TOP TEN perturbations for NM_000258 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000258
Selected probe(set): 205589_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000258 (205589_at) across 5339 perturbations tested by GENEVESTIGATOR:

exercise study 33 (OUT; fast twitch; pre-exerc.) / exercise study 33 (YUT; fast twitch; pre-exerc.)

Relative Expression (log2-ratio):-4.478629
Number of Samples:2 / 4
Experimental exercise study 33 (OUT; fast twitch; pre-exerc.)
Fast twitch myosin heavy chain muscle fibers (MHC) IIa were obtained from vastus lateralis muscle. Biopsies were taken from old untrained (OUT) women (85?1 years) before the first exercise. Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.
Control exercise study 33 (YUT; fast twitch; pre-exerc.)
Fast twitch myosin heavy chain muscle fibers (MHC) IIa were obtained from vastus lateralis muscle. Biopsies were taken from young untrained (YUT) women (23?2 years old) before the first exercise. Fibers were separated under the light microscope and MHC isoform was determinated based on SDS-PAGE analysis. Exclusion criteria: acute/chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin or non-insulin-dependent diabetes, abnormal blood, or urine chemistries, arthritis, a history of neuromuscular problems, tobacco smoking. Individuals, that had ever completed any formal exercise programs or physical activity outside of their activities of daily living, were excluded as well.

spinal cord injury study 1 (5d) / spinal cord injury study 1 (2d)

Relative Expression (log2-ratio):-3.8017492
Number of Samples:10 / 9
Experimental spinal cord injury study 1 (5d)
Vastus lateralis samples derived from spinal cord injury patients five days post-injury.
Control spinal cord injury study 1 (2d)
Vastus lateralis samples derived from spinal cord injury patients two days post-injury.

kidney transplantation study 9 (late) / normal kidney transplant

Relative Expression (log2-ratio):3.1018534
Number of Samples:2 / 13
Experimental kidney transplantation study 9 (late)
Human renal allograft biopsies from deceased donors obtained 24 months after transplantation.
Control normal kidney transplant
Human renal allograft biopsies from deceased donors obtained at implantation prior to revascularization.

embryonic development study 1 (7wk) / embryonic development study 1 (4wk)

Relative Expression (log2-ratio):-2.4369888
Number of Samples:3 / 3
Experimental embryonic development study 1 (7wk)
Human embryos at 7th week after fertilization. Embryos were obtained after therapeutic termination of pregnancy with mifepristone.
Control embryonic development study 1 (4wk)
Human embryos at 4th week after fertilization. Embryos were obtained after therapeutic termination of pregnancy with mifepristone.

rheumatoid arthritis study 27 / normal synovial membrane tissue

Relative Expression (log2-ratio):-2.3009052
Number of Samples:16 / 7
Experimental rheumatoid arthritis study 27
Synovial membrane samples from end-stage rheumatoid arthritis patients undergoing surgery.
Control normal synovial membrane tissue
Synovial membrane samples obtained by fine-needle arthroscopy from healthy subjects.

hepatocyte-like cell differentiation study 1 (15d) / hepatocyte-like cell differentiation study 1 (5d)

Relative Expression (log2-ratio):2.1745367
Number of Samples:3 / 3
Experimental hepatocyte-like cell differentiation study 1 (15d)
Hepatocyte-like cells differentiated from human embryonic stem cells (ES, WA09), 15 days after the onset of differentiation. This stage is approximately corresponding to immature hepatocytes. Human H9 (WA09) ES cells were routinely cultured under low oxygen conditions (4% O2/5% CO2) in human ES cell media (DMEM/F12 medium supplemented with 20% knockout serum replacement, non essential amino acids, glutamine, penicillin/streptomycin and bFGF (4ng/ml) on Matrigel coated plates using MEF-conditioned human ES cell media. Differentiation was initiated by culture for 5 days with 100ng/ml Activin A in RPMI/B27 medium under ambient oxygen/5%CO2 followed by 5 days with 20ng/ml BMP4 /10ng/ml FGF-2 in RPMI/B27 under 4%O2/5%CO2, then 5 days with 20ng/ml HGF in RPMI/B27 supplement under 4%O2/5%CO2.
Control hepatocyte-like cell differentiation study 1 (5d)
Hepatocyte-like cell differentiated from human pluripotent, embryonic stem cell line (ES, WA09). ES cells were grown in human ES cell media DMEM/F12 and differentiated to definitive endoderm for 5 days.

embryonic development study 1 (8wk) / embryonic development study 1 (4wk)

Relative Expression (log2-ratio):-2.166708
Number of Samples:3 / 3
Experimental embryonic development study 1 (8wk)
Human embryos at 8th week after fertilization. Embryos were obtained after therapeutic termination of pregnancy with mifepristone.
Control embryonic development study 1 (4wk)
Human embryos at 4th week after fertilization. Embryos were obtained after therapeutic termination of pregnancy with mifepristone.

sepsis-ind. mult. organ failure study 1 / normal vastus lateralis muscle tissue

Relative Expression (log2-ratio):-1.8344145
Number of Samples:12 / 8
Experimental sepsis-ind. mult. organ failure study 1
Muscle biopsies from patients suffering from sepsis-induced multiple organ failure (MOF), treated in the intensive care unit. Biopsies were obtained from the lateral portion of the vastus lateralis muscle, 10-20 cm above the knee, using a Bergstroem biopsy needle after local anesthesia.
Control normal vastus lateralis muscle tissue
Muscle biopsies from control patients undergoing elective surgery. Biopsies were obtained from the lateral portion of the vastus lateralis muscle, 10-20 cm above the knee, using a Bergstroem biopsy needle just after sedation.

MSHRIe002-A-SOX17 / MSHRIe002-A-con

Relative Expression (log2-ratio):1.6733379
Number of Samples:4 / 2
Experimental MSHRIe002-A-SOX17
Human embryonic stem cells overexpressing SOX17. Cells were generated by two stable transfections. Firstly, cells were transfected with the Cre-inducible expression vector for SOX7. In the second step, electroporation with a CRE recombinase vector (pCAGGS-NLS-CRE) and selection with puromycin was performed. Parental cell line:: MSHRIe002-A Synonyms:SOX17 O/E HESC
Control MSHRIe002-A-con
Human embryonic stem cells MSHRIe002-A modified by stable control transfection. Parental cell line:: MSHRIe002-A, CA2

glioma study 17 (anaplastic astrocytoma; A2B5+) / non-tumor oligodendrocyte progenitor cell sample (cortex)

Relative Expression (log2-ratio):-1.6529207
Number of Samples:2 / 3
Experimental glioma study 17 (anaplastic astrocytoma; A2B5+)
Oligodendrocyte progenitor cells (OPC) isolated from high grade anaplastic astrocytoma (grade III). OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen. Patients were 45 ? 18 years old.
Control non-tumor oligodendrocyte progenitor cell sample (cortex)
Oligodendrocyte progenitor cells (OPC) isolated from cortical tissue, which was obtained from patients with epilepsy, but without any manifested brain cancer. OPC were isolated using magnetic-activated cell sorting (MACS) with antibodies against A2B5 antigen.