TOP TEN perturbations for NM_000343 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000343
Selected probe(set): 206628_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000343 (206628_at) across 5392 perturbations tested by GENEVESTIGATOR:

PMA study 6 (500ng/ml) / vehicle (DME) treated bronchial epithelial cell sample

Relative Expression (log2-ratio):4.43587
Number of Samples:3 / 17
Experimental PMA study 6 (500ng/ml)
Bronchial epithelial cells (NHBE) treated with tetradecanoylphorbol acetate (500ng/ml; vendor: Sigma / catalog number: P1585 / catalog name: Phorbol 12-myristate 13-acetate [16561-29-8]) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial). ATC code:---
Control vehicle (DME) treated bronchial epithelial cell sample
Bronchial epithelial cells (NHBE) treated with vehicle (DME) for 6 hours. For treatment primary cells were seeded at a density of 50'000 cells/well in pre-coated rat tail collagen type I 96-well plates for 24 hours followed by 4 hours starvation. NHBE cells were isolated from different Caucasian, healthy and non-smoker donors from the airway located above the bifurcation of the lungs (tracheal/bronchial).

keratinocyte differentiation study 2 (KLF4 siRNA) / keratinocyte differentiation study 2

Relative Expression (log2-ratio):-3.361144
Number of Samples:2 / 2
Experimental keratinocyte differentiation study 2 (KLF4 siRNA)
KLF4 (Kruppel-like factor 4) depleted primary neonatal keratinocytes differentiated with 1.2 mM calcium for 3 days. Keratinocytes obtained from freshly isolated foreskin were grown in keratinocyte-SFM medium supplemented with epidermal growth factor and bovine pituitary extract. KLF4 depletion was done using siRNAs: KLF4i(A): CCGAGGAGTTCAACGATCT; KLF4i(B): TGACCAGGCACTACCGTAA. Differentiation was induced at 100% confluency.
Control keratinocyte differentiation study 2
Primary neonatal keratinocytes differentiated with 1.2 mM calcium for 3 days. Keratinocytes obtained from freshly isolated foreskin were grown in keratinocyte-SFM medium supplemented with epidermal growth factor and bovine pituitary extract. Differentiation was induced at 100% confluency.

house dust mite study 2 (allergic) / house dust mite study 2 (non-allergic)

Relative Expression (log2-ratio):2.9193754
Number of Samples:5 / 4
Experimental house dust mite study 2 (allergic)
Primary nasal epithelial cells from subjects with monotypical allergy to house dust mite (HDM) were exposed to house dust mite extract (2?g/ml) for 24 hours. Subjects (19-55 years old) were selected based on skin prick test for HDM and other common allergens. The subjects had refrained from using any medication for their allergy in the 4 weeks before the samples collection. Nasal biopsies for isolation of primary cells were taken from the lower edge of the inferior turbinate, and 2 cm from the anterior end.
Control house dust mite study 2 (non-allergic)
Primary nasal epithelial cells from healthy subjects with no allergies were exposed to house dust mite (HDM) extract (2?g/ml) for 24 hours. Subjects (21-32 years old) were selected based on skin prick test for HDM and other common allergens. Nasal biopsies for isolation of primary cells were taken from the lower edge of the inferior turbinate, and 2 cm from the anterior end.

brodalumab study 3 (intermediate) / untreated skin tissue (psoriasis)

Relative Expression (log2-ratio):-2.6535978
Number of Samples:8 / 2
Experimental brodalumab study 3 (intermediate)
Lesional skin punch biopsies derived from patients with psoriasis. The punch biopsy was obtained from active, lesional skin after treatment with 700mg brodalumab for 15 days. ATC code:---
Control untreated skin tissue (psoriasis)
Untreated lesional skin biopsy samples from patients with psoriasis.

Barrett's esophagus study 3 / esophageal squamous cell carcinoma study 1

Relative Expression (log2-ratio):2.5274248
Number of Samples:17 / 8
Experimental Barrett's esophagus study 3
Esophageal epithelium samples from areas with Barrett's esophagus metaplasia which were recovered by laser capture microdissection.
Control esophageal squamous cell carcinoma study 1
Esophageal squamous cell carcinoma (ESCC) biopsy samples from chemotherapy-naive patients with histological grading G1 (well differentiated) and UICC stage II and III, which undergone esophagectomy.

brodalumab study 3 (late) / untreated skin tissue (psoriasis)

Relative Expression (log2-ratio):-2.4844189
Number of Samples:8 / 2
Experimental brodalumab study 3 (late)
Lesional skin punch biopsies derived from patients with psoriasis. The punch biopsy was obtained from active, lesional skin after treatment with 700mg brodalumab for 43 days. ATC code:---
Control untreated skin tissue (psoriasis)
Untreated lesional skin biopsy samples from patients with psoriasis.

ingenol mebutate study 1 (uninvolved skin; 2d; 0.05%) / uninvolved skin sample (baseline)

Relative Expression (log2-ratio):2.3058424
Number of Samples:6 / 5
Experimental ingenol mebutate study 1 (uninvolved skin; 2d; 0.05%)
Punch biopsies of uninvolved skin obtained from actinic keratosis patients after 2 days treatment with ingenol mebutate 0.05% gel. Samples were taken one day after the second topical application with ingenol mebutate from cca 25-cm2 area of uninvolved, normal-appearing, aged but non-sun-exposed skin on the inner aspect of the upper arm. The biopsies were formalin fixed and paraffin embedded. Patients were ? 18 years of age and had two to five clinically typical, visible, and discrete AK lesions within a contiguous 25 cm2 area on the upper extremity (dorsum of hands or forearm) and with one additional AK lesion located 1 to 5 cm from the AK treatment area. Further the donors had uninvolved skin area on the inner aspect of the upper arm. ATC code:
Control uninvolved skin sample (baseline)
Punch biopsies of uninvolved skin obtained from actinic keratosis (AK) patients before treatment (baseline). Samples were taken from cca 25 cm2 area of uninvolved, normal-appearing, aged but non-sun-exposed skin on the inner aspect of the upper arm. The biopsies were formalin fixed and paraffin embedded. Patients were ? 18 years of age and had two to five clinically typical, visible, and discrete AK lesions within a contiguous 25 cm2 area on the upper extremity (dorsum of hands or forearm) and with one additional AK lesion located 1 to 5 cm from the AK treatment area. Further the donors had uninvolved skin area on the inner aspect of the upper arm.

brodalumab study 3 (late) / brodalumab study 1 (late)

Relative Expression (log2-ratio):-2.2465954
Number of Samples:8 / 4
Experimental brodalumab study 3 (late)
Lesional skin punch biopsies derived from patients with psoriasis. The punch biopsy was obtained from active, lesional skin after treatment with 700mg brodalumab for 43 days. ATC code:---
Control brodalumab study 1 (late)
Lesional skin punch biopsies derived from patients with psoriasis. The punch biopsy was obtained from active, lesional skin after treatment with 140mg brodalumab for 43 days. ATC code:---

psoriasis study 2 (lesional) / psoriasis study 2 (non-lesional)

Relative Expression (log2-ratio):2.2250643
Number of Samples:32 / 28
Experimental psoriasis study 2 (lesional)
Lesional skin biopsies from psoriatic patients.
Control psoriasis study 2 (non-lesional)
Non-lesional skin biopsies from psoriatic patients.

esophageal squamous cell carcinoma study 1 / normal esophageal epithelium sample

Relative Expression (log2-ratio):-2.2205992
Number of Samples:8 / 18
Experimental esophageal squamous cell carcinoma study 1
Esophageal squamous cell carcinoma (ESCC) biopsy samples from chemotherapy-naive patients with histological grading G1 (well differentiated) and UICC stage II and III, which undergone esophagectomy.
Control normal esophageal epithelium sample
Histologically normal esophageal squamous cell epithelium biopsy samples from patients that were investigated for esophageal pain, but diagnosed as healthy.