TOP TEN perturbations for NM_000272 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000272
Selected probe(set): 238843_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000272 (238843_at) across 5392 perturbations tested by GENEVESTIGATOR:

colorectal cancer study 43 (metastase; brain) / colorectal cancer study 43 (metastase; lung)

Relative Expression (log2-ratio):-1.9460993
Number of Samples:2 / 5
Experimental colorectal cancer study 43 (metastase; brain)
Metastatic tumor tissue obtained from the brain of patients with primary colorectal adenocarcinoma.
Control colorectal cancer study 43 (metastase; lung)
Metastatic tumor tissue obtained from the lung of patients with primary colon adenocarcinoma.

male infertility study 1 (mJS2) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-1.8599863
Number of Samples:7 / 8
Experimental male infertility study 1 (mJS2)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained almost exclusively Sertoli cells. Tissue samples were classified based on modified Johnsen score (mJS) as mJS2.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

influenza virus study 9 (A/pH1N1) / influenza virus study 4 (A/H1N1)

Relative Expression (log2-ratio):1.8568211
Number of Samples:3 / 3
Experimental influenza virus study 9 (A/pH1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype [A/Singapore/478/2009 (pH1N1)]. Samples were taken 10 hours post-infection.
Control influenza virus study 4 (A/H1N1)
Human carcinoma cell line A549 infected with influenza A virus subtype A/WSN/33 (H1N1). Samples were taken 10 hours post-infection.

male infertility study 1 (mJS3) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-1.8075457
Number of Samples:3 / 8
Experimental male infertility study 1 (mJS3)
Human testicular lobules biopsy samples isolated from adult infertile patients whose seminiferous tubules contained Sertoli cells but rarely spermatogonia. Tissue samples were classified based on modified Johnsen score (mJS) as mJS3.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

male infertility study 1 (juvenile; Ad-) / normal testicular lobules tissue (mJS10)

Relative Expression (log2-ratio):-1.7806463
Number of Samples:2 / 8
Experimental male infertility study 1 (juvenile; Ad-)
Human testicular lobules biopsy samples isolated from prepubescent patients with undescended testes. Testes of these children contained very low level of A-dark (Ad-) spermatogonial cells.
Control normal testicular lobules tissue (mJS10)
Biopsies of human testicular lobules isolated from fertile vasectomized adult men with normal spermatogenesis. Histological analysis classified samples as mJS10 (modified Johnsen score 10).

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (subconfluent)

Relative Expression (log2-ratio):1.7663918
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (subconfluent)
Subconfluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 1-2 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells still grew dispersed in the culture insert and covered 70% of the insert.

oligodendrocyte progenitor cell study 2 (O4-;CD140a+) / oligodendrocyte progenitor cell study 2 (O4-;CD140a-)

Relative Expression (log2-ratio):1.743125
Number of Samples:5 / 5
Experimental oligodendrocyte progenitor cell study 2 (O4-;CD140a+)
Fetal oligodendrocyte cells between 20-22wk gestational age derived from fetal brains were FACS sorted on the basis of CD140a (PDGFRA) antigens. Samples were O4-/CD140a+.
Control oligodendrocyte progenitor cell study 2 (O4-;CD140a-)
Fetal oligodendrocyte cells between 20-22wk gestational age derived from fetal brains were FACS sorted on the basis of CD140a (PDGFRA) antigens. Samples were O4-/CD140a-.

CREB1 depletion study 2 (NCI-H3118) / control transduced NCI-H3118 cell sample

Relative Expression (log2-ratio):-1.7095289
Number of Samples:3 / 3
Experimental CREB1 depletion study 2 (NCI-H3118)
NCI-H3118 cells with shRNA mediated knock down of CREB1. Cells were infected on two consecutive dates with the lentiviruses in fresh complete medium (DMEM, 10% FBS) plus 8ug/ml polybrene for 6 to 8 hours and replaced with fresh medium. Cells were harvested at 72 hours after the 1st infection.
Control control transduced NCI-H3118 cell sample
H3118 cells expressing a control shRNA targeting luciferase gene (shLuc). Cells were infected on two consecutive dates with the lentiviruses in fresh complete medium (DMEM, 10% FBS) plus 8ug/ml polybrene for 6 to 8 hours and replaced with fresh medium. Cells were harvested at 72 hours after the 1st infection.

bronchial epithelial cell differentiation study 1 (day28) / bronchial epithelial cell differentiation study 1 (confluent)

Relative Expression (log2-ratio):1.6516466
Number of Samples:3 / 3
Experimental bronchial epithelial cell differentiation study 1 (day28)
Differentiated normal human bronchial epithelial cell (NHBE) cultured for 28 days in an air-liquid interface (ALI) system. At this time point cells were fully differentiated and formed a polarized, pseudostratified mucociliary epithelium. In order to generate the ALI culture, undifferentiated cells were grown under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. When cells reached confluency (after 3-5 days), an air-liquid interface culture (ALI system) was created by removing the apical medium and replacing the medium of the basal compartment with B-ALI differentiation medium. During the 28 days culture medium was replaced every 48 hour.
Control bronchial epithelial cell differentiation study 1 (confluent)
Confluent undifferentiated normal human bronchial epithelial cell (NHBE) cultured for 3-5 days under submerged conditions in bronchial air-liquid interface (B-ALI) growth basal medium supplemented with expansion media. At this time point cells formed a monolayer covering the whole surface of the insert.

succinate dehydrogenase B depletion study 1 (siRNA) / control siRNA transfected Hep3B cell sample

Relative Expression (log2-ratio):1.6443033
Number of Samples:3 / 3
Experimental succinate dehydrogenase B depletion study 1 (siRNA)
Hep3B cells stably transfected with short hairpin vectors containing an siRNA directed to the subunit B of the succinate dehydrogenase.
Control control siRNA transfected Hep3B cell sample
Hep3B cells stably transfected with empty siRNA expression vector (pU6).