TOP TEN perturbations for NM_000312 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000312
Selected probe(set): 206259_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000312 (206259_at) across 5339 perturbations tested by GENEVESTIGATOR:

Langerhans cell histiocytosis study 1 / normal plasmocytoid dendritic cell sample

Relative Expression (log2-ratio):-3.5530138
Number of Samples:7 / 3
Experimental Langerhans cell histiocytosis study 1
Langerhans cell histiocytes (LCH) were isolated from LCH lesions of patients undergoing surgery. All patients had single system disease, five patients had bone lesion, one had skin lesion and one had mucosal manifestation. Langerhans cells histiocytes were purified by FACS as CD1a+ / CD270 + population with > 95% purity.
Control normal plasmocytoid dendritic cell sample
Normal plasmocytoid dendritic cells were isolated from peripheral blood of healthy adult donors. Plasmocytoid dendritic cells were defined as HLA-DR+ / CD45RAhi / CD11c- / BDCA2+ population.

Hep-G2 / HepaRG

Relative Expression (log2-ratio):3.1178093
Number of Samples:9 / 12
Experimental Hep-G2
Human primary cancer cell line derived from the liver of a patient with hepatocellular carcinoma. Synonyms:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 Cellosaurus code:
Control HepaRG
Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code:

dendritic cell study 6 (gardiquimod; RN486) / dendritic cell study 6 (untreated)

Relative Expression (log2-ratio):-2.934204
Number of Samples:4 / 8
Experimental dendritic cell study 6 (gardiquimod; RN486)
Plasmacytoid dendritic cells (pDCs) collected from blood of healthy donors and treated with gardiquimod (TLR7 agonist) and RN486 (Bruton?s tyrosine kinase; BTK inhibitor) for 3 hours. pDCs were sorted as CD303+, CD123+, CD45+, CD69+, CD40+, CD86+.
Control dendritic cell study 6 (untreated)
Plasmacytoid dendritic cells (pDCs) collected from blood of healthy donors. pDCs were sorted as CD303+, CD123+, CD45+, CD69+, CD40+, CD86+.

dendritic cell study 6 (gardiquimod) / dendritic cell study 6 (untreated)

Relative Expression (log2-ratio):-2.499896
Number of Samples:7 / 8
Experimental dendritic cell study 6 (gardiquimod)
Plasmacytoid dendritic cells (pDCs) collected from blood of healthy donors and treated with gardiquimod (TLR7 agonist) for 3 hours. pDCs were sorted as CD303+, CD123+, CD45+, CD69+, CD40+, CD86+.
Control dendritic cell study 6 (untreated)
Plasmacytoid dendritic cells (pDCs) collected from blood of healthy donors. pDCs were sorted as CD303+, CD123+, CD45+, CD69+, CD40+, CD86+.

clear cell renal cell carcinoma study 4 (stage 3) / normal kidney tissue

Relative Expression (log2-ratio):-2.2891064
Number of Samples:14 / 69
Experimental clear cell renal cell carcinoma study 4 (stage 3)
Primary tumor tissue from patients with stage 3 clear cell renal cell carcinoma (ccRCC).
Control normal kidney tissue
Normal kidney tissue samples obtained from healthy individuals.

AsPC-1 / BxPC-3

Relative Expression (log2-ratio):2.2791376
Number of Samples:3 / 3
Experimental AsPC-1
Human xenograft derived metastatic cancer cell line derived from mouse xenografts initiated with metastatic cells from the ascites derived from a 62 years old female Caucasian patient with pancreatic adenocarcinoma. Synonyms:AsPc-1; Aspc-1; ASPC-1; As-PC1; ASPC1; AsPC1; Aspc1; AsPc1 Cellosaurus code:
Control BxPC-3
Human pancreatic adenocarcinoma cell line derived from a 61 years old female patient. Synonyms:BxPc-3; BXPC-3; Bx-PC3; BXPC3; BxPC3; BxPc3 Cellosaurus code:

clear cell renal cell carcinoma study 4 (stage 1) / normal kidney tissue

Relative Expression (log2-ratio):-2.2721329
Number of Samples:23 / 69
Experimental clear cell renal cell carcinoma study 4 (stage 1)
Primary tumor tissue from patients with stage 1 clear cell renal cell carcinoma (ccRCC).
Control normal kidney tissue
Normal kidney tissue samples obtained from healthy individuals.

TGF-? study 9 / untreated PANC-1 cell sample

Relative Expression (log2-ratio):2.2682457
Number of Samples:3 / 3
Experimental TGF-? study 9
PANC-1 cells were grown to 70% confluency. Thereafter the cells were cultured for one day in serum-free conditions and then incubated for 48 hours with fresh media containing 5nM TGF-?. Before harvesting, cells were washed 3x with PBS and lysed by direct addition of TRIzol reagent for RNA extraction.
Control untreated PANC-1 cell sample
PANC-1 cells were grown to 70% confluency. Thereafter the cells were cultured for one day in serum-free conditions and then incubated for 48 hours with plain fresh media. Before harvesting, cells were washed 3x with PBS and lysed by direct addition of TRIzol reagent for RNA extraction.

4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone study 1 (10000 uM; Hep-G2) / vehicle (PBS) treated Hep-G2 cell sample

Relative Expression (log2-ratio):-2.2036476
Number of Samples:3 / 6
Experimental 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone study 1 (10000 uM; Hep-G2)
Hep-G2 cells exposed to 10000 uM 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (dissolved in PBS) for 72 hours. Cells were exposed to the chemical when 80% confluence was reached. Cells were treated with the IC10 determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (number of replicates, n = 3). ATC code:---
Control vehicle (PBS) treated Hep-G2 cell sample
Hep-G2 cells treated with vehicle (PBS) for 72 hours. Cells were exposed to the vehicle when 80% confluence was reached.

glioma study 16 (LN-18) / normal astrocyte sample

Relative Expression (log2-ratio):2.1267385
Number of Samples:2 / 3
Experimental glioma study 16 (LN-18)
Human glioma cell line LN018 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.