TOP TEN perturbations for NM_000306 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000306
Selected probe(set): 207846_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000306 (207846_at) across 5392 perturbations tested by GENEVESTIGATOR:

null cell adenoma study 1 / normal pituitary gland tissue

Relative Expression (log2-ratio):-6.1033926
Number of Samples:2 / 8
Experimental null cell adenoma study 1
Null cell adenoma biopsy samples recovered during transsphenoidal surgery. The tumors were defined by gonadotropic staining for FSH, LH, or alpha subunit in less than 5-10% of cells. Samples were scanned between 04/6/2004 - 15/4/2005.
Control normal pituitary gland tissue
Biopsy samples of healthy pituitary gland recovered post-mortem during autopsy within 2-18 hours of death. Cause of death were mostly various pulmonary or cardiovascular conditions.

gonadotroph adenoma study 1 / normal pituitary gland tissue

Relative Expression (log2-ratio):-5.896358
Number of Samples:7 / 8
Experimental gonadotroph adenoma study 1
Gonadotroph adenoma biopsy samples recovered during transsphenoidal surgery. The tumors were defined as demonstrating positive immunostaining for FSH, LH, or alpha subunit in greater than 5-10% of cells. Samples were scanned between 04/6/2004 - 15/4/2005.
Control normal pituitary gland tissue
Biopsy samples of healthy pituitary gland recovered post-mortem during autopsy within 2-18 hours of death. Cause of death were mostly various pulmonary or cardiovascular conditions.

immune cell study 12 (CD8+ Tc; CPT) / immune cell study 12 (CD8+ Tc; Ficoll)

Relative Expression (log2-ratio):-0.63676214
Number of Samples:3 / 3
Experimental immune cell study 12 (CD8+ Tc; CPT)
CD8+ T-cell samples derived from peripheral blood mononuclear cells that were isolated using the BD vacutainer cell preparation tube (CPT) method. Peripheral blood mononuclear cells were derived from healthy adult donors. The cells were collected using BD vacutainer tubes containing 0.1 M sodium citrate, isolated following the manufacture?s instruction, and were cryopreserved in liquid nitrogen before isolation of CD8+ T-cells via positive selection (antibody-labelled microbeads).
Control immune cell study 12 (CD8+ Tc; Ficoll)
CD8+ T-cell samples derived from peripheral blood mononuclear cells that were isolated using Ficoll-Paque gradient centrifugation. Peripheral blood mononuclear cells were derived from healthy adult donors. The cells were collected using BD vacutainer tubes containing acid-citrate-dextrose anticoagulant (solution A), isolated using the Ficoll method, and were cryopreserved in liquid nitrogen before isolation of CD8+ T-cells via positive selection (antibody-labelled microbeads).

asthma study 22 (neutrophilic AIP) / control sputum sample (neutrophilic AIP)

Relative Expression (log2-ratio):-0.6014538
Number of Samples:17 / 2
Experimental asthma study 22 (neutrophilic AIP)
Sputum samples from asthmatic patients with neutrophilic airway inflammatory phenotype (AIP).
Control control sputum sample (neutrophilic AIP)
Control sputum samples from subjects with neutrophilic airway inflammatory phenotype (AIP).

acrolein study 2 (2800ug/ml) / vehicle (EtOH) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):0.59421206
Number of Samples:3 / 3
Experimental acrolein study 2 (2800ug/ml)
Bronchial epithelial cells (NHBE) treated with 2800 ug/ml acrolein (within range of concentrations reported to induce toxicity in lung epithelial cells and other cell types) for 8 hours. NHBE cells were derived from a 60 year old male non-smoker. ATC code:---
Control vehicle (EtOH) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (ethanol) at a final concentration of 2% v/v (concentration that ensures >80% cell viability after 24 hours of exposure) for 8 hours. NHBE cells were derived from a 60 year old male non-smoker.

systemic onset JIA study 6 (baseline; placebo) / normal blood sample

Relative Expression (log2-ratio):0.5159745
Number of Samples:22 / 22
Experimental systemic onset JIA study 6 (baseline; placebo)
Whole blood samples collected from systemic juvenile idiopathic arthritis patients at baseline (day 1), prior to placebo treatment. Patients were 2 to 19 years of age with a confirmed diagnosis of febrile SJIA with high disease activity. aACR response was scored at day 15. Concomitant therapy with a prednisone equivalent (up to 1.0 mg/kg/day) and stable doses of nonsteroidal anti inflammatory drugs and methotrexate (?20 mg/m2 per week) were permitted.
Control normal blood sample
Whole blood samples collected from healthy volunteers.

TBTO study 1 (24h) / vehicle (EtOH) treated HepG2 cell sample

Relative Expression (log2-ratio):0.45590115
Number of Samples:2 / 6
Experimental TBTO study 1 (24h)
HepG2 cells exposed to 0.02nM tributylinoxide (TBTO) in EtOH solvent for 24 hours. ATC code:---
Control vehicle (EtOH) treated HepG2 cell sample
HepG2 cells exposed to EtOH solvent for 24 hours.

Treg activation study 1 (40min) / unstimulated regulatory T-cell sample

Relative Expression (log2-ratio):0.4074583
Number of Samples:2 / 2
Experimental Treg activation study 1 (40min)
Regulatory T-cells were stimulated for 40min with anti-CD3/anti-CD28/IL-2 (100U/ml). Treg were sorted as CD4+ CD25high cells from peripheral blood of healthy donors.
Control unstimulated regulatory T-cell sample
Unstimulated regulatory T-cell sample derived from sorted CD4+ CD25high cells from peripheral blood of healthy donors.

Langerhans cell histiocytosis study 2 (multifocal) / normal epidermal Langerhans cell sample

Relative Expression (log2-ratio):0.40227127
Number of Samples:2 / 10
Experimental Langerhans cell histiocytosis study 2 (multifocal)
Langerhans cell histiocytes (LCH, CD207+) samples isolated from LCH lesions of patients with multifocal (bone, skin lesions) disease.
Control normal epidermal Langerhans cell sample
Normal epidermal Langerhans cells (CD207+) were isolated from skin of children donors (age < 18 years). The Langerhans cells were isolated from presumably healthy tissue.

glioma study 17 ( small cell glioblastoma; unsorted) / non-tumor cortical tissue

Relative Expression (log2-ratio):-0.39094877
Number of Samples:2 / 4
Experimental glioma study 17 ( small cell glioblastoma; unsorted)
Brain cells isolated from high grade small cell glioblastoma (grade IV). Tumor tissue was dissociated using enzymatic treatment and all cells were used for RNA isolation. Patients were 56 ? 3 years old males.
Control non-tumor cortical tissue
Cortical tissue obtained from patients with epilepsy, but without any manifested brain cancer. The tissue was dissociated using enzymatic treatment, but all brain cell types were used for analyses.