells treated with vehicle (DMSO 0.5% v/v) for 72 hours. Firstly, cells were cultivated in DMSO-containing medium between days 13 - 19 to allow them to differentiate into hepatocyte-like cells and become fully functional. Then the vehicle was added.

HCC study 9 (alcohol; HBV) / HCC study 9 (HBV)

Relative Expression (log2-ratio):2.5526972
Number of Samples:3 / 10
Experimental HCC study 9 (alcohol; HBV)
Liver tissue biopsy sample from patients with alcohol abuse and Hepatitis B infection related hepatocellular carcinoma (HCC) after resection.
Control HCC study 9 (HBV)
Liver tissue biopsy sample from patients with Hepatitis B infection related hepatocellular carcinoma (HCC) after resection.

PMA study 8 (0.11 uM; hES-Heps) / vehicle (DMSO) treated hES-Heps cell sample

Relative Expression (log2-ratio):-2.3161526
Number of Samples:3 / 8
Experimental PMA study 8 (0.11 uM; hES-Heps)
hES-Heps cells exposed to 0.11 uM PMA (dissolved in 0.5% v/v DMSO) for 72 hours. Synonyms: phorbol-12-myristat-13-acetate (PMA), tetradecanoylphorbol-acetate (TPA), tetradecanoyl phorbol acetate, 12-O-tetradecanoylphorbol-13-acetate. Chemical was added at day 22 of differentiation. Cells were treated with the IC10 determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (number of replicates, n = 3). ATC code:---
Control vehicle (DMSO) treated hES-Heps cell sample
hES-Heps cells treated with vehicle (DMSO 0.5% v/v) for 72 hours. Vehicle was added at day 22 of differentiation.

sulindac study 5 (3000uM) / vehicle (medium) treated hepatocyte sample

Relative Expression (log2-ratio):-2.3145924
Number of Samples:2 / 2
Experimental sulindac study 5 (3000uM)
Hepatocytes treated with compound: sulindac (3000uM; CHEMBL15770) for 24 hours. ATC code:
Control vehicle (medium) treated hepatocyte sample
Hepatocytes treated with vehicle (medium) for 24 hours.

HCC study 9 (HBV) / normal liver tissue

Relative Expression (log2-ratio):-2.2768326
Number of Samples:10 / 10
Experimental HCC study 9 (HBV)
Liver tissue biopsy sample from patients with Hepatitis B infection related hepatocellular carcinoma (HCC) after resection.
Control normal liver tissue
Normal, non-tumor liver tissue.

furosemide study 5 (2500uM) / vehicle (medium) treated hepatocyte sample

Relative Expression (log2-ratio):-2.2701626
Number of Samples:2 / 2
Experimental furosemide study 5 (2500uM)
Hepatocytes treated with compound: furosemide (2500uM; CHEMBL35) for 24 hours. ATC code:
Control vehicle (medium) treated hepatocyte sample
Hepatocytes treated with vehicle (medium) for 24 hours.