TOP TEN perturbations for NM_000361 (Homo sapiens)

Organism: Homo sapiens
Gene: NM_000361
Selected probe(set): 203887_s_at
Platform: Affymetrix Human Genome U133 Plus 2.0 Array

Expression of NM_000361 (203887_s_at) across 5392 perturbations tested by GENEVESTIGATOR:

calcitriol study 7 (10nM; 18h) / vehicle treated peripheral blood monocyte sample (18h)

Relative Expression (log2-ratio):5.6521416
Number of Samples:4 / 4
Experimental calcitriol study 7 (10nM; 18h)
Peripheral blood monocyte samples derived from blood of healthy donors and treated with 10 nM calcitriol (1alpha, 25-Dihydroxyvitamin D3; 1,25(OH)2D3) for 18 h. ATC code:,
Control vehicle treated peripheral blood monocyte sample (18h)
Peripheral blood monocyte samples derived from blood of healthy donors and treated with vehicle for 18 h.

calcitriol study 7 (10nM; 18h) / vitamin A study 3 (10nM; 18h)

Relative Expression (log2-ratio):5.4439144
Number of Samples:4 / 4
Experimental calcitriol study 7 (10nM; 18h)
Peripheral blood monocyte samples derived from blood of healthy donors and treated with 10 nM calcitriol (1alpha, 25-Dihydroxyvitamin D3; 1,25(OH)2D3) for 18 h. ATC code:,
Control vitamin A study 3 (10nM; 18h)
Peripheral blood monocyte samples derived from blood of healthy donors and treated with 10 nM ATRA (all-trans retinoic acid, biologically active form of vitamin A) for 18 h. ATC code:---

LPS study 4 / mock treated MONO-MAC-6 cell sample

Relative Expression (log2-ratio):5.0022507
Number of Samples:2 / 2
Experimental LPS study 4
MONO-MAC-6 (MM6) cells were treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells mock treated.

hepatocyte (ESC) / Hep-G2

Relative Expression (log2-ratio):4.8795214
Number of Samples:8 / 9
Experimental hepatocyte (ESC)
Hepatocyte-like cells differentiated from embryonic stem cells (ESC)
Control Hep-G2
Human primary cancer cell line derived from the liver of a patient with hepatocellular carcinoma. Synonyms:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 Cellosaurus code:

hepatocyte-like cell differentiation study 1 (20d; HNF4 depl) / hepatocyte-like cell differentiation study 1 (5d)

Relative Expression (log2-ratio):4.7075987
Number of Samples:3 / 3
Experimental hepatocyte-like cell differentiation study 1 (20d; HNF4 depl)
Hepatocyte-like cell differentiated from human pluripotent, embryonic stem cell line (ES, WA09) and transfected with shRNA targeting HNF4A. ES cells were grown in human ES cell media DMEM/F12 and differentiated to mature hepatocytes for 20 days. Moreover hESc was stable transfected with lentivirus expressing an shRNA that efficiently depletes HNF4A.
Control hepatocyte-like cell differentiation study 1 (5d)
Hepatocyte-like cell differentiated from human pluripotent, embryonic stem cell line (ES, WA09). ES cells were grown in human ES cell media DMEM/F12 and differentiated to definitive endoderm for 5 days.

glioma study 16 (LN-229) / normal astrocyte sample

Relative Expression (log2-ratio):-4.471463
Number of Samples:2 / 3
Experimental glioma study 16 (LN-229)
Human glioma cell line LN229 was cultured in DMEM supplemented with 10% FCS (fetal calf serum) and antibiotics at 37?C and 5% CO2.
Control normal astrocyte sample
Normal brain astrocytes. Clonetics normal human astrocytes (NHA) from Cambrex (primary-derived cultures) and cultured according to the manufacturer's recommendations.

pancreatic islet study 3 (expanded; PPRF) / normal pancreatic islet sample

Relative Expression (log2-ratio):-4.4500666
Number of Samples:2 / 7
Experimental pancreatic islet study 3 (expanded; PPRF)
Human pancreatic islets were isolated from a cadaveric donor. The population was 70% pure in mature islets, which was determined by dithizone staining. Islets cells were expanded according to Pharmaceutical Production Research Facility Protocol (PPRF) for 6 weeks. Expansion phase: Approximately 2,500 islet equivalents were seeded onto 75-cm2 fibronectin-coated tissue culture-treated flasks in a proprietary serum-free medium (PPRF medium). The serum-free PPRF medium was supplemented with 30% serum-free mesenchymal-conditioned medium. When the cell migration resulted in the formation of large-size colonies, the cells were harvested by trypsinization and replated into new tissue culture flasks. Once the cells reached near confluence (80?90%) in monolayer, they were trypsinized, counted, and subcultured at a density of 5,000 cells per cm2. Thereafter, cells were serially passaged using the same protocol.
Control normal pancreatic islet sample
Pancreatic islets were obtained from seven donors aged between 37 and 70 years and body mass index between 22 and 27. Functional islets were cultured in CMRL 1066 supplemented with 10% FCS, 1% glutamine, 5.6 mM glucose, 1 mM HEPES, 110 U/ml penicillin, and 110 g/ml streptomycin for 7 days or immediately processed after isolation.

LPS study 4 (shRNA cycT1) / cycT1 depletion study 2 (shRNA)

Relative Expression (log2-ratio):4.446205
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA cycT1)
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control cycT1 depletion study 2 (shRNA)
MONO-MAC-6 (MM6) cells were transduced with shRNA against cyclin T1 and then mock treated.

hepatocyte (ESC) / HepaRG

Relative Expression (log2-ratio):4.4233737
Number of Samples:8 / 12
Experimental hepatocyte (ESC)
Hepatocyte-like cells differentiated from embryonic stem cells (ESC)
Control HepaRG
Immortalized cancer cell line derived from female patient with hepatocellular carcinoma. Cells can be induced to differentiate into hepatocyte-like cells by exposure to DMSO. Synonyms:Hepa-RG Cellosaurus code:

LPS study 4 (shRNA contr.) / mock treated / transduced MONO-MAC-6 cell sample

Relative Expression (log2-ratio):4.401602
Number of Samples:2 / 2
Experimental LPS study 4 (shRNA contr.)
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then treated with 10 ng/ml lipopolysaccharide (LPS). ATC code:---
Control mock treated / transduced MONO-MAC-6 cell sample
MONO-MAC-6 (MM6) cells were transduced with a control shRNA and then mock treated.